BBa_K933000
1
BBa_K933000
two start codons coding for RFP and sfGFP reporters
2012-09-30T11:00:00Z
2015-05-08T01:13:47Z
This part contains no direct BioBrick parts. It was assembled from several pieces of plasmid DNA in the Salis Lab at Penn State. The dRBS1 vector and pBAC reporter components were assembled from PCR-amplified fragments.
This part was used in a design that tests multiple start codons. The first start codon codes for RFP. The second start codon codes for sfGFP. They are 7 nucleotides apart on the DNA strand. The ribosome binding site translation initiation rate determines which protein is expressed. The part has two restriction enzyme sites flanking the RBS and promoter regions, so varying RBS strengths may be tested.
false
false
_1198_
0
13034
9
It's complicated
false
This design used gBlocks synthesized by IDT. Several CBAR reactions were necessary to achieve the construct.
false
Hannah Jepsen-Burger
annotation2212000
1
double terminator
range2212000
1
1461
1589
annotation2211994
1
weak RBS
range2211994
1
1
28
annotation2211999
1
sfGFP reporter
range2211999
1
711
1427
annotation2211997
1
41
range2211997
1
41
709
annotation2211998
1
1N break in reference frame
range2211998
1
710
710
annotation2211996
1
start codon sfGFP (ORF +1)
range2211996
1
39
40
annotation2211995
1
start codon RFP (ORF 0)
range2211995
1
29
30
BBa_K933000_sequence
1
cctaggggatccgttgacggctagctcagtcctaggtacagtgctagcttctagaccgaagccgccctcactcgttaagcaagatggcaacttatgaagatcttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactcaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctcaaactgtccttcccggaaggtttcaaatgggaacgtgttcttaacttcgaagacggtggtgttgttaccgttacccaggactcttccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctcaaaggtgaaatcaaaatacgtctcaaacttaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaacgtaaaggcgaagaactgtttaccggtgtggttccgattctggtggaactggatggtgatgttaatggtcataaattcagcgttcgtggtgaaggcgaaggtgatgccacgaatggtaaactgaccctgaaatttatctgcaccacaggtaaactgccggttccgtggccgaccctggttaccaccctgacctatggtgttcagtgtttcgcacgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggttatgttcaggaacgtaccattagctttaaagatgacggcacctataaaacccgtgccgaagttaaattcgaaggcgataccctggtgaatcgtatcgaactgaaaggcatcgattttaaagaggatggtaatatcctgggccataaactggaatataattttaacagccataacgtgtatatcaccgcagataaacagaaaaacggcattaaagcgaactttaaaatccgccataatgtggaagatggtagcgttcagctggcagatcattatcagcagaatacgccgatcggtgatggtccggttctgctgccggataatcattatctgagcacccagagcgttctgagtaaagatccgaatgaaaaacgtgatcacatggtgctgttagagttcgttaccgcagcaggtattacacatggtatggatgaactgtataaatgataaggccggccgtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacaattgactagt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z