BBa_K944010 1 BBa_K944010 Transcriptional regulator of the cyanide inducible promoter and RBS 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This is the ligation of the Transcriptional Regulator from the cyanase operon of Pseudomonas pseudoalcaligenes CECT5344 existing biobricks from the registry. bbThis BioBrick is the ligation of our brand new Transcriptional Regulator (BBa_K944002) and the RBS (BBa_B0034) which is an existing biobrick from the registry. false false _1211_ 0 7118 9 Not in stock false No considerations right now. false Natasha G??mez component2202558 1 BBa_K944002 component2202560 1 BBa_B0034 annotation2202560 1 BBa_B0034 range2202560 1 1941 1952 annotation2202558 1 BBa_K944002 range2202558 1 1 1932 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K944002 1 BBa_K944002 Transcriptional Regulator for Cyanide Inducible Promoter 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This part came from the genomic sequence of Pseudomonas pseudoalcaligenes CECT5344 and was synthesized by IDT technologies. The cynF gene codes for a sigma ?54-dependent transcriptional regulator of 648-amino-acid residues, which belongs to the FIS (factor for inversion stimulation) family of regulatory proteins. P. pseudoalcaligenes CynF shared 59% identity with R. palustris CynF (YP_485671) and 58% identity with Bradyrhizobium japonicum CynF (NP_772375). This Transcriptional regulator works along with the cyanide and cyanide compounds inducible promoter in the natural ocurring cyanase operon from P. pseudoalcaligenes. false false _1211_ 0 7118 9 It's complicated false This part had 3 illegal Pst1 restriction sites. false Natasha G??mez annotation2199373 1 Transcriptional Regulator range2199373 1 1 1932 annotation2199372 1 cynF range2199372 1 1 1932 annotation2199378 1 stop codon range2199378 1 1930 1932 annotation2199377 1 silent mutation range2199377 1 1277 1277 annotation2199376 1 silent mutation range2199376 1 1253 1253 annotation2199374 1 start codon range2199374 1 1 3 annotation2199375 1 silent mutation range2199375 1 890 890 BBa_B0034_sequence 1 aaagaggagaaa BBa_K944010_sequence 1 atgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtagtactagagaaagaggagaaa BBa_K944002_sequence 1 atgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z