LOCUS BBa_K944015 6498 bp DNA linear 23-Dec-2024 DEFINITION Cyanide insensitive terminal oxidase cioAB and cyanide biosensor expression platform ACCESSION BBa_K944015 VERSION BBa_K944015.1 FEATURES Location/Qualifiers promoter 1..23 /label=BBa_I719005 misc_feature 36..39 /label=conserved RBS 32..43 /label=BBa_B0034 misc_feature 1..43 /label=BBa_K944009 misc_feature 50..1981 /label=cynF misc_feature 50..1981 /label=Transcriptional Regulator misc_feature 50..52 /label=start codon misc_feature 939..939 /label=silent mutation misc_feature 1302..1302 /label=silent mutation misc_feature 1326..1326 /label=silent mutation misc_feature 1979..1981 /label=stop codon promoter 50..1981 /label=BBa_K944002 misc_feature 1994..1997 /label=conserved RBS 1990..2001 /label=BBa_B0034 misc_feature 50..2001 /label=BBa_K944010 misc_feature 1..2001 /label=BBa_K944012 CDS 2008..3474 /label=BBa_K944003 CDS 3481..4488 /label=BBa_K944004 misc_feature 2008..4488 /label=BBa_K944006 CDS 4495..5214 /label=GFP protein CDS 4495..5214 /label=BBa_E0040 stem_loop 5234..5277 /label=stem_loop misc_feature 5223..5302 /label=BBa_B0010 terminator 5223..5302 /label=BBa_B0010 stem_loop 5318..5337 /label=T7 TE misc_feature 5344..5344 /label=stop polyA_site 5338..5351 /label=polya misc_feature 5311..5351 /label=BBa_B0012 terminator 5311..5351 /label=BBa_B0012 terminator 5223..5351 /label=BBa_B0015 misc_feature 4495..5351 /label=BBa_K944008 misc_feature 2008..5351 /label=BBa_K944011 misc_feature 1..5351 /label=BBa_K944013 promoter 5360..5629 /label=BBa_K944000 misc_feature 5642..5645 /label=conserved RBS 5638..5649 /label=BBa_B0034 misc_feature 5360..5649 /label=BBa_K944005 CDS 5656..6330 /label=mrfp1 misc_feature 6337..6361 /label=Help:Barcodes CDS 5656..6361 /label=BBa_E1010 stem_loop 6381..6424 /label=stem_loop misc_feature 6370..6449 /label=BBa_B0010 terminator 6370..6449 /label=BBa_B0010 stem_loop 6465..6484 /label=T7 TE misc_feature 6491..6491 /label=stop polyA_site 6485..6498 /label=polya misc_feature 6458..6498 /label=BBa_B0012 terminator 6458..6498 /label=BBa_B0012 terminator 6370..6498 /label=BBa_B0015 misc_feature 5656..6498 /label=BBa_K944007 misc_feature 5360..6498 /label=BBa_K944014 ORIGIN 1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgcccgatcc 61 caatgccgcc agccagttgt tgctgcaaca tatgcagagc gccagcctgg tcatcgatcc 121 aatccacgac cgcatcctgc atgccaatcc ggcctgctgc gccctgctcg actggccgct 181 ggatgaactg ctcgcactgc gcgccagccg tctctgggcg catgaccttg gggcactggt 241 caccttcacc gaggaagtgc aggtgcgcgg caatgcctgg cgcgacggcc tcgggctgct 301 gcgtcgcgac ggcgagcgcc tggaagtgga aatcgacggc agccgactgg agcatgacgg 361 cgtcatactg ctcggcctgc tgatccagca gcgccgccgc ctcgacgccc tgcgcgggct 421 ggccgaagcc gagcgctatc aacgtcaggg tctgctcgaa tggcagggcg tggaacgcat 481 cttccgccag ttcgagcggc agaatcagtt gatcctcggc gccgctggcg agggcatcta 541 cggcgtcaac cgtaatggcg agaccacctt cgtcaacccg gcggccgagc gcattctcgg 601 ctggcgtgcc gacgatctga tcggccacaa cattcacgac ctgattcacc accatcatcc 661 ggacggcagc acctacgacg gccacacctg cccgatctac gccgccttca atgatggcga 721 agtgcatcag gtggccgacg aggtgttctg gaacaaggac ggcaagccga tcccggtgga 781 ttacaccagc acgccgctgc gcgacgacgg cgaactggtc ggcgccgtgg tggtgtttcg 841 cgacatcagc gagcgcctgg aaaccgagcg ccggctgcgc caggctctga gcgaggtgca 901 gcagctcaag caacgcctgg aactggagaa tgcctaccta caggaagaga ttcgtggcga 961 gtacgcccag cacgacctgg tcgggcgcag cgccgccatg cagcaggtaa tccaccagat 1021 cgagctggtc gcccccaccg gcgccaacgt gctgatcacc ggcgagtcag gcaccggcaa 1081 ggagctgatc gcccgcgcca ttcacgacaa cagcgggcgc agtcgtcgcc cgctgatccg 1141 agtgaactgc gctgcggtgc cgcgcgagct gttcgagagc gagttcttcg gccatatccg 1201 cggcgccttc accggcgcgc tgaacgaccg cgtcgggcgc ttcgaactgg ccgacggcgg 1261 cacgctgttt ctcgacgagg tcggcgaaat tcctctggag ctacagagca agctgctgcg 1321 cgtgctacag gaacagcaac tggaacgggt cggcgacacc cgcacccgtc aggtcgacgt 1381 acgcgtgatc gccgccacca accgcgacct gcgccaggaa gtacgcgccg gccgcttccg 1441 cgaagacctg tactttcgcc tcaacgtctt ccccatcgag tcggcgccgc tgcgtcagcg 1501 cccggaagac attccaccgc tggcccagca ctttctcggc cgcgcctgtc gtcagctcaa 1561 ccgccctgag ccgagcctgc gcctggccga catccaacgc ctgcaagcct attcctggcc 1621 gggcaacatt cgtgagctgg agaacctgat cgagcgtgcg gtgatcattt cgcccggcac 1681 ccggctgcgt ctggatttgc ccaacgacaa cggcaacgac gcaccgtcac ggccaccggt 1741 cgaacaggaa atgcggattt tcactcaggg tgaacaacgc cggcagatgc gcgaaaacct 1801 gatcgcggcg ctgacggcct gcgcaggcag gatatcgggg aaggacggcg cggcgcggtt 1861 gctggagctc aagcccacca ccctacgctc ccgtctggag agcttcgcca tcgacccgcg 1921 cgactaccga ccggggaggt cgcagccgcg caggtacgag acgaatcaga cgccgaggta 1981 gtactagaga aagaggagaa atactagatg ttcggattgg aggctatcga ccttgcccgc 2041 atccagttcg cgttcaccgt gtccttccac atcatcttcc cggcgatcac catcgggttg 2101 gccagctacc tggccgtact ggaaggcctc tggctgaaaa cgaacgaaga ggtctaccgc 2161 gacctctatc atttctggtc gaagattttc gcggtcaact tcggcatggg cgtggtgtcc 2221 gggctggtca tggcgtacca gttcggcacc aactggagcc atttctccga cttcgccggg 2281 tccatcaccg gtccgctgct gacctacgag gtgctcaccg cgttcttcct cgaggccggt 2341 ttcctcgggg tcatgctgtt cggctggaac cgcgtgggac cgggcctgca tttcttcgcc 2401 acggtgatgg tggcgatcgg cacgctgatc tcgacgttct ggatcctcgc ttccaacagc 2461 tggatgcaga ccccgcaggg tttcgagatc gtcgacggcc gggtgatccc ggtggactgg 2521 ctggcggtga tcttcaaccc gtcgttcccc taccgcctgc tgcacatgtc ggtcgccgcg 2581 ttcctcgcca ccgccttctt cgtcggcgcc tcggccgcct ggcacctgct gcgcgggcgc 2641 gacaacccgg cgatccgcaa gatgctctcg atggccatgt ggatggcgct gatcgtcgcc 2701 ccggtacagg cgctgatcgg cgacgcccat ggcctcaata ccctggagca ccagccggcg 2761 aagatcgccg cgatggaagg ccactgggac aacagcagcg gcgagccgac cccgctgatc 2821 ctgttcggct ggccggacat gcagcgcgag gaaacccgct tcaaggtgga gatcccggtg 2881 ctcggcagcc tgatcctcaa gcacagcctg accgagccga tcccggcgct gaaggacttc 2941 ccgccggaag accggccgaa ctccaccgtg gtgttctggt cgttccggat catggccggc 3001 ctcggcatgc tgatgatcct ggtcggcgtc tggagcctgt ggctgcgctg gcgcggccag 3061 gacaagctgt tcaactcgaa ggccttcctg cgcctgaccc tgctgatggg accgtccggc 3121 ctgatcgcga tcctcgccgg ctggttcacc accgagatgg gccgccagcc ctgggtggtc 3181 tacggactga tgcgcaccgc cgacgcctcc tcggcgcaga gcgtgacgca gatgagcctg 3241 accctgctga ccttcgtggt ggtgtacttc tcgctgttcg gcgtcggtat cggctacatg 3301 atgcgcctgg tgcgcaaggg cccggtcacc catgagggac gcgagaccaa cccgggaggc 3361 gccgggcaga agcgcacgcc ggcacggccg ttgtcggccg ccggcgaagg tttcgacgaa 3421 gagcacgacg gccacgcgca agcggccgac cgcgaccagg acaagaggaa ctgatactag 3481 atgggtatcg atcttcccct catctgggcg gtcatcatca tcttcggcat catgatgtac 3541 gtggtgatgg acggcttcga cctgggcatc ggcatgctct atccgttctt caaggacagc 3601 ggcgaccgcg atgtgatgat gaacaccgtg gcgccggtct gggacggcaa cgagacctgg 3661 ctggtgctgg gcggcgcggc gttgttcggg gccttcccgc tggcctacgc ggtggtcctg 3721 tcggcgctct acctgccgtt gatcttcatg ctcgtcgggc tgatcttccg cggcgtcgcc 3781 ttcgagttcc gcttcaaggc caagccggcc aagcagcaca tctgggacaa ggcgttcatc 3841 ggcggttcgc tggtggcgac cttcttccag ggcgtggcgc tgggcgcctt catcgaaggc 3901 atcccggtgg agaaccgcag cttcgccggc ggttcgctgg actggatcgc gccgttcccg 3961 ctgttctgcg gcctggccct gatcgtcgcc tacaccctgc tcggctgcac ctggctgatc 4021 atgaagaccg aaggccgcct acaggagcgc atgcacgacc tggcgcgccc gctggcgttg 4081 gtgctgctgg cgatcatcgg ggtggtcagc ctgtggacgc cgctggccca tgccgagatc 4141 gccgcacgct ggttcagcct gccgaacctg ttctggttcc tgccggtgcc gatcctggtc 4201 ctggtgacct tctatgcgct gctgcgctcg gtggccaaca acgaccacgt gaagccgttc 4261 gtgctcaccc tggtgttgat cttcctcggc tacagcggcc tggggatcag cctgtggccg 4321 aacatcatcc cgccggcggt gagcatctgg gaggcctcgg cgccgccgca gagccagggc 4381 ttcatgctgg tcggcgcgct gttcatcatt cccttcatcc tcggctacac cgcctggagc 4441 tactacgtgt tccgcggcaa ggtgaagcac ggcgatggct atcactgata ctagatgcgt 4501 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 4561 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 4621 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 4681 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 4741 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 4801 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 4861 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 4921 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 4981 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 5041 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 5101 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 5161 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag 5221 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 5281 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 5341 ctgcgtttat atactagagt cgtgatcact cctgcgatgg cctgccccaa attggtgagg 5401 cgggcgctga ctgggctggg ccattgctga ccggttcatg tcagctattg caggagctgt 5461 accagttcaa aaatctatat aagcgattga aaaatataaa cttttaacaa aaccaataaa 5521 tacgaaatct cgttattcac gagatttcgt catcttgaaa aacgagatat cgtcaactgg 5581 cacgcccttt ggataacgtc ctgaaaggtc aatcccgaga gcgccgcaat actagagaaa 5641 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa 5701 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt 5761 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg 5821 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac 5881 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt 5941 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac 6001 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt 6061 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt 6121 gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct 6181 gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc 6241 gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa 6301 cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg 6361 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 6421 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 6481 ggcctttctg cgtttata //