##gff-version 3
##sequence-region BBa_K944015 1 6498
BBa_K944015	.	engineered_region	5360	6498	.	+	0	ID=BBa_K944014;Name=BBa_K944014
BBa_K944015	.	engineered_region	5656	6498	.	+	0	ID=BBa_K944007;Name=BBa_K944007;Parent=BBa_K944014
BBa_K944015	.	terminator	6370	6498	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K944007
BBa_K944015	.	terminator	6370	6449	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K944015	.	stem_loop	6381	6424	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K944015	.	engineered_region	6370	6449	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K944015	.	terminator	6458	6498	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K944015	.	stem_loop	6465	6484	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K944015	.	stop_codon	6491	6491	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K944015	.	engineered_region	6458	6498	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K944015	.	polyA_site	6485	6498	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K944015	.	CDS	5656	6361	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K944007
BBa_K944015	.	engineered_tag	6337	6361	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K944015	.	CDS	5656	6330	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K944015	.	engineered_region	5360	5649	.	+	0	ID=BBa_K944005;Name=BBa_K944005;Parent=BBa_K944014
BBa_K944015	.	ribosome_entry_site	5638	5649	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K944005
BBa_K944015	.	conserved_region	5642	5645	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K944015	.	promoter	5360	5629	.	+	0	ID=BBa_K944000;Name=BBa_K944000;Parent=BBa_K944005
BBa_K944015	.	engineered_region	1	5351	.	+	0	ID=BBa_K944013;Name=BBa_K944013
BBa_K944015	.	engineered_region	1	2001	.	+	0	ID=BBa_K944012;Name=BBa_K944012;Parent=BBa_K944013
BBa_K944015	.	engineered_region	50	2001	.	+	0	ID=BBa_K944010;Name=BBa_K944010;Parent=BBa_K944012
BBa_K944015	.	promoter	50	1981	.	+	0	ID=BBa_K944002;Name=BBa_K944002;Parent=BBa_K944010
BBa_K944015	.	sequence_feature	50	1981	.	+	0	ID=annotation2199372;Name=cynF;Parent=BBa_K944002
BBa_K944015	.	point_mutation	1326	1326	.	+	0	ID=annotation2199377;Name=silent mutation;Parent=BBa_K944002
BBa_K944015	.	start_codon	50	52	.	+	0	ID=annotation2199374;Name=start codon;Parent=BBa_K944002
BBa_K944015	.	engineered_region	50	1981	.	+	0	ID=annotation2199373;Name=Transcriptional Regulator;Parent=BBa_K944002
BBa_K944015	.	point_mutation	939	939	.	+	0	ID=annotation2199375;Name=silent mutation;Parent=BBa_K944002
BBa_K944015	.	stop_codon	1979	1981	.	+	0	ID=annotation2199378;Name=stop codon;Parent=BBa_K944002
BBa_K944015	.	point_mutation	1302	1302	.	+	0	ID=annotation2199376;Name=silent mutation;Parent=BBa_K944002
BBa_K944015	.	ribosome_entry_site	1990	2001	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K944010
BBa_K944015	.	conserved_region	1994	1997	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K944015	.	engineered_region	1	43	.	+	0	ID=BBa_K944009;Name=BBa_K944009;Parent=BBa_K944012
BBa_K944015	.	promoter	1	23	.	+	0	ID=BBa_I719005;Name=BBa_I719005;Parent=BBa_K944009
BBa_K944015	.	ribosome_entry_site	32	43	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K944009
BBa_K944015	.	conserved_region	36	39	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K944015	.	engineered_region	2008	5351	.	+	0	ID=BBa_K944011;Name=BBa_K944011;Parent=BBa_K944013
BBa_K944015	.	engineered_region	2008	4488	.	+	0	ID=BBa_K944006;Name=BBa_K944006;Parent=BBa_K944011
BBa_K944015	.	CDS	3481	4488	.	+	0	ID=BBa_K944004;Name=BBa_K944004;Parent=BBa_K944006
BBa_K944015	.	CDS	2008	3474	.	+	0	ID=BBa_K944003;Name=BBa_K944003;Parent=BBa_K944006
BBa_K944015	.	engineered_region	4495	5351	.	+	0	ID=BBa_K944008;Name=BBa_K944008;Parent=BBa_K944011
BBa_K944015	.	terminator	5223	5351	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K944008
BBa_K944015	.	terminator	5223	5302	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K944015	.	stem_loop	5234	5277	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K944015	.	engineered_region	5223	5302	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K944015	.	terminator	5311	5351	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K944015	.	stem_loop	5318	5337	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K944015	.	stop_codon	5344	5344	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K944015	.	engineered_region	5311	5351	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K944015	.	polyA_site	5338	5351	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K944015	.	CDS	4495	5214	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K944008
BBa_K944015	.	CDS	4495	5214	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K944015
taatacgactcactatagggagatactagagaaagaggagaaatactagatgcccgatcccaatgccgccagccagttg
ttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgct
gcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcact
ggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgag
cgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgcc
gccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtgga
acgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgt
aatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaaca
ttcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaa
tgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagc
acgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagc
gccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaaga
gattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctg
gtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacg
acaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagtt
cttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacg
ctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactgg
aacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagt
acgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgc
ccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgc
gcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgc
ggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccg
gtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacgg
cctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtct
ggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccg
aggtagtactagagaaagaggagaaatactagatgttcggattggaggctatcgaccttgcccgcatccagttcgcgtt
caccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctgg
ctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcg
tggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccgg
tccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaac
cgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcg
cttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggt
gatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggc
gcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatgg
cgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagat
cgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcag
cgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcc
cggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcct
cggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaag
gccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgg
gccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcct
gaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaag
ggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcgg
ccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactgata
ctagatgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggc
ttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgc
cggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggt
ggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgc
ttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagg
gcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgc
gccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagacc
gaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtca
gcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggt
gccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctc
accctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagca
tctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcgg
ctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactgatactagatgcgtaaa
ggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtca
gtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcc
atggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgac
tttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacac
gtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaa
cattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatc
aaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaa
ttggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaa
gagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcgtgatcactcc
tgcgatggcctgccccaaattggtgaggcgggcgctgactgggctgggccattgctgaccggttcatgtcagctattgc
aggagctgtaccagttcaaaaatctatataagcgattgaaaaatataaacttttaacaaaaccaataaatacgaaatct
cgttattcacgagatttcgtcatcttgaaaaacgagatatcgtcaactggcacgccctttggataacgtcctgaaaggt
caatcccgagagcgccgcaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttc
atgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgt
acgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgca
gttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggt
ttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtg
agttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttg
ggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagac
ggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccg
acatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactc
caccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttata