LOCUS BBa_K934001 4208 bp DNA linear 23-Nov-2024 DEFINITION phaC1-A-B1 [P(3HB) synthesis] ACCESSION BBa_K934001 VERSION BBa_K934001.1 FEATURES Location/Qualifiers CDS 353..2122 /label=phaC1 CDS 2207..3388 /label=phaA CDS 3463..4203 /label=phaB1 promoter 1..50 /label=Pwt RBS 336..342 /label=RBS RBS 2196..2199 /label=RBS RBS 3453..3456 /label=RBS ORIGIN 1 gattcccgca ttgacagcgc gtgcgttgca aggcaacaat ggactcaaat gtctcggaat 61 cgctgacgat tcccaggttt ctccggcaag catagcgcat ggcgtctcca tgcgagaatg 121 tcgcgcttgc cggataaaag gggagccgct atcggaatgg acgcaagcca cggccgcagc 181 aggtgcggtc gagggcttcc agccagttcc agggcagatg tgccggcaga ccctcccgct 241 ttgggggagg cgcaagccgg gtccattcgg atagcatctc cccatgcaaa gtgccggcca 301 gggcaatgcc cggagccggt tcgaatagtg acggcagaga gacaatcaaa tcatggctac 361 tgggaaagga gccgccgcca gcacccagga aggcaaaagc caaccattta aagtgacccc 421 aggcccgttc gatcctgcca cctggctgga atggtcacgt caatggcaag gtacggaggg 481 caacggccat gcggcagcgt ccggcatccc tgggcttgat gcactggctg gtgttaaaat 541 tgcgcctgcc caattgggag acatccaaca gcgttatatg aaagattttt cggcgctgtg 601 gcaggcgatg gcggaaggta aagcagaggc gacgggaccg ctgcatgacc gtcgctttgc 661 tggcgacgcc tggcgtacca atttgcccta tcgttttgcg gcagccttct acctgctgaa 721 cgctcgtgcc ctgactgagc tggcagatgc tgtcgaagcg gatgccaaaa cccgccaacg 781 catccgtttt gccatctcac agtgggtcga cgcaatgtca ccggccaact tcctggccac 841 caatccggaa gcacagcgcc tcctgattga aagcggcggg gaatcgctgc gcgccggcgt 901 tcgtaacatg atggaagatc tgacccgtgg taagatttcg cagacggatg agtcggcctt 961 cgaggtgggt cgcaatgttg cagtgacaga aggcgcggtg gtgtttgaga acgaatactt 1021 tcaacttctc cagtataaac cactgacgga taaagtgcat gcgcgcccat tgttaatggt 1081 tcccccgtgc attaataaat actatatcct ggatcttcag ccggaaagct ccttagtgcg 1141 ccacgtggtg gaacaggggc atactgtgtt tttagttagc tggcgcaatc cagatgcctc 1201 aatggccggc agtacctggg atgactacat tgaacacgcg gctattcgcg cgatcgaagt 1261 ggcacgcgac atctcaggtc aggacaagat taacgtactc ggcttttgcg tcggtggcac 1321 tatcgtgagc accgctctgg cggtactggc tgcgcgtggt gaacaccccg cagcgtcggt 1381 tacgcttctg accacactgt tggatttcgc cgacactggc atcttggatg tatttgtcga 1441 tgagggccac gttcagctgc gcgaggccac tctgggtggc ggcgccggcg caccgtgcgc 1501 tctgctgcgc ggtctggagc tcgcgaatac tttttctttc ttacgcccga acgatcttgt 1561 ctggaattac gtagttgaca actatctcaa aggtaacacg cccgtgccgt ttgatttatt 1621 attctggaat ggggatgcga ccaacctgcc gggtccttgg tattgttggt atctccgtca 1681 tacatactta cagaatgaat taaaggtccc aggtaaactt actgtgtgtg gtgtcccggt 1741 agatctggcg agtatcgacg tgccgaccta catttatggg agtcgtgaag atcatattgt 1801 gccgtggacc gccgcgtacg cttctaccgc gcttctggca aacaaattac gtttcgttct 1861 gggtgccagc ggccacattg cgggtgttat taatcctccg gccaaaaata aacgctcgca 1921 ttggacaaac gacgccttgc cggagagccc tcagcagtgg ttggcgggtg ccattgagca 1981 ccatggaagt tggtggccgg actggacagc ctggctggcg ggccaagcag gcgcaaaacg 2041 cgctgcgcca gccaactatg gcaacgcgcg ctaccgcgcg attgaacccg cgcctggccg 2101 ctatgtaaaa gcaaaagcgt gacgcttgca tgagtgccgg cgtgcgtcat gcacggcgcc 2161 ggcaggcctg cacgttccct cccgtttcca ttgaaaggac tacacaatga ccgatgttgt 2221 tatcgtcagt gccgcgcgca ccgccgtggg taaatttggc ggcagtctgg cgaaaatccc 2281 ggcgccagaa ttgggcgcgg tggtaatcaa agcggccctg gaacgcgccg gtgtgaaacc 2341 tgagcaggtg agcgaagtaa ttatgggtca ggttctgacc gcgggcagcg ggcaaaatcc 2401 ggcgcgtcag gctgcgatta aggcaggcct tccggcgatg gtgcccgcga tgaccattaa 2461 caaagtatgc ggatcgggac tgaaagcagt gatgctggcc gctaacgcca ttatggcagg 2521 cgacgcggaa attgtggtcg ctggcggaca ggagaatatg tcagccgcgc ctcatgtgct 2581 gccgggtagc cgcgacggct ttcgcatggg tgatgcaaaa ctggtcgata ctatgattgt 2641 tgacggctta tgggatgtgt acaaccagta ccacatgggc atcaccgccg agaacgtggc 2701 caaggaatac ggcatcacac gcgaggcgca ggatgagttc gccgtcggct cgcagaacaa 2761 ggccgaagcc gcgcagaagg ccggcatgtt tgacgaagag atcgtcccgg tgctgatccc 2821 gcagcgcaag ggcgacccgg tggccttcaa gaccgacgag ttcgtgcgcc agggcgccac 2881 gctggacagc atgtccggcc tcaagcccgc cttcgacaag gccggcacgg tgaccgcggc 2941 caacgcctcg ggcctgaacg acggcgccgc cgcggtggtg gtgatgtcgg cggccaaggc 3001 caaggaactg ggcctgaccc cgctggccac gatcaagagc tatgccaacg ccggtgtcga 3061 tcccaaggtg atgggcatgg gcccggtgcc ggcctccaag cgcgccctgt cgcgcgccga 3121 gtggaccccg caagacctgg acctgatgga gatcaacgag gcctttgccg cgcaggcgct 3181 ggcggtgcac cagcagatgg gctgggacac ctccaaggtc aatgtgaacg gcggcgccat 3241 cgccatcggc cacccgatcg gcgcgtcggg ctgccgtatc ctggtgacgc tgctgcacga 3301 gatgaagcgc cgtgacgcga agaagggcct ggcctcgctg tgcatcggcg gcggcatggg 3361 cgtggcgctg gcagtcgagc gcaaataagg aaggggtttt ccggggccgc gcgcggttgg 3421 cgcggacccg gcgacgataa cgaagccaat caaggagtgg acatgactca gcgcattgcg 3481 tatgtgaccg gcggcatggg tggtatcgga accgccattt gccagcggct ggccaaggat 3541 ggctttcgtg tggtggccgg ttgcggcccc aactcgccgc gccgcgaaaa gtggctggag 3601 cagcagaagg ccctgggctt cgatttcatt gcctcggaag gcaatgtggc tgactgggac 3661 tcgaccaaga ccgcattcga caaggtcaag tccgaggtcg gcgaggttga tgtgctgatc 3721 aacaacgccg gtatcacccg cgacgtggtg ttccgcaaga tgacccgcgc cgactgggat 3781 gcggtgatcg acaccaacct gacctcgctg ttcaacgtca ccaagcaggt gatcgacggc 3841 atggccgacc gtggctgggg ccgcatcgtc aacatctcgt cggtgaacgg gcagaagggc 3901 cagttcggcc agaccaacta ctccaccgcc aaggccggcc tgcatggctt caccatggca 3961 ctggcgcagg aagtggcgac caagggcgtg accgtcaaca cggtctctcc gggctatatc 4021 gccaccgaca tggtcaaggc gatccgccag gacgtgctcg acaagatcgt cgcgacgatc 4081 ccggtcaagc gcctgggcct gccggaagag atcgcctcga tctgcgcctg gttgtcgtcg 4141 gaggagtccg gtttctcgac cggcgccgac ttctcgctca acggcggcct gcatatgggc 4201 tgacctgc //