LOCUS       BBa_K945005             1150 bp    DNA     linear       15-May-2024
DEFINITION  Pichia pastoris optimized His-GFP-PTS1
ACCESSION   BBa_K945005
VERSION     BBa_K945005.1  
FEATURES             Location/Qualifiers
     CDS             39..776
                     /label=wt-GFP
     misc_feature    777..1117
                     /label=AOX1 terminator
     misc_feature    42..59
                     /label=6xHis-tag
     misc_feature    765..773
                     /label=PTS1
     RBS             26..38
                     /label=Yeast RBS
ORIGIN
        1 attcgcgacc cgggcactcg gccggccccg ccgccaccat gcaccatcat catcatcatt
       61 ctaaaggaga ggagttgttc acaggagtcg ttccaattct tgttgagctt gacggagacg
      121 ttaatggaca aaaattttct gtttccggtg aaggagaggg agatgccaca tacggaaagt
      181 tgacccttaa attcatttgt actactggta aattgcctgt tccttggcca acacttgtca
      241 ccactttttc ctacggtgtc caatgcttct caagatatcc tgatcatatg aagcagcacg
      301 actttttcaa atccgctatg ccagaaggat atgttcagga gagaactatt ttctacaagg
      361 atgacggtaa ctacaagact agagccgagg ttaagttcga gggagataca ttggtcaaca
      421 gaatcgaatt gaagggtatc gatttcaagg aggacggaaa tatcttgggt cataagatgg
      481 aatacaacta caacagtcac aacgtttaca ttatggcaga caagcctaaa aacggaatca
      541 aggtcaactt caagatcaga cataatatca aggatggttc agttcaattg gctgaccact
      601 accaacagaa cacccctatt ggagatggtc ctgttttgct tccagacaat cattatttgt
      661 caactcagtc tgctttgtct aaagatccaa acgagaaaag agaccacatg atcttgttgg
      721 agtttgtcac cgccgctggt attacacacg gtatggacga gttgagtaag ctttaggttt
      781 tagccttaga catgactgtt cctcagttca agttgggcac ttacgagaag accggtcttg
      841 ctagattcta atcaagagga tgtcagaatg ccatttgcct gagagatgca ggcttcattt
      901 ttgatacttt tttatttgta acctatatag tataggattt tttttgtcat tttgtttctt
      961 ctcgtacgag cttgctcctg atcagcctat ctcgcagctg atgaatatct tgtggtaggg
     1021 gtttgggaaa atcattcgag tttgatgttt ttcttggtat ttcccactcc tcttcagagt
     1081 acagaagatt aagtgagacc ttcgtttgtg cggatccgcc ggccctggta cctcgcgaat
     1141 gcatctagag
//