LOCUS       BBa_K936019             1806 bp    DNA     linear       15-Dec-2024
DEFINITION  R0010+B0034+Dehydrogenase+B0015
ACCESSION   BBa_K936019
VERSION     BBa_K936019.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1669
                     /label=BBa_K936011
     stem_loop       1689..1732
                     /label=stem_loop
     misc_feature    1678..1757
                     /label=BBa_B0010
     terminator      1678..1757
                     /label=BBa_B0010
     stem_loop       1773..1792
                     /label=T7 TE
     misc_feature    1799..1799
                     /label=stop
     polyA_site      1793..1806
                     /label=polya
     misc_feature    1766..1806
                     /label=BBa_B0012
     terminator      1766..1806
                     /label=BBa_B0012
     terminator      1678..1806
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt cagtacccgt
      241 tcaacatcct atgtatatcg atggacagtt tgttacctgg cgtggagacg catggattga
      301 tgtggtaaac cctgctacag aggctgtcat ttcccgcata cccgatggtc aggccgagga
      361 tgcccgtaag gcaatcgatg cagcagaacg tgcacaacca gaatgggaag cgttgcctgc
      421 tattgaacgc gccagttggt tgcgcaaaat ctccgccggg atccgcgaac gcgccagtga
      481 aatcagtgcg ctgattgttg aagaaggggg caagatccag cagctggctg aagtcgaagt
      541 ggcttttact gccgactata tcgattacat ggcggagtgg gcacggcgtt acgagggcga
      601 gattattcaa agcgatcgtc caggagaaaa tattcttttg tttaaacgtg cgcttggtgt
      661 gactaccggc attctgccgt ggaacttccc gttcttcctc attgcccgca aaatggctcc
      721 cgctcttttg accggtaata tcatcgtcat taaacctagt gaatttacgc caaacaatgc
      781 gattgcattc gccaaaatcg tcgatgaaat aggccttccg cgcggcgtgt ttaaccttgt
      841 actggggcgt ggtgaaaccg ttgggcaaga actggcgggt aacccaaagg tcgcaatggt
      901 cagtatgaca ggcagcgtct ccgctggtga gaagatcatg gcgactgcgg cgaaaaacat
      961 caccaaagtg tgtctggaat tggggggtaa agcaccagct atcgtaatgg acgatgccga
     1021 tcttgaactg gcagtcaaag ccatcgttga ttcacgcgtc attaatagtg ggcaagtgtg
     1081 taactgtgca gaacgtgttt atgtacagaa aggcatttat gatcagttcg tcaatcggct
     1141 gggtgaagcg atgcaggcgg ttcaatttgg taaccccgct gaacgcaacg acattgcgat
     1201 ggggccgttg attaacgccg cggcgctgga aagggtcgag caaaaagtgg cgcgcgcagt
     1261 agaagaaggg gcgagagtgg cgttcggtgg caaagcggtg gaggggaaag gatattatta
     1321 tccgccgaca ttgctgctgg atgttcgcca ggaaatgtcg attatgcatg aggaaacctt
     1381 tggcccggtg ctgccagttg tcgcatttga cacgctggaa gatgctatct caatggctaa
     1441 tgacagtgat tacggcctga cctcatcaat ctatacccaa aatctgaacg tcgcgatgaa
     1501 agccattaaa gggctgaagt ttggtgaaac ttacatcaac cgtgaaaact tcgaagctat
     1561 gcaaggcttc cacgccggat ggcgtaaatc cggtattggc ggcgcagatg gtaaacatgg
     1621 cttgcatgaa tatctccaaa cccaggtggt ttatttacag tcttaatagt actagagcca
     1681 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1741 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1801 tttata
//