LOCUS       BBa_K933000             1656 bp    DNA     linear       17-May-2024
DEFINITION  two start codons coding for RFP and sfGFP reporters
ACCESSION   BBa_K933000
VERSION     BBa_K933000.1  
FEATURES             Location/Qualifiers
     RBS             1..28
                     /label=weak RBS
     misc_feature    29..30
                     /label=start codon RFP (ORF 0)
     misc_feature    39..40
                     /label=start codon sfGFP (ORF +1)
     CDS             41..709
                     /label=41
     misc_feature    710..710
                     /label=1N break in reference frame
     CDS             711..1427
                     /label=sfGFP reporter
     stem_loop       1461..1589
                     /label=double terminator
ORIGIN
        1 cctaggggat ccgttgacgg ctagctcagt cctaggtaca gtgctagctt ctagaccgaa
       61 gccgccctca ctcgttaagc aagatggcaa cttatgaaga tcttatcaaa gagttcatgc
      121 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag
      181 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact caaagttacc aaaggtggtc
      241 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg
      301 ttaaacaccc ggctgacatc ccggactacc tcaaactgtc cttcccggaa ggtttcaaat
      361 gggaacgtgt tcttaacttc gaagacggtg gtgttgttac cgttacccag gactcttccc
      421 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg
      481 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg
      541 aagacggtgc tctcaaaggt gaaatcaaaa tacgtctcaa acttaaagac ggtggtcact
      601 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt
      661 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac
      721 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataaacgtaa aggcgaagaa
      781 ctgtttaccg gtgtggttcc gattctggtg gaactggatg gtgatgttaa tggtcataaa
      841 ttcagcgttc gtggtgaagg cgaaggtgat gccacgaatg gtaaactgac cctgaaattt
      901 atctgcacca caggtaaact gccggttccg tggccgaccc tggttaccac cctgacctat
      961 ggtgttcagt gtttcgcacg ttatccggat catatgaaac agcacgattt ctttaaaagc
     1021 gccatgccgg aaggttatgt tcaggaacgt accattagct ttaaagatga cggcacctat
     1081 aaaacccgtg ccgaagttaa attcgaaggc gataccctgg tgaatcgtat cgaactgaaa
     1141 ggcatcgatt ttaaagagga tggtaatatc ctgggccata aactggaata taattttaac
     1201 agccataacg tgtatatcac cgcagataaa cagaaaaacg gcattaaagc gaactttaaa
     1261 atccgccata atgtggaaga tggtagcgtt cagctggcag atcattatca gcagaatacg
     1321 ccgatcggtg atggtccggt tctgctgccg gataatcatt atctgagcac ccagagcgtt
     1381 ctgagtaaag atccgaatga aaaacgtgat cacatggtgc tgttagagtt cgttaccgca
     1441 gcaggtatta cacatggtat ggatgaactg tataaatgat aaggccggcc gtgctagtgt
     1501 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1561 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1621 cgggtgggcc tttctgcgtt tatacaattg actagt
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