LOCUS       BBa_K932003             2158 bp    DNA     linear       26-Dec-2024
DEFINITION  OmpA-Silicatein-Alpha under Lac promoter
ACCESSION   BBa_K932003
VERSION     BBa_K932003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1344
                     /label=OmpA-SilA
     CDS             227..1344
                     /label=BBa_K932002
     misc_feature    1357..1360
                     /label=conserved
     RBS             1353..1364
                     /label=BBa_B0034
     CDS             1374..1990
                     /label=tetR
     misc_feature    1991..2024
                     /label=SsrA
     misc_feature    2031..2055
                     /label=Help:Barcodes
     CDS             1371..2055
                     /label=BBa_C0040
     stem_loop       2071..2090
                     /label=T7 TE
     misc_feature    2097..2097
                     /label=stop
     polyA_site      2091..2104
                     /label=polya
     misc_feature    2064..2104
                     /label=BBa_B0012
     terminator      2064..2104
                     /label=BBa_B0012
     stem_loop       2125..2147
                     /label=stem_loop
     misc_feature    2113..2158
                     /label=BBa_B0011
     terminator      2113..2158
                     /label=BBa_B0011
     terminator      2064..2158
                     /label=BBa_B0014
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aagctactaa
      241 actggtactg ggcgcggtaa tcctgggttc tactctgctg gcaggttgct ccagcaacgc
      301 taaaatcgat cagggaatta acccgtatgt tggctttgaa atgggttacg actggttagg
      361 tcgtatgccg tacaaaggca gcgttgaaaa cggtgcatac aaagctcagg gcgttcaact
      421 gaccgctaaa ctgggttacc caatcactga cgacctggac atctacactc gtctgggtgg
      481 catggtatgg cgtgcagaca ctaaatccaa cgtttatggt aaaaaccacg acaccggcgt
      541 ttctccggtc ttcgctggcg gtgttgagta cgcgatcact cctgaaatcg ctacccgtct
      601 ggaataccag tggaccaaca acatcggtga cgcacacacc atcggcactc gtccggacaa
      661 cggcggaggt tctggaggag ggagctcgcc tacccggaga ctgtagattg gagaacaaag
      721 ggcgctgtga ctggtatcaa gagccaggga gattgtggtg ccagctatgc attcagtgcc
      781 atgggtgcac ttgaaggaat caatgcactt gctactggaa agctgaccta tctcagtgaa
      841 cagaacatca ttgattgctc tgtaccttat ggtaaccatg gttgcaaggg tggaaacatg
      901 tatgtggctt tcctctatgt tgttgctaac gaaggagttg atgatggggg ttcctatcca
      961 tttagaggaa agcaatccag ttgtacgtat caagagcagt accgtggtgc aagtatgtct
     1021 ggctcagttc aaatcaacag tggtagtgaa tctgatctgg aagcagctgt agccaatgtt
     1081 ggtccagttg cagtagctat tgatggagag tcaaatgctt tcagattcta ttacagtgga
     1141 gtgtacgact cctccagatg ttctagtagc agtctcaacc acgccatggt gatcactggc
     1201 tatggaattt caaataacca ggaatactgg cttgcaaaga acagctgggg tgagaactgg
     1261 ggagaactgg gctatgtgaa gatggccagg aacaagtaca atcaatgtgg gattgctagt
     1321 gatgcctcct accccactct ctagtactag agaaagagga gaaatactag atgtccagat
     1381 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
     1441 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
     1501 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
     1561 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
     1621 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
     1681 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
     1741 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
     1801 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
     1861 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     1921 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     1981 aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt
     2041 gctagtgtag atcactacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2101 tatatactag agagagaata taaaaagcca gattattaat ccggcttttt tattattt
//