LOCUS       BBa_K935001              655 bp    DNA     linear       09-May-2024
DEFINITION  E. coli chromosomal ars promoter with arsR repressor gene and 
            double terminator (B0010-B0012)
ACCESSION   BBa_K935001
VERSION     BBa_K935001.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     stem_loop       538..581
                     /label=stem_loop
     misc_feature    527..606
                     /label=BBa_B0010
     terminator      527..606
                     /label=BBa_B0010
     stem_loop       622..641
                     /label=T7 TE
     misc_feature    648..648
                     /label=stop
     polyA_site      642..655
                     /label=polya
     misc_feature    615..655
                     /label=BBa_B0012
     terminator      615..655
                     /label=BBa_B0012
     terminator      527..655
                     /label=BBa_B0015
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagagccag gcatcaaata
      541 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      601 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//