BBa_K945005 1 BBa_K945005 Pichia pastoris optimized His-GFP-PTS1 2012-09-26T11:00:00Z 2015-05-08T01:13:48Z This sequence is a modification and optimization of the original wild-type GFP found in Aequorea victoria. This sequence codes for a green fluorescent protein (GFP) which has been codon optimized for Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (TPS1) in its C-terminus. false false _1212_ 0 13149 9 It's complicated false During codon optimization, restriction sites inside the coding sequence had to be avoided for it to be compatible with assembly standards. false Daniel Villalobos Ram??rez annotation2204786 1 6xHis-tag range2204786 1 42 59 annotation2204787 1 PTS1 range2204787 1 765 773 annotation2204789 1 Yeast RBS range2204789 1 26 38 annotation2203339 1 wt-GFP range2203339 1 39 776 annotation2204785 1 AOX1 terminator range2204785 1 777 1117 BBa_K945005_sequence 1 attcgcgacccgggcactcggccggccccgccgccaccatgcaccatcatcatcatcattctaaaggagaggagttgttcacaggagtcgttccaattcttgttgagcttgacggagacgttaatggacaaaaattttctgtttccggtgaaggagagggagatgccacatacggaaagttgacccttaaattcatttgtactactggtaaattgcctgttccttggccaacacttgtcaccactttttcctacggtgtccaatgcttctcaagatatcctgatcatatgaagcagcacgactttttcaaatccgctatgccagaaggatatgttcaggagagaactattttctacaaggatgacggtaactacaagactagagccgaggttaagttcgagggagatacattggtcaacagaatcgaattgaagggtatcgatttcaaggaggacggaaatatcttgggtcataagatggaatacaactacaacagtcacaacgtttacattatggcagacaagcctaaaaacggaatcaaggtcaacttcaagatcagacataatatcaaggatggttcagttcaattggctgaccactaccaacagaacacccctattggagatggtcctgttttgcttccagacaatcattatttgtcaactcagtctgctttgtctaaagatccaaacgagaaaagagaccacatgatcttgttggagtttgtcaccgccgctggtattacacacggtatggacgagttgagtaagctttaggttttagccttagacatgactgttcctcagttcaagttgggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatttgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtataggattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgcagctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttcttggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggatccgccggccctggtacctcgcgaatgcatctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z