LOCUS       BBa_K935004             2050 bp    DNA     linear       09-May-2024
DEFINITION  E. coli ArsR generator coupled to pArs-ArsR-RFP
ACCESSION   BBa_K935004
VERSION     BBa_K935004.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     stem_loop       538..581
                     /label=stem_loop
     misc_feature    527..606
                     /label=BBa_B0010
     terminator      527..606
                     /label=BBa_B0010
     stem_loop       622..641
                     /label=T7 TE
     misc_feature    648..648
                     /label=stop
     polyA_site      642..655
                     /label=polya
     misc_feature    615..655
                     /label=BBa_B0012
     terminator      615..655
                     /label=BBa_B0012
     terminator      527..655
                     /label=BBa_B0015
     misc_feature    699..717
                     /label=ArsR binding site
     promoter        720..725
                     /label=-35 sequence
     promoter        743..748
                     /label=-10 sequence
     RBS             771..775
                     /label=rbs
     CDS             782..1135
                     /label=arsR
     misc_feature    664..1181
                     /label=BBa_J33201
     misc_feature    1194..1197
                     /label=conserved
     RBS             1190..1201
                     /label=BBa_B0034
     CDS             1208..1882
                     /label=mrfp1
     misc_feature    1889..1913
                     /label=Help:Barcodes
     CDS             1208..1913
                     /label=BBa_E1010
     stem_loop       1933..1976
                     /label=stem_loop
     misc_feature    1922..2001
                     /label=BBa_B0010
     terminator      1922..2001
                     /label=BBa_B0010
     stem_loop       2017..2036
                     /label=T7 TE
     misc_feature    2043..2043
                     /label=stop
     polyA_site      2037..2050
                     /label=polya
     misc_feature    2010..2050
                     /label=BBa_B0012
     terminator      2010..2050
                     /label=BBa_B0012
     terminator      1922..2050
                     /label=BBa_B0015
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagagccag gcatcaaata
      541 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      601 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
      661 gagccaactc aaaattcaca cctattacct tcctctgcac ttacacattc gttaagtcat
      721 atatgttttt gacttatccg cttcgaagag agacactacc tgcaacaatc aggagcgcaa
      781 tatgtcattt ctgttaccca tccaattgtt caaaattctt gctgatgaaa cccgtctggg
      841 catcgtttta ctgctcagcg aactgggaga gttatgcgtc tgcgatctct gcactgctct
      901 cgaccagtcg cagcccaaga tctcccgcca cctggcattg ctgcgtgaaa gcgggctatt
      961 gctggaccgc aagcaaggta agtgggttca ttaccgctta tcaccgcata ttccagcatg
     1021 ggcggcgaaa attattgatg aggcctggcg atgtgaacag gaaaaggttc aggcgattgt
     1081 ccgcaacctg gctcgacaaa actgttccgg ggacagtaag aacatttgca gttaaaaatt
     1141 tagctaaaca catatgaatt ttcagatgtg ttttatccgg gtactagaga aagaggagaa
     1201 atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat
     1261 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
     1321 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
     1381 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
     1441 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
     1501 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
     1561 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
     1621 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa
     1681 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
     1741 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
     1801 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga
     1861 aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga
     1921 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1981 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2041 tgcgtttata
//