LOCUS       BBa_K945006             1352 bp    DNA     linear       14-May-2024
DEFINITION  Pichia pastoris optimized His-CrtE-PTS1
ACCESSION   BBa_K945006
VERSION     BBa_K945006.1  
FEATURES             Location/Qualifiers
     CDS             20..982
                     /label=CrtE
     RBS             8..19
                     /label=Yeast RBS
     misc_feature    23..40
                     /label=6xHis-tag
     misc_feature    971..979
                     /label=PTS1
     misc_feature    983..1323
                     /label=AOX1 terminator
ORIGIN
        1 ccgatcgccc gccgccacca tgcaccacca ccaccaccac tacccattta ttagaaccgc
       61 tagaatgacc gtctgcgcca aaaagcacgt ccaccttact agagacgctg ccgaacaatt
      121 gcttgctgat attgacagaa gattggatca gttgcttcca gttgaaggtg aaagagatgt
      181 tgtcggagca gctatgagag agggtgcctt ggcaccagga aagagaatca gacctatgtt
      241 gcttttgctt actgcaagag atttgggttg tgctgtttca catgatggat tgcttgactt
      301 ggcttgcgcc gttgaaatgg tccacgccgc aagtttgatt cttgatgaca tgccatgtat
      361 ggatgacgct aaattgagaa gaggtagacc tactattcat tctcactacg gagagcatgt
      421 tgctatcttg gctgccgtcg ccttgctttc caaggcattt ggtgttattg cagatgctga
      481 cggattgaca ccacttgcaa aaaacagagc tgtttcagaa ttgagtaatg ctatcggtat
      541 gcaaggattg gtccaaggtc aattcaagga tctttctgaa ggagacaaac ctagatccgc
      601 cgaggcaatt ttgatgacca accattttaa gacttccaca cttttctgtg cttcaatgca
      661 gatggccagt atcgttgcta atgcctcttc cgaggctaga gattgcttgc acagattttc
      721 tttggatttg ggtcaagctt tccagttgct tgatgacttg acagatggta tgaccgacac
      781 tggaaaggat tcaaatcaag atgctggtaa gtctactttg gttaatttgc ttggacctag
      841 agccgtcgaa gagagattga gacaacattt gcaccttgca tctgaacact tgtccgcagc
      901 ttgccagcat ggtcacgcta cacagcattt tattcaagcc tggttcgata agaagttggc
      961 cgccgttagt tccaagttgt aggttttagc cttagacatg actgttcctc agttcaagtt
     1021 gggcacttac gagaagaccg gtcttgctag attctaatca agaggatgtc agaatgccat
     1081 ttgcctgaga gatgcaggct tcatttttga tactttttta tttgtaacct atatagtata
     1141 ggattttttt tgtcattttg tttcttctcg tacgagcttg ctcctgatca gcctatctcg
     1201 cagctgatga atatcttgtg gtaggggttt gggaaaatca ttcgagtttg atgtttttct
     1261 tggtatttcc cactcctctt cagagtacag aagattaagt gagaccttcg tttgtgcgga
     1321 tcccgatacg tactgtcgcg accaatctag ag
//