LOCUS BBa_K935005 2713 bp DNA linear 08-May-2024 DEFINITION Two E. coli ArsR generators coupled to pArs-ArsR-RFP ACCESSION BBa_K935005 VERSION BBa_K935005.1 FEATURES Location/Qualifiers misc_feature 36..54 /label=ArsR binding site promoter 57..62 /label=-35 sequence promoter 80..85 /label=-10 sequence RBS 108..112 /label=rbs CDS 119..472 /label=arsR misc_feature 1..518 /label=BBa_J33201 stem_loop 538..581 /label=stem_loop misc_feature 527..606 /label=BBa_B0010 terminator 527..606 /label=BBa_B0010 stem_loop 622..641 /label=T7 TE misc_feature 648..648 /label=stop polyA_site 642..655 /label=polya misc_feature 615..655 /label=BBa_B0012 terminator 615..655 /label=BBa_B0012 terminator 527..655 /label=BBa_B0015 misc_feature 699..717 /label=ArsR binding site promoter 720..725 /label=-35 sequence promoter 743..748 /label=-10 sequence RBS 771..775 /label=rbs CDS 782..1135 /label=arsR misc_feature 664..1181 /label=BBa_J33201 stem_loop 1201..1244 /label=stem_loop misc_feature 1190..1269 /label=BBa_B0010 terminator 1190..1269 /label=BBa_B0010 stem_loop 1285..1304 /label=T7 TE misc_feature 1311..1311 /label=stop polyA_site 1305..1318 /label=polya misc_feature 1278..1318 /label=BBa_B0012 terminator 1278..1318 /label=BBa_B0012 terminator 1190..1318 /label=BBa_B0015 misc_feature 1362..1380 /label=ArsR binding site promoter 1383..1388 /label=-35 sequence promoter 1406..1411 /label=-10 sequence RBS 1434..1438 /label=rbs CDS 1445..1798 /label=arsR misc_feature 1327..1844 /label=BBa_J33201 misc_feature 1857..1860 /label=conserved RBS 1853..1864 /label=BBa_B0034 CDS 1871..2545 /label=mrfp1 misc_feature 2552..2576 /label=Help:Barcodes CDS 1871..2576 /label=BBa_E1010 stem_loop 2596..2639 /label=stem_loop misc_feature 2585..2664 /label=BBa_B0010 terminator 2585..2664 /label=BBa_B0010 stem_loop 2680..2699 /label=T7 TE misc_feature 2706..2706 /label=stop polyA_site 2700..2713 /label=polya misc_feature 2673..2713 /label=BBa_B0012 terminator 2673..2713 /label=BBa_B0012 terminator 2585..2713 /label=BBa_B0015 ORIGIN 1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata 61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat 121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat 181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga 241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct 301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc 361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg 421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag 481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagagccag gcatcaaata 541 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 601 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 661 gagccaactc aaaattcaca cctattacct tcctctgcac ttacacattc gttaagtcat 721 atatgttttt gacttatccg cttcgaagag agacactacc tgcaacaatc aggagcgcaa 781 tatgtcattt ctgttaccca tccaattgtt caaaattctt gctgatgaaa cccgtctggg 841 catcgtttta ctgctcagcg aactgggaga gttatgcgtc tgcgatctct gcactgctct 901 cgaccagtcg cagcccaaga tctcccgcca cctggcattg ctgcgtgaaa gcgggctatt 961 gctggaccgc aagcaaggta agtgggttca ttaccgctta tcaccgcata ttccagcatg 1021 ggcggcgaaa attattgatg aggcctggcg atgtgaacag gaaaaggttc aggcgattgt 1081 ccgcaacctg gctcgacaaa actgttccgg ggacagtaag aacatttgca gttaaaaatt 1141 tagctaaaca catatgaatt ttcagatgtg ttttatccgg gtactagagc caggcatcaa 1201 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 1261 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 1321 ctagagccaa ctcaaaattc acacctatta ccttcctctg cacttacaca ttcgttaagt 1381 catatatgtt tttgacttat ccgcttcgaa gagagacact acctgcaaca atcaggagcg 1441 caatatgtca tttctgttac ccatccaatt gttcaaaatt cttgctgatg aaacccgtct 1501 gggcatcgtt ttactgctca gcgaactggg agagttatgc gtctgcgatc tctgcactgc 1561 tctcgaccag tcgcagccca agatctcccg ccacctggca ttgctgcgtg aaagcgggct 1621 attgctggac cgcaagcaag gtaagtgggt tcattaccgc ttatcaccgc atattccagc 1681 atgggcggcg aaaattattg atgaggcctg gcgatgtgaa caggaaaagg ttcaggcgat 1741 tgtccgcaac ctggctcgac aaaactgttc cggggacagt aagaacattt gcagttaaaa 1801 atttagctaa acacatatga attttcagat gtgttttatc cgggtactag agaaagagga 1861 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg 1921 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc 1981 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc 2041 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc 2101 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat 2161 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga 2221 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca 2281 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct 2341 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt 2401 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat 2461 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc 2521 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact 2581 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 2641 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2701 ttctgcgttt ata //