BBa_K945007 1 BBa_K945007 Pichia pastoris optimized His-CrtB-PTS1 2012-09-26T11:00:00Z 2015-05-08T01:13:48Z This sequence is originally derived from the CDS for phytoene synthase from Pantoea ananatis. This sequence codes for CrtB (Phytoene synthase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. false false _1212_ 0 13149 9 It's complicated false The codon optimization for the sequence had to take into account the illegal restriction sites to be avoided in order to be compatible with assembly standards. false Daniel Villalobos Ram??rez annotation2204792 1 6xHis-tag range2204792 1 44 61 annotation2204793 1 PTS1 range2204793 1 986 994 annotation2204791 1 AOX1 Terminator range2204791 1 998 1338 annotation2203341 1 CrtB range2203341 1 41 997 annotation2204790 1 Yeast RBS range2204790 1 29 40 BBa_K945007_sequence 1 atccaattgccgagctcttccccgctcgcccgccgccaccatgcaccaccatcaccaccacaataatccttccttgcttaaccacgccgtcgagacaatggccgtcggttcaaagtccttcgctacagcatcaaagttgtttgacgctaagactagaagaagtgttcttatgttgtacgcttggtgtagacattgcgatgacgtcattgatgaccaaaccttgggtttccaagctagacagccagccttgcaaactcctgaacagagacttatgcaattggagatgaagacaagacaggcttatgccggatcacaaatgcacgaaccagcttttgctgccttccaagaggttgcaatggctcatgatattgcccctgcatacgcttttgaccacttggaaggtttcgctatggatgtcagagaggcacaatactctcagcttgatgacactttgagatactgttatcatgttgctggtgttgtcggattgatgatggctcagatcatgggtgtcagagacaacgccaccttggatagagcatgtgaccttggattggcctttcaattgactaacattgctagagatatcgttgatgacgcccatgcaggtagatgctacttgcctgcttcttggttggaacacgagggacttaacaaagaaaattatgcagctcctgagaacagacaggctttgagtagaatcgccagaagacttgttcaagaagctgagccatactatttgtctgctacagccggtcttgctggacttcctttgagatccgcatgggctattgccactgcaaagcaggtttacagaaagatcggtgttaaagtcgaacaagcaggacaacaggcttgggatcaaagacagtctactacaaccccagaaaaattgactttgcttttggccgcatctggacaagctttgacatccagaatgagagcccacccacctagaccagcacacctttggcagagaccacttagtaaattgtaggttttagccttagacatgactgttcctcagttcaagttgggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatttgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtataggattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgcagctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttcttggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggatcccgactgcaggagtcgcgacccaatcggatcccgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattcgagctcggtacctcgcgaatgcatctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z