BBa_K933000 1 BBa_K933000 two start codons coding for RFP and sfGFP reporters 2012-09-30T11:00:00Z 2015-05-08T01:13:47Z This part contains no direct BioBrick parts. It was assembled from several pieces of plasmid DNA in the Salis Lab at Penn State. The dRBS1 vector and pBAC reporter components were assembled from PCR-amplified fragments. This part was used in a design that tests multiple start codons. The first start codon codes for RFP. The second start codon codes for sfGFP. They are 7 nucleotides apart on the DNA strand. The ribosome binding site translation initiation rate determines which protein is expressed. The part has two restriction enzyme sites flanking the RBS and promoter regions, so varying RBS strengths may be tested. false false _1198_ 0 13034 9 It's complicated false This design used gBlocks synthesized by IDT. Several CBAR reactions were necessary to achieve the construct. false Hannah Jepsen-Burger annotation2212000 1 double terminator range2212000 1 1461 1589 annotation2211994 1 weak RBS range2211994 1 1 28 annotation2211999 1 sfGFP reporter range2211999 1 711 1427 annotation2211997 1 41 range2211997 1 41 709 annotation2211998 1 1N break in reference frame range2211998 1 710 710 annotation2211996 1 start codon sfGFP (ORF +1) range2211996 1 39 40 annotation2211995 1 start codon RFP (ORF 0) range2211995 1 29 30 BBa_K933000_sequence 1 cctaggggatccgttgacggctagctcagtcctaggtacagtgctagcttctagaccgaagccgccctcactcgttaagcaagatggcaacttatgaagatcttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactcaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctcaaactgtccttcccggaaggtttcaaatgggaacgtgttcttaacttcgaagacggtggtgttgttaccgttacccaggactcttccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctcaaaggtgaaatcaaaatacgtctcaaacttaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaacgtaaaggcgaagaactgtttaccggtgtggttccgattctggtggaactggatggtgatgttaatggtcataaattcagcgttcgtggtgaaggcgaaggtgatgccacgaatggtaaactgaccctgaaatttatctgcaccacaggtaaactgccggttccgtggccgaccctggttaccaccctgacctatggtgttcagtgtttcgcacgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggttatgttcaggaacgtaccattagctttaaagatgacggcacctataaaacccgtgccgaagttaaattcgaaggcgataccctggtgaatcgtatcgaactgaaaggcatcgattttaaagaggatggtaatatcctgggccataaactggaatataattttaacagccataacgtgtatatcaccgcagataaacagaaaaacggcattaaagcgaactttaaaatccgccataatgtggaagatggtagcgttcagctggcagatcattatcagcagaatacgccgatcggtgatggtccggttctgctgccggataatcattatctgagcacccagagcgttctgagtaaagatccgaatgaaaaacgtgatcacatggtgctgttagagttcgttaccgcagcaggtattacacatggtatggatgaactgtataaatgataaggccggccgtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacaattgactagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z