BBa_K945006 1 BBa_K945006 Pichia pastoris optimized His-CrtE-PTS1 2012-09-26T11:00:00Z 2015-05-08T01:13:48Z This sequence is originally derived from the CDS for geranylgeranyl pyrophosphate synthase from Pantoea ananatis. This sequence codes for CrtE (GGPP synthase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. false false _1212_ 0 13149 9 It's complicated false The codon optimization for the sequence had to take into account the illegal restriction sites to be avoided in order to be compatible with assembly standards. false Daniel Villalobos Ram??rez annotation2203340 1 CrtE range2203340 1 20 982 annotation2204796 1 PTS1 range2204796 1 971 979 annotation2204797 1 AOX1 terminator range2204797 1 983 1323 annotation2204795 1 6xHis-tag range2204795 1 23 40 annotation2204794 1 Yeast RBS range2204794 1 8 19 BBa_K945006_sequence 1 ccgatcgcccgccgccaccatgcaccaccaccaccaccactacccatttattagaaccgctagaatgaccgtctgcgccaaaaagcacgtccaccttactagagacgctgccgaacaattgcttgctgatattgacagaagattggatcagttgcttccagttgaaggtgaaagagatgttgtcggagcagctatgagagagggtgccttggcaccaggaaagagaatcagacctatgttgcttttgcttactgcaagagatttgggttgtgctgtttcacatgatggattgcttgacttggcttgcgccgttgaaatggtccacgccgcaagtttgattcttgatgacatgccatgtatggatgacgctaaattgagaagaggtagacctactattcattctcactacggagagcatgttgctatcttggctgccgtcgccttgctttccaaggcatttggtgttattgcagatgctgacggattgacaccacttgcaaaaaacagagctgtttcagaattgagtaatgctatcggtatgcaaggattggtccaaggtcaattcaaggatctttctgaaggagacaaacctagatccgccgaggcaattttgatgaccaaccattttaagacttccacacttttctgtgcttcaatgcagatggccagtatcgttgctaatgcctcttccgaggctagagattgcttgcacagattttctttggatttgggtcaagctttccagttgcttgatgacttgacagatggtatgaccgacactggaaaggattcaaatcaagatgctggtaagtctactttggttaatttgcttggacctagagccgtcgaagagagattgagacaacatttgcaccttgcatctgaacacttgtccgcagcttgccagcatggtcacgctacacagcattttattcaagcctggttcgataagaagttggccgccgttagttccaagttgtaggttttagccttagacatgactgttcctcagttcaagttgggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatttgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtataggattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgcagctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttcttggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggatcccgatacgtactgtcgcgaccaatctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z