BBa_K944006 1 BBa_K944006 CioAB genes - Cyanide-Insensitive terminal oxidase 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This biobrick is product of a ligation from CioA and CioB. CioAB has an important role in allowing aerobic respiration under cyanogenic growth conditions, because it encodes the subunits of a terminal oxidase homologous to the cytochrome bd quinol oxidases in bacteria. Under low oxygen tensions, these proteins provide an alternative to electrons transport. We used this part to give resistance to cyanide to our bacteria under low oxygen tension. false true _1211_ 0 7118 9 Not in stock false No considerations right now. false Natasha G??mez component2202549 1 BBa_K944003 component2202550 1 BBa_K944004 annotation2202550 1 BBa_K944004 range2202550 1 1474 2481 annotation2202549 1 BBa_K944003 range2202549 1 1 1467 BBa_K944003 1 BBa_K944003 cioA gene related to cyanide insensitivity 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This came from the Pseudomonas aeruginosa genomic sequence we searched in genbank and synthesized by IDT Technologies. cioA encodes a hydrophobic protein of 488 amino acid residues with a predicted molecular mass of 54241 Da.cioA form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases. false false _1211_ 0 7118 9 It's complicated false This part has no restriction sites used in the protocol standard 10. false Natasha G??mez BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K944004 1 BBa_K944004 cioB gene related to cyanide insensitivity 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This part came from the Pseudomonas aeruginosa genomic sequence and synthesized by IDT Technologies. cioB encodes a protein, which has the characteristics of a membrane protein, containing 335 amino acids with a predicted molecular mass of 3706 Da. cioB form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases. false false _1211_ 0 7118 9 It's complicated false The cioB naturaly had a Pst1 restriction site in its sequence so we did a silent mutation for no longer to be this restriction site and proceed with the standard protocol 10. false Natasha G??mez BBa_K944008 1 BBa_K944008 GFP and a double terminator 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This is the ligation of two existing biobricks from the registry. Released HQ 2013 For more information about this two parts please visit the page of the BioBricks BBa_E0040 for GFP and BBa_B0015 for the double terminator. false false _1211_ 0 7118 9 In stock false No considerations right now. false Natasha G??mez component2202448 1 BBa_E0040 component2202455 1 BBa_B0015 annotation2202448 1 BBa_E0040 range2202448 1 1 720 annotation2202455 1 BBa_B0015 range2202455 1 729 857 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K944011 1 BBa_K944011 CioAB with GFP Reporter+Ter 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This biobrick is product of a ligation from part BBa_K944006 and BBa_K944008 CioAB genes code for a cyanide-insensitive terminal oxidase that gives bacteria another pathway for the electrons transport. In this part, it is coupled with a reporter gene (GFP) and a terminator. false false _1211_ 0 7118 9 Not in stock false No considerations right now. false Natasha G??mez component2202571 1 BBa_K944006 component2202581 1 BBa_K944008 annotation2202581 1 BBa_K944008 range2202581 1 2488 3344 annotation2202571 1 BBa_K944006 range2202571 1 1 2481 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K944006_sequence 1 atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactgatactagatgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactga BBa_K944008_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K944004_sequence 1 atgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K944011_sequence 1 atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactgatactagatgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactgatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K944003_sequence 1 atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactga BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z