BBa_K936014 1 BBa_K936014 pelB-LC-Cutinase with polyhistidine-tag 2012-08-08T11:00:00Z 2015-05-08T01:13:47Z This part was created from other BioBrick parts. pelB leader sequence attached to enzyme LC-Cutinase protein coding sequence in order to allow the cell to bring the enzyme to the periplasm for easier secretion. The polyhistidine-tag allows for us to purify the produced enzyme. false true _1201_ 0 10185 9 Not in stock false We designed the pelB tag to be immediately in front of the LC-cutinase gene. false Nicholas Csicsery annotation2181454 1 pelB range2181454 1 1 66 annotation2181456 1 Polyhistidine-tag range2181456 1 946 963 annotation2181455 1 LC-Cutinase range2181455 1 67 945 BBa_K936020 1 BBa_K936020 K206000+B0034+pelB+Cutinase+Polyhistidine Tag 2012-09-19T11:00:00Z 2015-05-08T01:13:47Z http://www.google.com/url?q=http%3A%2F%2Faem.asm.org%2Fcontent%2F78%2F5%2F1556.long&sa=D&sntz=1&usg=AFQjCNGjFC2TH89mPrYkWCAiYVccsHzdig Released HQ 2013 Full Cutinase Construct on psB1A3 backbone. false false _1201_ 0 13899 9 In stock false There's no terminator so it needs to be used with a backbone that ends with a 3 false Mattan Hamou component2188710 1 BBa_K936015 component2188714 1 BBa_K936014 annotation2188714 1 BBa_K936014 range2188714 1 157 1125 annotation2188710 1 BBa_K936015 range2188710 1 1 150 BBa_K936015 1 BBa_K936015 K206000+B0034 2012-08-08T11:00:00Z 2015-05-08T01:13:47Z Distribution Kit This part is a composite part of the promoter K206000 (pBAD) upstream of the RBS B0034. K206000 is a variant pBAD promoter with a modified AraI1 site that has been shown both to be responsive to lower concentrations of arabinose and to exhibit a higher maximum expression than the wild type. B0034 is a general ribosome binding site. false false _1201_ 0 10185 9 It's complicated false N/A false Nicholas Csicsery annotation2179446 1 BBa_K206000 range2179446 1 1 130 annotation2179448 1 AraI1 range2179448 1 40 57 annotation2179450 1 BBa_B0034 range2179450 1 139 150 annotation2179449 1 AraI2 range2179449 1 61 78 annotation2179447 1 BBa_K936015 range2179447 1 1 150 BBa_K936015_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaa BBa_K936014_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggacggagttctctggcgagtgcgaaccgcggcgctcatggccgcgctgctcgccctcgcagcctgggcgctggtctgggcttcgcccagcgtcgaggctcaatccaacccgtaccagcgcggtcccaaccccacgcggagcgcgctcacggccgacgggccgttctcggtggcaacctacaccgtttcgcggctctcggtgagcggcttcgggggcggggtgatctactaccccacaggcacctcgctgaccttcggcgggatcgccatgtcgccggggtacacggccgacgccagttcgctggcgtggctcgggcggcggttggcatcgcacggcttcgtggtgctcgtcatcaacacgaactcgcgattcgactaccctgactcccgcgcaagccagctatcggcggcgctgaactacctgcggacgagcagcccctcagccgtacgcgcccggctcgatgcgaaccgcctggccgttgcggggcattcgatgggcggcggcgggaccctccgcatcgcagagcagaacccgtcgctgaaagcggccgtaccgctcacgccgtggcacaccgacaagacgttcaacacgtcggtaccggtgctcatcgtgggagcggaagcggacacggtcgcgcccgtgagccagcacgccattccgttctaccagaacttgccctcgaccacgccgaaggtgtacgtggagctcgacaatgcgtcgcacttcgcgcccaacagcaacaacgcggcgatctccgtgtacaccatctcgtggatgaagctgtgggtggataacgacacccgctaccggcagttcctctgcaacgtgaacgatccggcgctgagcgacttccggacgaacaaccgccactgccagcatcaccaccatcaccattagtaa BBa_K936020_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggacggagttctctggcgagtgcgaaccgcggcgctcatggccgcgctgctcgccctcgcagcctgggcgctggtctgggcttcgcccagcgtcgaggctcaatccaacccgtaccagcgcggtcccaaccccacgcggagcgcgctcacggccgacgggccgttctcggtggcaacctacaccgtttcgcggctctcggtgagcggcttcgggggcggggtgatctactaccccacaggcacctcgctgaccttcggcgggatcgccatgtcgccggggtacacggccgacgccagttcgctggcgtggctcgggcggcggttggcatcgcacggcttcgtggtgctcgtcatcaacacgaactcgcgattcgactaccctgactcccgcgcaagccagctatcggcggcgctgaactacctgcggacgagcagcccctcagccgtacgcgcccggctcgatgcgaaccgcctggccgttgcggggcattcgatgggcggcggcgggaccctccgcatcgcagagcagaacccgtcgctgaaagcggccgtaccgctcacgccgtggcacaccgacaagacgttcaacacgtcggtaccggtgctcatcgtgggagcggaagcggacacggtcgcgcccgtgagccagcacgccattccgttctaccagaacttgccctcgaccacgccgaaggtgtacgtggagctcgacaatgcgtcgcacttcgcgcccaacagcaacaacgcggcgatctccgtgtacaccatctcgtggatgaagctgtgggtggataacgacacccgctaccggcagttcctctgcaacgtgaacgatccggcgctgagcgacttccggacgaacaaccgccactgccagcatcaccaccatcaccattagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z