LOCUS       BBa_K945007             1564 bp    DNA     linear       23-Dec-2024
DEFINITION  Pichia pastoris optimized His-CrtB-PTS1
ACCESSION   BBa_K945007
VERSION     BBa_K945007.1  
FEATURES             Location/Qualifiers
     CDS             41..997
                     /label=CrtB
     RBS             29..40
                     /label=Yeast RBS
     misc_feature    998..1338
                     /label=AOX1 Terminator
     misc_feature    44..61
                     /label=6xHis-tag
     misc_feature    986..994
                     /label=PTS1
ORIGIN
        1 atccaattgc cgagctcttc cccgctcgcc cgccgccacc atgcaccacc atcaccacca
       61 caataatcct tccttgctta accacgccgt cgagacaatg gccgtcggtt caaagtcctt
      121 cgctacagca tcaaagttgt ttgacgctaa gactagaaga agtgttctta tgttgtacgc
      181 ttggtgtaga cattgcgatg acgtcattga tgaccaaacc ttgggtttcc aagctagaca
      241 gccagccttg caaactcctg aacagagact tatgcaattg gagatgaaga caagacaggc
      301 ttatgccgga tcacaaatgc acgaaccagc ttttgctgcc ttccaagagg ttgcaatggc
      361 tcatgatatt gcccctgcat acgcttttga ccacttggaa ggtttcgcta tggatgtcag
      421 agaggcacaa tactctcagc ttgatgacac tttgagatac tgttatcatg ttgctggtgt
      481 tgtcggattg atgatggctc agatcatggg tgtcagagac aacgccacct tggatagagc
      541 atgtgacctt ggattggcct ttcaattgac taacattgct agagatatcg ttgatgacgc
      601 ccatgcaggt agatgctact tgcctgcttc ttggttggaa cacgagggac ttaacaaaga
      661 aaattatgca gctcctgaga acagacaggc tttgagtaga atcgccagaa gacttgttca
      721 agaagctgag ccatactatt tgtctgctac agccggtctt gctggacttc ctttgagatc
      781 cgcatgggct attgccactg caaagcaggt ttacagaaag atcggtgtta aagtcgaaca
      841 agcaggacaa caggcttggg atcaaagaca gtctactaca accccagaaa aattgacttt
      901 gcttttggcc gcatctggac aagctttgac atccagaatg agagcccacc cacctagacc
      961 agcacacctt tggcagagac cacttagtaa attgtaggtt ttagccttag acatgactgt
     1021 tcctcagttc aagttgggca cttacgagaa gaccggtctt gctagattct aatcaagagg
     1081 atgtcagaat gccatttgcc tgagagatgc aggcttcatt tttgatactt ttttatttgt
     1141 aacctatata gtataggatt ttttttgtca ttttgtttct tctcgtacga gcttgctcct
     1201 gatcagccta tctcgcagct gatgaatatc ttgtggtagg ggtttgggaa aatcattcga
     1261 gtttgatgtt tttcttggta tttcccactc ctcttcagag tacagaagat taagtgagac
     1321 cttcgtttgt gcggatcccg actgcaggag tcgcgaccca atcggatccc gccattcgcc
     1381 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     1441 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     1501 gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacctcgc gaatgcatct
     1561 agag
//