LOCUS BBa_K934026 1304 bp DNA linear 05-Nov-2024 DEFINITION Plux-LacI ACCESSION BBa_K934026 VERSION BBa_K934026.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..1167 /label=BBa_I732100 stem_loop 1187..1230 /label=stem_loop misc_feature 1176..1255 /label=BBa_B0010 terminator 1176..1255 /label=BBa_B0010 stem_loop 1271..1290 /label=T7 TE misc_feature 1297..1297 /label=stop polyA_site 1291..1304 /label=polya misc_feature 1264..1304 /label=BBa_B0012 terminator 1264..1304 /label=BBa_B0012 misc_feature 64..1304 /label=BBa_I732820 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc 121 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 181 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 241 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 301 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 361 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 421 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 481 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 541 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 601 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 661 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 721 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 781 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 841 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 901 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 961 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 1021 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 1141 tcccgactgg aaagcgggca gtaataatac tagagccagg catcaaataa aacgaaaggc 1201 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1261 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //