##gff-version 3
##sequence-region BBa_K934023 1 1804
BBa_K934023	.	engineered_region	807	1804	.	+	0	ID=BBa_S03119;Name=BBa_S03119
BBa_K934023	.	terminator	1764	1804	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03119
BBa_K934023	.	stem_loop	1771	1790	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K934023	.	polyA_site	1791	1804	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K934023	.	stop_codon	1797	1797	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K934023	.	engineered_region	1764	1804	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K934023	.	promoter	807	860	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03119
BBa_K934023	.	engineered_region	807	860	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K934023	.	promoter	826	831	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K934023	.	non_covalent_binding_site	832	850	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K934023	.	promoter	849	854	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K934023	.	non_covalent_binding_site	807	825	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K934023	.	terminator	1676	1755	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03119
BBa_K934023	.	stem_loop	1687	1730	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K934023	.	engineered_region	1676	1755	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K934023	.	ribosome_entry_site	869	880	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03119
BBa_K934023	.	conserved_region	873	876	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K934023	.	CDS	887	1642	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S03119
BBa_K934023	.	engineered_region	887	888	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K934023	.	engineered_tag	1643	1667	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K934023	.	CDS	887	1636	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K934023	.	point_mutation	1378	1378	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K934023	.	point_mutation	1060	1060	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K934023	.	engineered_region	887	1642	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K934023	.	engineered_region	1	798	.	+	0	ID=BBa_K934022;Name=BBa_K934022
BBa_K934023	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K934022
BBa_K934023	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K934023	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K934023	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K934023	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K934023	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K934023	.	engineered_region	64	798	.	+	0	ID=BBa_K081016;Name=BBa_K081016;Parent=BBa_K934022
BBa_K934023	.	terminator	760	798	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081016
BBa_K934023	.	polyA_site	760	768	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K934023	.	polyA_site	791	798	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K934023	.	engineered_region	760	798	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K934023	.	stem_loop	769	790	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K934023	.	ribosome_entry_site	64	78	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081016
BBa_K934023	.	engineered_region	64	78	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K934023	.	ribosome_entry_site	71	75	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K934023	.	ribosome_entry_site	64	78	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K934023	.	CDS	85	751	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_K081016
BBa_K934023	.	tag	688	720	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K934023	.	CDS	85	687	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K934023	.	engineered_tag	727	751	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K934023	.	CDS	85	726	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_K934023	.	tag	688	720	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K934023	.	CDS	85	687	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K934023	.	engineered_tag	727	751	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
>BBa_K934023
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaat
actagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctca
ggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccg
tactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgt
acatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccg
tttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgt
tactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtc
tggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaa
aacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgct
ctggttgcttaataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcgggg
tttttttttactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagaga
aagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaag
caataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttat
cctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgcta
atttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaa
tgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctatt
catacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttac
atgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatc
aaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaa
atattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgcc
aaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtg
tagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata