BBa_K944013
type: ComponentDefinition
persistent identity: BBa K944013
display id: BBa_K944013
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional Regulator and cioAB expression platform
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:48Z
mutable provenance: The transcriptional regulator come from Pseudomonas pseudoalcaligenes, the cioAB gene complex come from Pseudomonas aeruginosa and the other genes are well characterized biobricks from the registry.
owned by: james
owned by: myers
mutable description: This is the expression platform for our brand new biobricks. We want both the cioAB gene complex to express constitutively so the bacteria, in this case E. coli, can resist to diferent levels of cyanide. We also want the transcriptional regulator to be constitutive because it will interact with the cyanide to further activate activate the inducible promoter
discontinued: false
dominant: false
group u list: _1211_
m user id: 0
owner id: 7118
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We are working very hard with this construct for further characterization.
star: false
creator: Natasha G??mez
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K944011
component: BBa_K944012
sequence annotation: BBa_K944012
sequence annotation: BBa_K944011
sequence: https://synbiohub.org/public/igem/BBa_K944013_sequence/1

BBa_K944011
type: Component
persistent identity: component2202887
display id: component2202887
version: 1
top level: BBa_K944013
definition: BBa_K944011
access: public

BBa_K944012
type: Component
persistent identity: component2202873
display id: component2202873
version: 1
top level: BBa_K944013
definition: BBa_K944012
access: public

BBa_K944012
type: SequenceAnnotation
persistent identity: annotation2202873
display id: annotation2202873
version: 1
top level: BBa_K944013
location: https://synbiohub.org/public/igem/BBa_K944013/annotation2202873/range2202873/1
component: BBa_K944012

https://synbiohub.org/public/igem/BBa_K944013/annotation2202873/range2202873/1
type: Range
persistent identity: range2202873
display id: range2202873
version: 1
top level: BBa_K944013
direction: direction/reverse
start: 1
end: 2001
orientation: inline

BBa_K944011
type: SequenceAnnotation
persistent identity: annotation2202887
display id: annotation2202887
version: 1
top level: BBa_K944013
location: https://synbiohub.org/public/igem/BBa_K944013/annotation2202887/range2202887/1
component: BBa_K944011

https://synbiohub.org/public/igem/BBa_K944013/annotation2202887/range2202887/1
type: Range
persistent identity: range2202887
display id: range2202887
version: 1
top level: BBa_K944013
direction: direction/reverse
start: 2008
end: 5351
orientation: inline

BBa_K944011
type: ComponentDefinition
persistent identity: BBa K944011
display id: BBa_K944011
version: 1
was generated by: iGEM to SBOL conversion
description: CioAB with GFP Reporter+Ter
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:48Z
mutable provenance: This biobrick is product of a ligation from part BBa_K944006 and BBa_K944008
owned by: james
owned by: myers
mutable description: CioAB genes code for a cyanide-insensitive terminal oxidase that gives bacteria another pathway for the electrons transport. In this part, it is coupled with a reporter gene (GFP) and a terminator.
discontinued: false
dominant: false
group u list: _1211_
m user id: 0
owner id: 7118
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No considerations right now.
star: false
creator: Natasha G??mez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K944008
component: BBa_K944006
sequence annotation: BBa_K944006
sequence annotation: BBa_K944008
sequence: https://synbiohub.org/public/igem/BBa_K944011_sequence/1

BBa_K944008
type: Component
persistent identity: component2202581
display id: component2202581
version: 1
top level: BBa_K944011
definition: BBa_K944008
access: public

BBa_K944006
type: Component
persistent identity: component2202571
display id: component2202571
version: 1
top level: BBa_K944011
definition: BBa_K944006
access: public

BBa_K944006
type: SequenceAnnotation
persistent identity: annotation2202571
display id: annotation2202571
version: 1
top level: BBa_K944011
location: https://synbiohub.org/public/igem/BBa_K944011/annotation2202571/range2202571/1
component: BBa_K944006

https://synbiohub.org/public/igem/BBa_K944011/annotation2202571/range2202571/1
type: Range
persistent identity: range2202571
display id: range2202571
version: 1
top level: BBa_K944011
direction: direction/reverse
start: 1
end: 2481
orientation: inline

BBa_K944008
type: SequenceAnnotation
persistent identity: annotation2202581
display id: annotation2202581
version: 1
top level: BBa_K944011
location: https://synbiohub.org/public/igem/BBa_K944011/annotation2202581/range2202581/1
component: BBa_K944008

https://synbiohub.org/public/igem/BBa_K944011/annotation2202581/range2202581/1
type: Range
persistent identity: range2202581
display id: range2202581
version: 1
top level: BBa_K944011
direction: direction/reverse
start: 2488
end: 3344
orientation: inline

BBa_K944012
type: ComponentDefinition
persistent identity: BBa K944012
display id: BBa_K944012
version: 1
was generated by: iGEM to SBOL conversion
description: T7 constitutive promoter with RBS, transcriptional regulator from de cyanide inducible promoter and
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:48Z
mutable provenance: Most of the parts of this intermediate construct are well characterized biobricks from the registry except for the transcriptional regulator which is a brand new biobrick we add to the registry.
owned by: james
owned by: myers
mutable description: This BioBrick is the ligation of the biobrick BBa_K944009 and the biobrick BBa_K944010 which is an intermediate part to form our composite part which aims to express the cioAB cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases.
discontinued: false
dominant: false
group u list: _1211_
m user id: 0
owner id: 7118
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No considerations right now.
star: false
creator: Natasha G??mez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K944010
component: BBa_K944009
sequence annotation: BBa_K944009
sequence annotation: BBa_K944010
sequence: https://synbiohub.org/public/igem/BBa_K944012_sequence/1

BBa_K944010
type: Component
persistent identity: component2202843
display id: component2202843
version: 1
top level: BBa_K944012
definition: BBa_K944010
access: public

BBa_K944009
type: Component
persistent identity: component2202832
display id: component2202832
version: 1
top level: BBa_K944012
definition: BBa_K944009
access: public

BBa_K944009
type: SequenceAnnotation
persistent identity: annotation2202832
display id: annotation2202832
version: 1
top level: BBa_K944012
location: https://synbiohub.org/public/igem/BBa_K944012/annotation2202832/range2202832/1
component: BBa_K944009

https://synbiohub.org/public/igem/BBa_K944012/annotation2202832/range2202832/1
type: Range
persistent identity: range2202832
display id: range2202832
version: 1
top level: BBa_K944012
direction: direction/reverse
start: 1
end: 43
orientation: inline

BBa_K944010
type: SequenceAnnotation
persistent identity: annotation2202843
display id: annotation2202843
version: 1
top level: BBa_K944012
location: https://synbiohub.org/public/igem/BBa_K944012/annotation2202843/range2202843/1
component: BBa_K944010

https://synbiohub.org/public/igem/BBa_K944012/annotation2202843/range2202843/1
type: Range
persistent identity: range2202843
display id: range2202843
version: 1
top level: BBa_K944012
direction: direction/reverse
start: 50
end: 2001
orientation: inline

BBa_K944008
type: ComponentDefinition
persistent identity: BBa K944008
display id: BBa_K944008
version: 1
was generated by: iGEM to SBOL conversion
description: GFP and a double terminator
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:48Z
mutable provenance: This is the ligation of two existing biobricks from the registry.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: For more information about this two parts please visit the page of the BioBricks BBa_E0040 for GFP and BBa_B0015 for the double terminator.
discontinued: false
dominant: false
group u list: _1211_
m user id: 0
owner id: 7118
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No considerations right now.
star: false
creator: Natasha G??mez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_E0040
component: BBa_B0015
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K944008_sequence/1

BBa_E0040
type: Component
persistent identity: component2202448
display id: component2202448
version: 1
top level: BBa_K944008
definition: GFP
access: public

BBa_B0015
type: Component
persistent identity: component2202455
display id: component2202455
version: 1
top level: BBa_K944008
definition: BBa_B0015
access: public

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2202448
display id: annotation2202448
version: 1
top level: BBa_K944008
location: https://synbiohub.org/public/igem/BBa_K944008/annotation2202448/range2202448/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K944008/annotation2202448/range2202448/1
type: Range
persistent identity: range2202448
display id: range2202448
version: 1
top level: BBa_K944008
direction: direction/reverse
start: 1
end: 720
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2202455
display id: annotation2202455
version: 1
top level: BBa_K944008
location: https://synbiohub.org/public/igem/BBa_K944008/annotation2202455/range2202455/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K944008/annotation2202455/range2202455/1
type: Range
persistent identity: range2202455
display id: range2202455
version: 1
top level: BBa_K944008
direction: direction/reverse
start: 729
end: 857
orientation: inline

BBa_K944009
type: ComponentDefinition
persistent identity: BBa K944009
display id: BBa_K944009
version: 1
was generated by: iGEM to SBOL conversion
description: T7 constitutive promoter and RBS
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:48Z
mutable provenance: This is the ligation of two existing biobricks from the registry.
owned by: james
owned by: myers
mutable description: For more information about this two parts please visit the page of the BioBricks BBa_I719005 for T7 promoter and BBa_B0034 for the RBS.
discontinued: false
dominant: false
group u list: _1211_
m user id: 0
owner id: 7118
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No considerations right now.
star: false
creator: Natasha G??mez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I719005
component: BBa_B0034
sequence annotation: BBa_I719005
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K944009_sequence/1

BBa_I719005
type: Component
persistent identity: component2202493
display id: component2202493
version: 1
top level: BBa_K944009
definition: pT7
access: public

BBa_B0034
type: Component
persistent identity: component2202495
display id: component2202495
version: 1
top level: BBa_K944009
definition: BBa_B0034
access: public

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2202493
display id: annotation2202493
version: 1
top level: BBa_K944009
location: https://synbiohub.org/public/igem/BBa_K944009/annotation2202493/range2202493/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K944009/annotation2202493/range2202493/1
type: Range
persistent identity: range2202493
display id: range2202493
version: 1
top level: BBa_K944009
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2202495
display id: annotation2202495
version: 1
top level: BBa_K944009
location: https://synbiohub.org/public/igem/BBa_K944009/annotation2202495/range2202495/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K944009/annotation2202495/range2202495/1
type: Range
persistent identity: range2202495
display id: range2202495
version: 1
top level: BBa_K944009
direction: direction/reverse
start: 32
end: 43
orientation: inline

BBa_K944006
type: ComponentDefinition
persistent identity: BBa K944006
display id: BBa_K944006
version: 1
was generated by: iGEM to SBOL conversion
description: CioAB genes - Cyanide-Insensitive terminal oxidase
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:48Z
mutable provenance: This biobrick is product of a ligation from CioA and CioB.
owned by: james
owned by: myers
mutable description: CioAB has an important role in allowing aerobic respiration under cyanogenic growth conditions, because it encodes the subunits of a terminal oxidase homologous to the cytochrome bd quinol oxidases in bacteria. Under low oxygen tensions, these proteins provide an alternative to electrons transport. We used this part to give resistance to cyanide to our bacteria under low oxygen tension.
discontinued: false
dominant: true
group u list: _1211_
m user id: 0
owner id: 7118
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No considerations right now.
star: false
creator: Natasha G??mez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K944004
component: BBa_K944003
sequence annotation: BBa_K944003
sequence annotation: BBa_K944004
sequence: https://synbiohub.org/public/igem/BBa_K944006_sequence/1

BBa_K944004
type: Component
persistent identity: component2202550
display id: component2202550
version: 1
top level: BBa_K944006
definition: BBa_K944004
access: public

BBa_K944003
type: Component
persistent identity: component2202549
display id: component2202549
version: 1
top level: BBa_K944006
definition: BBa_K944003
access: public

BBa_K944003
type: SequenceAnnotation
persistent identity: annotation2202549
display id: annotation2202549
version: 1
top level: BBa_K944006
location: https://synbiohub.org/public/igem/BBa_K944006/annotation2202549/range2202549/1
component: BBa_K944003

https://synbiohub.org/public/igem/BBa_K944006/annotation2202549/range2202549/1
type: Range
persistent identity: range2202549
display id: range2202549
version: 1
top level: BBa_K944006
direction: direction/reverse
start: 1
end: 1467
orientation: inline

BBa_K944004
type: SequenceAnnotation
persistent identity: annotation2202550
display id: annotation2202550
version: 1
top level: BBa_K944006
location: https://synbiohub.org/public/igem/BBa_K944006/annotation2202550/range2202550/1
component: BBa_K944004

https://synbiohub.org/public/igem/BBa_K944006/annotation2202550/range2202550/1
type: Range
persistent identity: range2202550
display id: range2202550
version: 1
top level: BBa_K944006
direction: direction/reverse
start: 1474
end: 2481
orientation: inline

BBa_K944010
type: ComponentDefinition
persistent identity: BBa K944010
display id: BBa_K944010
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional regulator of the cyanide inducible promoter and RBS
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:48Z
mutable provenance: This is the ligation of the Transcriptional Regulator from the cyanase operon of Pseudomonas pseudoalcaligenes CECT5344 existing biobricks from the registry.
owned by: james
owned by: myers
mutable description: bbThis BioBrick is the ligation of our brand new Transcriptional Regulator (BBa_K944002) and the RBS (BBa_B0034) which is an existing biobrick from the registry.
discontinued: false
dominant: false
group u list: _1211_
m user id: 0
owner id: 7118
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No considerations right now.
star: false
creator: Natasha G??mez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_K944002
sequence annotation: BBa_K944002
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K944010_sequence/1

BBa_B0034
type: Component
persistent identity: component2202560
display id: component2202560
version: 1
top level: BBa_K944010
definition: BBa_B0034
access: public

BBa_K944002
type: Component
persistent identity: component2202558
display id: component2202558
version: 1
top level: BBa_K944010
definition: BBa_K944002
access: public

BBa_K944002
type: SequenceAnnotation
persistent identity: annotation2202558
display id: annotation2202558
version: 1
top level: BBa_K944010
location: https://synbiohub.org/public/igem/BBa_K944010/annotation2202558/range2202558/1
component: BBa_K944002

https://synbiohub.org/public/igem/BBa_K944010/annotation2202558/range2202558/1
type: Range
persistent identity: range2202558
display id: range2202558
version: 1
top level: BBa_K944010
direction: direction/reverse
start: 1
end: 1932
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2202560
display id: annotation2202560
version: 1
top level: BBa_K944010
location: https://synbiohub.org/public/igem/BBa_K944010/annotation2202560/range2202560/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K944010/annotation2202560/range2202560/1
type: Range
persistent identity: range2202560
display id: range2202560
version: 1
top level: BBa_K944010
direction: direction/reverse
start: 1941
end: 1952
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Promoter
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ---
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Just a T7 Promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: true
creator: Imperial 2007
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

BBa_K944002
type: ComponentDefinition
persistent identity: BBa K944002
display id: BBa_K944002
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional Regulator for Cyanide Inducible Promoter
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:48Z
mutable provenance: This part came from the genomic sequence of Pseudomonas pseudoalcaligenes CECT5344 and was synthesized by IDT technologies.
owned by: james
owned by: myers
mutable description: The cynF gene codes for a sigma ?54-dependent transcriptional regulator of 648-amino-acid residues, which belongs to the FIS (factor for inversion stimulation) family of regulatory proteins. P. pseudoalcaligenes CynF shared 59% identity with R. palustris CynF (YP_485671) and 58% identity with Bradyrhizobium japonicum CynF (NP_772375). This Transcriptional regulator works along with the cyanide and cyanide compounds inducible promoter in the natural ocurring cyanase operon from P. pseudoalcaligenes.
discontinued: false
dominant: false
group u list: _1211_
m user id: 0
owner id: 7118
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part had 3 illegal Pst1 restriction sites.
star: false
creator: Natasha G??mez
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: start codon
sequence annotation: cynF
sequence annotation: Transcriptional Regulator
sequence annotation: silent mutation
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_K944002_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2199374
display id: annotation2199374
version: 1
top level: BBa_K944002
location: https://synbiohub.org/public/igem/BBa_K944002/annotation2199374/range2199374/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K944002/annotation2199374/range2199374/1
type: Range
persistent identity: range2199374
display id: range2199374
version: 1
top level: BBa_K944002
direction: direction/forward
start: 1
end: 3
orientation: inline

cynF
type: SequenceAnnotation
persistent identity: annotation2199372
display id: annotation2199372
version: 1
top level: BBa_K944002
location: https://synbiohub.org/public/igem/BBa_K944002/annotation2199372/range2199372/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K944002/annotation2199372/range2199372/1
type: Range
persistent identity: range2199372
display id: range2199372
version: 1
top level: BBa_K944002
direction: direction/forward
start: 1
end: 1932
orientation: inline

Transcriptional Regulator
type: SequenceAnnotation
persistent identity: annotation2199373
display id: annotation2199373
version: 1
top level: BBa_K944002
location: https://synbiohub.org/public/igem/BBa_K944002/annotation2199373/range2199373/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K944002/annotation2199373/range2199373/1
type: Range
persistent identity: range2199373
display id: range2199373
version: 1
top level: BBa_K944002
direction: direction/forward
start: 1
end: 1932
orientation: inline

silent mutation
type: SequenceAnnotation
persistent identity: annotation2199375
display id: annotation2199375
version: 1
top level: BBa_K944002
location: https://synbiohub.org/public/igem/BBa_K944002/annotation2199375/range2199375/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K944002/annotation2199375/range2199375/1
type: Range
persistent identity: range2199375
display id: range2199375
version: 1
top level: BBa_K944002
direction: direction/forward
start: 890
end: 890
orientation: inline

silent mutation
type: SequenceAnnotation
persistent identity: annotation2199376
display id: annotation2199376
version: 1
top level: BBa_K944002
location: https://synbiohub.org/public/igem/BBa_K944002/annotation2199376/range2199376/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K944002/annotation2199376/range2199376/1
type: Range
persistent identity: range2199376
display id: range2199376
version: 1
top level: BBa_K944002
direction: direction/forward
start: 1253
end: 1253
orientation: inline

silent mutation
type: SequenceAnnotation
persistent identity: annotation2199377
display id: annotation2199377
version: 1
top level: BBa_K944002
location: https://synbiohub.org/public/igem/BBa_K944002/annotation2199377/range2199377/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K944002/annotation2199377/range2199377/1
type: Range
persistent identity: range2199377
display id: range2199377
version: 1
top level: BBa_K944002
direction: direction/forward
start: 1277
end: 1277
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2199378
display id: annotation2199378
version: 1
top level: BBa_K944002
location: https://synbiohub.org/public/igem/BBa_K944002/annotation2199378/range2199378/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K944002/annotation2199378/range2199378/1
type: Range
persistent identity: range2199378
display id: range2199378
version: 1
top level: BBa_K944002
direction: direction/forward
start: 1930
end: 1932
orientation: inline

BBa_K944003
type: ComponentDefinition
persistent identity: BBa K944003
display id: BBa_K944003
version: 1
was generated by: iGEM to SBOL conversion
description: cioA gene related to cyanide insensitivity
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:48Z
mutable provenance: This came from the Pseudomonas aeruginosa genomic sequence we searched in genbank and synthesized by IDT Technologies.
owned by: james
owned by: myers
mutable description: cioA encodes a hydrophobic protein of 488 amino acid residues with a predicted molecular mass of 54241 Da.cioA form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases.
discontinued: false
dominant: false
group u list: _1211_
m user id: 0
owner id: 7118
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part has no restriction sites used in the protocol standard 10.
star: false
creator: Natasha G??mez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K944003_sequence/1

BBa_K944004
type: ComponentDefinition
persistent identity: BBa K944004
display id: BBa_K944004
version: 1
was generated by: iGEM to SBOL conversion
description: cioB gene related to cyanide insensitivity
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:48Z
mutable provenance: This part came from the Pseudomonas aeruginosa genomic sequence and synthesized by IDT Technologies.
owned by: james
owned by: myers
mutable description: cioB encodes a protein, which has the characteristics of a membrane protein, containing 335 amino acids with a predicted molecular mass of 3706 Da. cioB form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases.
discontinued: false
dominant: false
group u list: _1211_
m user id: 0
owner id: 7118
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The cioB naturaly had a Pst1 restriction site in its sequence so we did a silent mutation for no longer to be this restriction site and proceed with the standard protocol 10.
star: false
creator: Natasha G??mez
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K944004_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K944013_sequence/1
type: Sequence
persistent identity: BBa K944013 sequence
display id: BBa_K944013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtagtactagagaaagaggagaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K944011_sequence/1
type: Sequence
persistent identity: BBa K944011 sequence
display id: BBa_K944011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactgatactagatgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K944012_sequence/1
type: Sequence
persistent identity: BBa K944012 sequence
display id: BBa_K944012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtagtactagagaaagaggagaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K944008_sequence/1
type: Sequence
persistent identity: BBa K944008 sequence
display id: BBa_K944008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K944009_sequence/1
type: Sequence
persistent identity: BBa K944009 sequence
display id: BBa_K944009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K944006_sequence/1
type: Sequence
persistent identity: BBa K944006 sequence
display id: BBa_K944006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactgatactagatgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K944010_sequence/1
type: Sequence
persistent identity: BBa K944010 sequence
display id: BBa_K944010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtagtactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I719005_sequence/1
type: Sequence
persistent identity: BBa I719005 sequence
display id: BBa_I719005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K944002_sequence/1
type: Sequence
persistent identity: BBa K944002 sequence
display id: BBa_K944002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K944003_sequence/1
type: Sequence
persistent identity: BBa K944003 sequence
display id: BBa_K944003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K944004_sequence/1
type: Sequence
persistent identity: BBa K944004 sequence
display id: BBa_K944004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z