LOCUS       BBa_K945004             1321 bp    DNA     linear       22-Nov-2024
DEFINITION  Pichia pastoris optimized His-GFP
ACCESSION   BBa_K945004
VERSION     BBa_K945004.1  
FEATURES             Location/Qualifiers
     CDS             216..950
                     /label=wt-GFP
     misc_feature    951..1291
                     /label=AOX1 Terminator
     misc_feature    219..236
                     /label=6xHis-tag
     RBS             204..215
                     /label=Yeast RBS
ORIGIN
        1 atgcattcgc gaggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg
       61 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      121 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      181 cgaatggcgt actgtcgccc gggcccgccg ccaccatgca ccaccaccat catcattcca
      241 agggagaaga actttttaca ggagttgtcc caatccttgt tgaacttgac ggagacgtta
      301 acggacaaaa attttctgtt tccggtgaag gagagggaga tgccacatac ggaaagttga
      361 cccttaaatt catttgtact actggtaaat tgcctgttcc ttggccaaca cttgtcacca
      421 ctttttccta cggtgtccaa tgcttctcaa gatatcctga tcatatgaag cagcacgact
      481 ttttcaaatc cgctatgcca gaaggatatg ttcaggagag aactattttc tacaaggatg
      541 acggtaacta caagactaga gccgaggtta agttcgaggg agatacattg gtcaacagaa
      601 tcgaattgaa gggtatcgat ttcaaggagg acggaaatat cttgggtcat aagatggaat
      661 acaactacaa cagtcacaac gtttacatta tggcagacaa gcctaaaaac ggaatcaagg
      721 tcaacttcaa gatcagacat aatatcaagg atggttcagt tcaattggct gaccactacc
      781 aacagaacac ccctattgga gatggtcctg ttttgcttcc agacaatcat tatttgtcaa
      841 ctcagtctgc tttgtctaaa gatccaaacg agaaaagaga ccacatgatc cttttggagt
      901 ttgttactgc cgctggtatc acacacggta tggatgagtt gtataaatag gttttagcct
      961 tagacatgac tgttcctcag ttcaagttgg gcacttacga gaagaccggt cttgctagat
     1021 tctaatcaag aggatgtcag aatgccattt gcctgagaga tgcaggcttc atttttgata
     1081 cttttttatt tgtaacctat atagtatagg attttttttg tcattttgtt tcttctcgta
     1141 cgagcttgct cctgatcagc ctatctcgca gctgatgaat atcttgtggt aggggtttgg
     1201 gaaaatcatt cgagtttgat gtttttcttg gtatttccca ctcctcttca gagtacagaa
     1261 gattaagtga gaccttcgtt tgtgcggatc ccgacccggg cactcgcgac ccaatctaga
     1321 g
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