BBa_K935005
type: ComponentDefinition
persistent identity: BBa K935005
display id: BBa_K935005
version: 1
was generated by: iGEM to SBOL conversion
description: Two E. coli ArsR generators coupled to pArs-ArsR-RFP
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:47Z
mutable provenance: The IGEM team
owned by: james
owned by: myers
mutable description: This part created
discontinued: false
dominant: false
experience: experience/None
group u list: _1200_
m user id: 0
owner id: 14627
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: RFP promoter
star: false
creator: Twaddle Lab
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J33201
component: BBa_B0015
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_J33201
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K935005_sequence/1

BBa_J33201
type: Component
persistent identity: component2295927
display id: component2295927
version: 1
top level: BBa_K935005
definition: BBa_J33201
access: public

BBa_B0015
type: Component
persistent identity: component2295921
display id: component2295921
version: 1
top level: BBa_K935005
definition: BBa_B0015
access: public

BBa_J33201
type: Component
persistent identity: component2295940
display id: component2295940
version: 1
top level: BBa_K935005
definition: BBa_J33201
access: public

BBa_B0015
type: Component
persistent identity: component2295934
display id: component2295934
version: 1
top level: BBa_K935005
definition: BBa_B0015
access: public

BBa_E1010
type: Component
persistent identity: component2295945
display id: component2295945
version: 1
top level: BBa_K935005
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2295942
display id: component2295942
version: 1
top level: BBa_K935005
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2295952
display id: component2295952
version: 1
top level: BBa_K935005
definition: BBa_B0015
access: public

BBa_J33201
type: Component
persistent identity: component2295914
display id: component2295914
version: 1
top level: BBa_K935005
definition: BBa_J33201
access: public

BBa_J33201
type: SequenceAnnotation
persistent identity: annotation2295914
display id: annotation2295914
version: 1
top level: BBa_K935005
location: https://synbiohub.org/public/igem/BBa_K935005/annotation2295914/range2295914/1
component: BBa_J33201

https://synbiohub.org/public/igem/BBa_K935005/annotation2295914/range2295914/1
type: Range
persistent identity: range2295914
display id: range2295914
version: 1
top level: BBa_K935005
direction: direction/reverse
start: 1
end: 518
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2295921
display id: annotation2295921
version: 1
top level: BBa_K935005
location: https://synbiohub.org/public/igem/BBa_K935005/annotation2295921/range2295921/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K935005/annotation2295921/range2295921/1
type: Range
persistent identity: range2295921
display id: range2295921
version: 1
top level: BBa_K935005
direction: direction/reverse
start: 527
end: 655
orientation: inline

BBa_J33201
type: SequenceAnnotation
persistent identity: annotation2295927
display id: annotation2295927
version: 1
top level: BBa_K935005
location: https://synbiohub.org/public/igem/BBa_K935005/annotation2295927/range2295927/1
component: BBa_J33201

https://synbiohub.org/public/igem/BBa_K935005/annotation2295927/range2295927/1
type: Range
persistent identity: range2295927
display id: range2295927
version: 1
top level: BBa_K935005
direction: direction/reverse
start: 664
end: 1181
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2295934
display id: annotation2295934
version: 1
top level: BBa_K935005
location: https://synbiohub.org/public/igem/BBa_K935005/annotation2295934/range2295934/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K935005/annotation2295934/range2295934/1
type: Range
persistent identity: range2295934
display id: range2295934
version: 1
top level: BBa_K935005
direction: direction/reverse
start: 1190
end: 1318
orientation: inline

BBa_J33201
type: SequenceAnnotation
persistent identity: annotation2295940
display id: annotation2295940
version: 1
top level: BBa_K935005
location: https://synbiohub.org/public/igem/BBa_K935005/annotation2295940/range2295940/1
component: BBa_J33201

https://synbiohub.org/public/igem/BBa_K935005/annotation2295940/range2295940/1
type: Range
persistent identity: range2295940
display id: range2295940
version: 1
top level: BBa_K935005
direction: direction/reverse
start: 1327
end: 1844
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2295942
display id: annotation2295942
version: 1
top level: BBa_K935005
location: https://synbiohub.org/public/igem/BBa_K935005/annotation2295942/range2295942/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K935005/annotation2295942/range2295942/1
type: Range
persistent identity: range2295942
display id: range2295942
version: 1
top level: BBa_K935005
direction: direction/reverse
start: 1853
end: 1864
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2295945
display id: annotation2295945
version: 1
top level: BBa_K935005
location: https://synbiohub.org/public/igem/BBa_K935005/annotation2295945/range2295945/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K935005/annotation2295945/range2295945/1
type: Range
persistent identity: range2295945
display id: range2295945
version: 1
top level: BBa_K935005
direction: direction/reverse
start: 1871
end: 2576
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2295952
display id: annotation2295952
version: 1
top level: BBa_K935005
location: https://synbiohub.org/public/igem/BBa_K935005/annotation2295952/range2295952/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K935005/annotation2295952/range2295952/1
type: Range
persistent identity: range2295952
display id: range2295952
version: 1
top level: BBa_K935005
direction: direction/reverse
start: 2585
end: 2713
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J33201
type: ComponentDefinition
persistent identity: BBa J33201
display id: BBa_J33201
version: 1
was generated by: iGEM to SBOL conversion
description: E. coli chromosomal ars promoter with arsR repressor gene
created: 2006-10-12T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: The DNA was derived by PCR using genomic DNA from E. coli JM109. Primers were designed based on the sequence reported by Diorio et al (1995), J. Bacteriol 177 (8), 2050-2056, Genbank accession X80057, GI:510824. The sequence given here is derived by sequencing the biobrick construct.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part consists of the promoter of the E. coli JM109 chromosomal arsenic detoxification operon (ars operon), including the ArsR repressor binding site and the arsR gene encoding the arsR repressor protein, together with its ribosome binding site. Addition of any other genes to the 3' end of this part will result in their expression being dependent on the presence of sodium arsenate or sodium arsenite. Arsenite or arsenite anion binds to the repressor protein ArsR, resulting in inability to repress the promoter. Based on our experiments, a concentration of 1 micromolar sodium arsenate in LB is sufficient for essentially full expression, though this will vary according to conditions.
discontinued: false
dominant: true
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Note that this sequence includes the arsR gene encoding the ArsR repressor protein, which is thus negatively autoregulated. No additional parts are required for arsenate-induced expression from this part. In principle, this part should also function in hosts other than E. coli.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ArsR binding site
sequence annotation: -35 sequence
sequence annotation: -10 sequence
sequence annotation: rbs
sequence annotation: arsR
sequence: https://synbiohub.org/public/igem/BBa_J33201_sequence/1

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation1902812
display id: annotation1902812
version: 1
top level: BBa_J33201
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902812/range1902812/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J33201/annotation1902812/range1902812/1
type: Range
persistent identity: range1902812
display id: range1902812
version: 1
top level: BBa_J33201
direction: direction/forward
start: 36
end: 54
orientation: inline

-35 sequence
type: SequenceAnnotation
persistent identity: annotation1902813
display id: annotation1902813
version: 1
top level: BBa_J33201
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902813/range1902813/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J33201/annotation1902813/range1902813/1
type: Range
persistent identity: range1902813
display id: range1902813
version: 1
top level: BBa_J33201
direction: direction/forward
start: 57
end: 62
orientation: inline

-10 sequence
type: SequenceAnnotation
persistent identity: annotation1902814
display id: annotation1902814
version: 1
top level: BBa_J33201
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902814/range1902814/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J33201/annotation1902814/range1902814/1
type: Range
persistent identity: range1902814
display id: range1902814
version: 1
top level: BBa_J33201
direction: direction/forward
start: 80
end: 85
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1902815
display id: annotation1902815
version: 1
top level: BBa_J33201
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902815/range1902815/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J33201/annotation1902815/range1902815/1
type: Range
persistent identity: range1902815
display id: range1902815
version: 1
top level: BBa_J33201
direction: direction/forward
start: 108
end: 112
orientation: inline

arsR
type: SequenceAnnotation
persistent identity: annotation1902816
display id: annotation1902816
version: 1
top level: BBa_J33201
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902816/range1902816/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J33201/annotation1902816/range1902816/1
type: Range
persistent identity: range1902816
display id: range1902816
version: 1
top level: BBa_J33201
direction: direction/forward
start: 119
end: 472
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K935005_sequence/1
type: Sequence
persistent identity: BBa K935005 sequence
display id: BBa_K935005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaaaaatttagctaaacacatatgaattttcagatgtgttttatccgggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaaaaatttagctaaacacatatgaattttcagatgtgttttatccgggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaaaaatttagctaaacacatatgaattttcagatgtgttttatccgggtactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J33201_sequence/1
type: Sequence
persistent identity: BBa J33201 sequence
display id: BBa_J33201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaaaaatttagctaaacacatatgaattttcagatgtgttttatccggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z