BBa_K936033 1 BBa_K936033 LC Cutinase mutant F125Y 2012-10-02T11:00:00Z 2015-05-08T01:13:48Z Modified from the paper Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach by Sulaiman et al. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294458/ Mutant of the Polyetheylene Terephtalate degrading protein LC Cutinase (Bba_K936000). The phenylalanine at the 125th amino acid position is changed to a tyrosine. This part was created using site directed mutagenesis to change a "TTC" codon to a "TAT" codon. false false _1201_ 0 13882 9 It's complicated false Eliminated BioBrick restriction sites and added a TAA double stop. false Sergio Castro Waqued BBa_K936033_sequence 1 atggacggagttctctggcgagtgcgaaccgcggcgctcatggccgcgctgctcgccctcgcagcctgggcgctggtctgggcttcgcccagcgtcgaggctcaatccaacccgtaccagcgcggtcccaaccccacgcggagcgcgctcacggccgacgggccgttctcggtggcaacctacaccgtttcgcggctctcggtgagcggcttcgggggcggggtgatctactaccccacaggcacctcgctgaccttcggcgggatcgccatgtcgccggggtacacggccgacgccagttcgctggcgtggctcgggcggcggttggcatcgcacggcttcgtggtgctcgtcatcaacacgaactcgcgatatgactaccctgactcccgcgcaagccagctatcggcggcgctgaactacctgcggacgagcagcccctcagccgtacgcgcccggctcgatgcgaaccgcctggccgttgcggggcattcgatgggcggcggcgggaccctccgcatcgcagagcagaacccgtcgctgaaagcggccgtaccgctcacgccgtggcacaccgacaagacgttcaacacgtcggtaccggtgctcatcgtgggagcggaagcggacacggtcgcgcccgtgagccagcacgccattccgttctaccagaacttgccctcgaccacgccgaaggtgtacgtggagctcgacaatgcgtcgcacttcgcgcccaacagcaacaacgcggcgatctccgtgtacaccatctcgtggatgaagctgtgggtggataacgacacccgctaccggcagttcctctgcaacgtgaacgatccggcgctgagcgacttccggacgaacaaccgccactgccagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z