LOCUS       BBa_K944014             1139 bp    DNA     linear       26-Dec-2024
DEFINITION  Cyanide Biosensor expression platform using a cyanide inducible 
            promoter
ACCESSION   BBa_K944014
VERSION     BBa_K944014.1  
FEATURES             Location/Qualifiers
     promoter        1..270
                     /label=BBa_K944000
     misc_feature    283..286
                     /label=conserved
     RBS             279..290
                     /label=BBa_B0034
     misc_feature    1..290
                     /label=BBa_K944005
     CDS             297..971
                     /label=mrfp1
     misc_feature    978..1002
                     /label=Help:Barcodes
     CDS             297..1002
                     /label=BBa_E1010
     stem_loop       1022..1065
                     /label=stem_loop
     misc_feature    1011..1090
                     /label=BBa_B0010
     terminator      1011..1090
                     /label=BBa_B0010
     stem_loop       1106..1125
                     /label=T7 TE
     misc_feature    1132..1132
                     /label=stop
     polyA_site      1126..1139
                     /label=polya
     misc_feature    1099..1139
                     /label=BBa_B0012
     terminator      1099..1139
                     /label=BBa_B0012
     terminator      1011..1139
                     /label=BBa_B0015
     misc_feature    297..1139
                     /label=BBa_K944007
ORIGIN
        1 tcgtgatcac tcctgcgatg gcctgcccca aattggtgag gcgggcgctg actgggctgg
       61 gccattgctg accggttcat gtcagctatt gcaggagctg taccagttca aaaatctata
      121 taagcgattg aaaaatataa acttttaaca aaaccaataa atacgaaatc tcgttattca
      181 cgagatttcg tcatcttgaa aaacgagata tcgtcaactg gcacgccctt tggataacgt
      241 cctgaaaggt caatcccgag agcgccgcaa tactagagaa agaggagaaa tactagatgg
      301 cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg
      361 ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc
      421 agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt
      481 ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact
      541 acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg
      601 gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag
      661 ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg
      721 gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca
      781 aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca
      841 tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca
      901 cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact
      961 ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca
     1021 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1081 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//