LOCUS       BBa_K944012             2001 bp    DNA     linear       22-Nov-2024
DEFINITION  T7 constitutive promoter with RBS, transcriptional regulator from 
            de cyanide inducible promoter and
ACCESSION   BBa_K944012
VERSION     BBa_K944012.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    1..43
                     /label=BBa_K944009
     misc_feature    50..1981
                     /label=cynF
     misc_feature    50..1981
                     /label=Transcriptional Regulator
     misc_feature    50..52
                     /label=start codon
     misc_feature    939..939
                     /label=silent mutation
     misc_feature    1302..1302
                     /label=silent mutation
     misc_feature    1326..1326
                     /label=silent mutation
     misc_feature    1979..1981
                     /label=stop codon
     promoter        50..1981
                     /label=BBa_K944002
     misc_feature    1994..1997
                     /label=conserved
     RBS             1990..2001
                     /label=BBa_B0034
     misc_feature    50..2001
                     /label=BBa_K944010
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgcccgatcc
       61 caatgccgcc agccagttgt tgctgcaaca tatgcagagc gccagcctgg tcatcgatcc
      121 aatccacgac cgcatcctgc atgccaatcc ggcctgctgc gccctgctcg actggccgct
      181 ggatgaactg ctcgcactgc gcgccagccg tctctgggcg catgaccttg gggcactggt
      241 caccttcacc gaggaagtgc aggtgcgcgg caatgcctgg cgcgacggcc tcgggctgct
      301 gcgtcgcgac ggcgagcgcc tggaagtgga aatcgacggc agccgactgg agcatgacgg
      361 cgtcatactg ctcggcctgc tgatccagca gcgccgccgc ctcgacgccc tgcgcgggct
      421 ggccgaagcc gagcgctatc aacgtcaggg tctgctcgaa tggcagggcg tggaacgcat
      481 cttccgccag ttcgagcggc agaatcagtt gatcctcggc gccgctggcg agggcatcta
      541 cggcgtcaac cgtaatggcg agaccacctt cgtcaacccg gcggccgagc gcattctcgg
      601 ctggcgtgcc gacgatctga tcggccacaa cattcacgac ctgattcacc accatcatcc
      661 ggacggcagc acctacgacg gccacacctg cccgatctac gccgccttca atgatggcga
      721 agtgcatcag gtggccgacg aggtgttctg gaacaaggac ggcaagccga tcccggtgga
      781 ttacaccagc acgccgctgc gcgacgacgg cgaactggtc ggcgccgtgg tggtgtttcg
      841 cgacatcagc gagcgcctgg aaaccgagcg ccggctgcgc caggctctga gcgaggtgca
      901 gcagctcaag caacgcctgg aactggagaa tgcctaccta caggaagaga ttcgtggcga
      961 gtacgcccag cacgacctgg tcgggcgcag cgccgccatg cagcaggtaa tccaccagat
     1021 cgagctggtc gcccccaccg gcgccaacgt gctgatcacc ggcgagtcag gcaccggcaa
     1081 ggagctgatc gcccgcgcca ttcacgacaa cagcgggcgc agtcgtcgcc cgctgatccg
     1141 agtgaactgc gctgcggtgc cgcgcgagct gttcgagagc gagttcttcg gccatatccg
     1201 cggcgccttc accggcgcgc tgaacgaccg cgtcgggcgc ttcgaactgg ccgacggcgg
     1261 cacgctgttt ctcgacgagg tcggcgaaat tcctctggag ctacagagca agctgctgcg
     1321 cgtgctacag gaacagcaac tggaacgggt cggcgacacc cgcacccgtc aggtcgacgt
     1381 acgcgtgatc gccgccacca accgcgacct gcgccaggaa gtacgcgccg gccgcttccg
     1441 cgaagacctg tactttcgcc tcaacgtctt ccccatcgag tcggcgccgc tgcgtcagcg
     1501 cccggaagac attccaccgc tggcccagca ctttctcggc cgcgcctgtc gtcagctcaa
     1561 ccgccctgag ccgagcctgc gcctggccga catccaacgc ctgcaagcct attcctggcc
     1621 gggcaacatt cgtgagctgg agaacctgat cgagcgtgcg gtgatcattt cgcccggcac
     1681 ccggctgcgt ctggatttgc ccaacgacaa cggcaacgac gcaccgtcac ggccaccggt
     1741 cgaacaggaa atgcggattt tcactcaggg tgaacaacgc cggcagatgc gcgaaaacct
     1801 gatcgcggcg ctgacggcct gcgcaggcag gatatcgggg aaggacggcg cggcgcggtt
     1861 gctggagctc aagcccacca ccctacgctc ccgtctggag agcttcgcca tcgacccgcg
     1921 cgactaccga ccggggaggt cgcagccgcg caggtacgag acgaatcaga cgccgaggta
     1981 gtactagaga aagaggagaa a
//