LOCUS       BBa_K934016             1332 bp    DNA     linear       22-Nov-2024
DEFINITION  Plas-LacI
ACCESSION   BBa_K934016
VERSION     BBa_K934016.1  
FEATURES             Location/Qualifiers
     promoter        1..83
                     /label=lasI promoter
     promoter        1..83
                     /label=BBa_K649000
     misc_feature    96..99
                     /label=conserved
     RBS             92..103
                     /label=BBa_B0034
     CDS             110..1195
                     /label=BBa_I732100
     stem_loop       1215..1258
                     /label=stem_loop
     misc_feature    1204..1283
                     /label=BBa_B0010
     terminator      1204..1283
                     /label=BBa_B0010
     stem_loop       1299..1318
                     /label=T7 TE
     misc_feature    1325..1325
                     /label=stop
     polyA_site      1319..1332
                     /label=polya
     misc_feature    1292..1332
                     /label=BBa_B0012
     terminator      1292..1332
                     /label=BBa_B0012
     misc_feature    92..1332
                     /label=BBa_I732820
ORIGIN
        1 ttcgagccta gcaagggtcc gggttcaccg aaatctatct catttgctag ttataaaatt
       61 atgaaatttg cgtaaattct tcatactaga gaaagaggag aaatactaga tgaaaccagt
      121 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt
      181 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga
      241 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat
      301 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa
      361 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt
      421 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat
      481 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc
      541 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga
      601 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct
      661 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata
      721 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc
      781 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt
      841 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt
      901 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc
      961 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     1021 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     1081 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     1141 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt aataatacta
     1201 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1261 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1321 tctgcgttta ta
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