LOCUS       BBa_K935002             1604 bp    DNA     linear       25-Nov-2024
DEFINITION  E. coli ArsR under the control of pArs coupled with pArs + RFP
ACCESSION   BBa_K935002
VERSION     BBa_K935002.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     stem_loop       538..581
                     /label=stem_loop
     misc_feature    527..606
                     /label=BBa_B0010
     terminator      527..606
                     /label=BBa_B0010
     stem_loop       622..641
                     /label=T7 TE
     misc_feature    648..648
                     /label=stop
     polyA_site      642..655
                     /label=polya
     misc_feature    615..655
                     /label=BBa_B0012
     terminator      615..655
                     /label=BBa_B0012
     terminator      527..655
                     /label=BBa_B0015
     misc_feature    1..655
                     /label=BBa_K935001
     misc_feature    672..704
                     /label=ArsR binding site
     promoter        701..735
                     /label=-35 and -10 region
     promoter        664..735
                     /label=BBa_K190015
     misc_feature    748..751
                     /label=conserved
     RBS             744..755
                     /label=BBa_B0034
     CDS             762..1436
                     /label=mrfp1
     misc_feature    1443..1467
                     /label=Help:Barcodes
     CDS             762..1467
                     /label=BBa_E1010
     stem_loop       1487..1530
                     /label=stem_loop
     misc_feature    1476..1555
                     /label=BBa_B0010
     terminator      1476..1555
                     /label=BBa_B0010
     stem_loop       1571..1590
                     /label=T7 TE
     misc_feature    1597..1597
                     /label=stop
     polyA_site      1591..1604
                     /label=polya
     misc_feature    1564..1604
                     /label=BBa_B0012
     terminator      1564..1604
                     /label=BBa_B0012
     terminator      1476..1604
                     /label=BBa_B0015
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagagccag gcatcaaata
      541 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      601 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
      661 gagctgcact tacacattcg ttaagtcata tatgtttttg acttatccgc ttcgaagaga
      721 gacactacct gcaattacta gagaaagagg agaaatacta gatggcttcc tccgaagacg
      781 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
      841 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
      901 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
      961 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
     1021 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
     1081 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
     1141 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
     1201 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1261 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     1321 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     1381 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     1441 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc
     1501 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1561 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//