BBa_K945004 1 BBa_K945004 Pichia pastoris optimized His-GFP 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z The sequence, previous to its modification, came from the optimization of the wild-type GFP found in <i>Aequorea victoria</i>. This sequence codes for a green fluorescent protein (GFP) which has been codon optimized for <i>Pichia pastoris</i> and engineered to include a 6xHis-tag in its N-terminus. false false _1212_ 0 13149 9 It's complicated false The codon optimization utilized had to take in account the illegal restriction sites to be avoided in order for it to be standardized. false Daniel Villalobos Ram??rez annotation2204783 1 AOX1 Terminator range2204783 1 951 1291 annotation2204788 1 Yeast RBS range2204788 1 204 215 annotation2203337 1 wt-GFP range2203337 1 216 950 annotation2204784 1 6xHis-tag range2204784 1 219 236 BBa_K945004_sequence 1 atgcattcgcgaggtaccgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgtactgtcgcccgggcccgccgccaccatgcaccaccaccatcatcattccaagggagaagaactttttacaggagttgtcccaatccttgttgaacttgacggagacgttaacggacaaaaattttctgtttccggtgaaggagagggagatgccacatacggaaagttgacccttaaattcatttgtactactggtaaattgcctgttccttggccaacacttgtcaccactttttcctacggtgtccaatgcttctcaagatatcctgatcatatgaagcagcacgactttttcaaatccgctatgccagaaggatatgttcaggagagaactattttctacaaggatgacggtaactacaagactagagccgaggttaagttcgagggagatacattggtcaacagaatcgaattgaagggtatcgatttcaaggaggacggaaatatcttgggtcataagatggaatacaactacaacagtcacaacgtttacattatggcagacaagcctaaaaacggaatcaaggtcaacttcaagatcagacataatatcaaggatggttcagttcaattggctgaccactaccaacagaacacccctattggagatggtcctgttttgcttccagacaatcattatttgtcaactcagtctgctttgtctaaagatccaaacgagaaaagagaccacatgatccttttggagtttgttactgccgctggtatcacacacggtatggatgagttgtataaataggttttagccttagacatgactgttcctcagttcaagttgggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatttgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtataggattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgcagctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttcttggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggatcccgacccgggcactcgcgacccaatctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z