BBa_K944003
1
BBa_K944003
cioA gene related to cyanide insensitivity
2012-09-25T11:00:00Z
2015-05-08T01:13:48Z
This came from the Pseudomonas aeruginosa genomic sequence we searched in genbank and synthesized by IDT Technologies.
cioA encodes a hydrophobic protein of 488 amino acid residues with a predicted molecular mass of 54241 Da.cioA form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases.
false
false
_1211_
0
7118
9
It's complicated
false
This part has no restriction sites used in the protocol standard 10.
false
Natasha G??mez
BBa_K944006
1
BBa_K944006
CioAB genes - Cyanide-Insensitive terminal oxidase
2012-09-25T11:00:00Z
2015-05-08T01:13:48Z
This biobrick is product of a ligation from CioA and CioB.
CioAB has an important role in allowing aerobic respiration under cyanogenic growth conditions, because it encodes the subunits of a terminal oxidase homologous to the cytochrome bd quinol oxidases in bacteria. Under low oxygen tensions, these proteins provide an alternative to electrons transport.
We used this part to give resistance to cyanide to our bacteria under low oxygen tension.
false
true
_1211_
0
7118
9
Not in stock
false
No considerations right now.
false
Natasha G??mez
component2202550
1
BBa_K944004
component2202549
1
BBa_K944003
annotation2202549
1
BBa_K944003
range2202549
1
1
1467
annotation2202550
1
BBa_K944004
range2202550
1
1474
2481
BBa_K944004
1
BBa_K944004
cioB gene related to cyanide insensitivity
2012-09-25T11:00:00Z
2015-05-08T01:13:48Z
This part came from the Pseudomonas aeruginosa genomic sequence and synthesized by IDT Technologies.
cioB encodes a protein, which has the characteristics of a membrane protein, containing 335 amino acids with a predicted molecular mass of 3706 Da. cioB form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases.
false
false
_1211_
0
7118
9
It's complicated
false
The cioB naturaly had a Pst1 restriction site in its sequence so we did a silent mutation for no longer to be this restriction site and proceed with the standard protocol 10.
false
Natasha G??mez
BBa_K944006_sequence
1
atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactgatactagatgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactga
BBa_K944004_sequence
1
atgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactga
BBa_K944003_sequence
1
atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z