BBa_K945007
1
BBa_K945007
Pichia pastoris optimized His-CrtB-PTS1
2012-09-26T11:00:00Z
2015-05-08T01:13:48Z
This sequence is originally derived from the CDS for phytoene synthase from Pantoea ananatis.
This sequence codes for CrtB (Phytoene synthase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus.
false
false
_1212_
0
13149
9
It's complicated
false
The codon optimization for the sequence had to take into account the illegal restriction sites to be avoided in order to be compatible with assembly standards.
false
Daniel Villalobos Ram??rez
annotation2204793
1
PTS1
range2204793
1
986
994
annotation2204791
1
AOX1 Terminator
range2204791
1
998
1338
annotation2204792
1
6xHis-tag
range2204792
1
44
61
annotation2204790
1
Yeast RBS
range2204790
1
29
40
annotation2203341
1
CrtB
range2203341
1
41
997
BBa_K945007_sequence
1
atccaattgccgagctcttccccgctcgcccgccgccaccatgcaccaccatcaccaccacaataatccttccttgcttaaccacgccgtcgagacaatggccgtcggttcaaagtccttcgctacagcatcaaagttgtttgacgctaagactagaagaagtgttcttatgttgtacgcttggtgtagacattgcgatgacgtcattgatgaccaaaccttgggtttccaagctagacagccagccttgcaaactcctgaacagagacttatgcaattggagatgaagacaagacaggcttatgccggatcacaaatgcacgaaccagcttttgctgccttccaagaggttgcaatggctcatgatattgcccctgcatacgcttttgaccacttggaaggtttcgctatggatgtcagagaggcacaatactctcagcttgatgacactttgagatactgttatcatgttgctggtgttgtcggattgatgatggctcagatcatgggtgtcagagacaacgccaccttggatagagcatgtgaccttggattggcctttcaattgactaacattgctagagatatcgttgatgacgcccatgcaggtagatgctacttgcctgcttcttggttggaacacgagggacttaacaaagaaaattatgcagctcctgagaacagacaggctttgagtagaatcgccagaagacttgttcaagaagctgagccatactatttgtctgctacagccggtcttgctggacttcctttgagatccgcatgggctattgccactgcaaagcaggtttacagaaagatcggtgttaaagtcgaacaagcaggacaacaggcttgggatcaaagacagtctactacaaccccagaaaaattgactttgcttttggccgcatctggacaagctttgacatccagaatgagagcccacccacctagaccagcacacctttggcagagaccacttagtaaattgtaggttttagccttagacatgactgttcctcagttcaagttgggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatttgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtataggattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgcagctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttcttggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggatcccgactgcaggagtcgcgacccaatcggatcccgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattcgagctcggtacctcgcgaatgcatctagag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z