BBa_K944008 1 BBa_K944008 GFP and a double terminator 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This is the ligation of two existing biobricks from the registry. Released HQ 2013 For more information about this two parts please visit the page of the BioBricks BBa_E0040 for GFP and BBa_B0015 for the double terminator. false false _1211_ 0 7118 9 In stock false No considerations right now. false Natasha G??mez component2202455 1 BBa_B0015 component2202448 1 BBa_E0040 annotation2202448 1 BBa_E0040 range2202448 1 1 720 annotation2202455 1 BBa_B0015 range2202455 1 729 857 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K944006 1 BBa_K944006 CioAB genes - Cyanide-Insensitive terminal oxidase 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This biobrick is product of a ligation from CioA and CioB. CioAB has an important role in allowing aerobic respiration under cyanogenic growth conditions, because it encodes the subunits of a terminal oxidase homologous to the cytochrome bd quinol oxidases in bacteria. Under low oxygen tensions, these proteins provide an alternative to electrons transport. We used this part to give resistance to cyanide to our bacteria under low oxygen tension. false true _1211_ 0 7118 9 Not in stock false No considerations right now. false Natasha G??mez component2202550 1 BBa_K944004 component2202549 1 BBa_K944003 annotation2202549 1 BBa_K944003 range2202549 1 1 1467 annotation2202550 1 BBa_K944004 range2202550 1 1474 2481 BBa_K944003 1 BBa_K944003 cioA gene related to cyanide insensitivity 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This came from the Pseudomonas aeruginosa genomic sequence we searched in genbank and synthesized by IDT Technologies. cioA encodes a hydrophobic protein of 488 amino acid residues with a predicted molecular mass of 54241 Da.cioA form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases. false false _1211_ 0 7118 9 It's complicated false This part has no restriction sites used in the protocol standard 10. false Natasha G??mez BBa_K944010 1 BBa_K944010 Transcriptional regulator of the cyanide inducible promoter and RBS 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This is the ligation of the Transcriptional Regulator from the cyanase operon of Pseudomonas pseudoalcaligenes CECT5344 existing biobricks from the registry. bbThis BioBrick is the ligation of our brand new Transcriptional Regulator (BBa_K944002) and the RBS (BBa_B0034) which is an existing biobrick from the registry. false false _1211_ 0 7118 9 Not in stock false No considerations right now. false Natasha G??mez component2202560 1 BBa_B0034 component2202558 1 BBa_K944002 annotation2202558 1 BBa_K944002 range2202558 1 1 1932 annotation2202560 1 BBa_B0034 range2202560 1 1941 1952 BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_K944004 1 BBa_K944004 cioB gene related to cyanide insensitivity 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This part came from the Pseudomonas aeruginosa genomic sequence and synthesized by IDT Technologies. cioB encodes a protein, which has the characteristics of a membrane protein, containing 335 amino acids with a predicted molecular mass of 3706 Da. cioB form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases. false false _1211_ 0 7118 9 It's complicated false The cioB naturaly had a Pst1 restriction site in its sequence so we did a silent mutation for no longer to be this restriction site and proceed with the standard protocol 10. false Natasha G??mez BBa_K944002 1 BBa_K944002 Transcriptional Regulator for Cyanide Inducible Promoter 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This part came from the genomic sequence of Pseudomonas pseudoalcaligenes CECT5344 and was synthesized by IDT technologies. The cynF gene codes for a sigma ?54-dependent transcriptional regulator of 648-amino-acid residues, which belongs to the FIS (factor for inversion stimulation) family of regulatory proteins. P. pseudoalcaligenes CynF shared 59% identity with R. palustris CynF (YP_485671) and 58% identity with Bradyrhizobium japonicum CynF (NP_772375). This Transcriptional regulator works along with the cyanide and cyanide compounds inducible promoter in the natural ocurring cyanase operon from P. pseudoalcaligenes. false false _1211_ 0 7118 9 It's complicated false This part had 3 illegal Pst1 restriction sites. false Natasha G??mez annotation2199372 1 cynF range2199372 1 1 1932 annotation2199373 1 Transcriptional Regulator range2199373 1 1 1932 annotation2199375 1 silent mutation range2199375 1 890 890 annotation2199377 1 silent mutation range2199377 1 1277 1277 annotation2199376 1 silent mutation range2199376 1 1253 1253 annotation2199374 1 start codon range2199374 1 1 3 annotation2199378 1 stop codon range2199378 1 1930 1932 BBa_K944012 1 BBa_K944012 T7 constitutive promoter with RBS, transcriptional regulator from de cyanide inducible promoter and 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z Most of the parts of this intermediate construct are well characterized biobricks from the registry except for the transcriptional regulator which is a brand new biobrick we add to the registry. This BioBrick is the ligation of the biobrick BBa_K944009 and the biobrick BBa_K944010 which is an intermediate part to form our composite part which aims to express the cioAB cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases. false false _1211_ 0 7118 9 Not in stock false No considerations right now. false Natasha G??mez component2202843 1 BBa_K944010 component2202832 1 BBa_K944009 annotation2202832 1 BBa_K944009 range2202832 1 1 43 annotation2202843 1 BBa_K944010 range2202843 1 50 2001 BBa_K944009 1 BBa_K944009 T7 constitutive promoter and RBS 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This is the ligation of two existing biobricks from the registry. For more information about this two parts please visit the page of the BioBricks BBa_I719005 for T7 promoter and BBa_B0034 for the RBS. false false _1211_ 0 7118 9 In stock false No considerations right now. false Natasha G??mez component2202493 1 BBa_I719005 component2202495 1 BBa_B0034 annotation2202495 1 BBa_B0034 range2202495 1 32 43 annotation2202493 1 BBa_I719005 range2202493 1 1 23 BBa_K944011 1 BBa_K944011 CioAB with GFP Reporter+Ter 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This biobrick is product of a ligation from part BBa_K944006 and BBa_K944008 CioAB genes code for a cyanide-insensitive terminal oxidase that gives bacteria another pathway for the electrons transport. In this part, it is coupled with a reporter gene (GFP) and a terminator. false false _1211_ 0 7118 9 Not in stock false No considerations right now. false Natasha G??mez component2202571 1 BBa_K944006 component2202581 1 BBa_K944008 annotation2202581 1 BBa_K944008 range2202581 1 2488 3344 annotation2202571 1 BBa_K944006 range2202571 1 1 2481 BBa_K944013 1 BBa_K944013 Transcriptional Regulator and cioAB expression platform 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z The transcriptional regulator come from Pseudomonas pseudoalcaligenes, the cioAB gene complex come from Pseudomonas aeruginosa and the other genes are well characterized biobricks from the registry. This is the expression platform for our brand new biobricks. We want both the cioAB gene complex to express constitutively so the bacteria, in this case E. coli, can resist to diferent levels of cyanide. We also want the transcriptional regulator to be constitutive because it will interact with the cyanide to further activate activate the inducible promoter false false _1211_ 0 7118 9 Not in stock false We are working very hard with this construct for further characterization. false Natasha G??mez component2202887 1 BBa_K944011 component2202873 1 BBa_K944012 annotation2202873 1 BBa_K944012 range2202873 1 1 2001 annotation2202887 1 BBa_K944011 range2202887 1 2008 5351 BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_B0034_sequence 1 aaagaggagaaa BBa_K944008_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K944013_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaatactagatgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtagtactagagaaagaggagaaatactagatgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactgatactagatgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactgatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K944004_sequence 1 atgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactga BBa_K944012_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaatactagatgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtagtactagagaaagaggagaaa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K944009_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaa BBa_K944006_sequence 1 atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactgatactagatgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactga BBa_K944010_sequence 1 atgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtagtactagagaaagaggagaaa BBa_K944002_sequence 1 atgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtag BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K944011_sequence 1 atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactgatactagatgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactgatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K944003_sequence 1 atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactga BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z