LOCUS       BBa_K945008             1898 bp    DNA     linear       22-Nov-2024
DEFINITION  Pichia pastoris optimized His-CrtI-PTS1
ACCESSION   BBa_K945008
VERSION     BBa_K945008.1  
FEATURES             Location/Qualifiers
     CDS             20..1525
                     /label=CrtI
     RBS             8..19
                     /label=Yeast RBS
     misc_feature    23..40
                     /label=6xHis-tag
     misc_feature    1514..1522
                     /label=PTS1
     misc_feature    1526..1866
                     /label=AOX1 Terminator
ORIGIN
        1 taaatcgccc gccgccacca tgcaccacca ccaccaccat aaacctacca ccgtcatcgg
       61 agccggattc ggaggacttg cccttgccat tagacttcag gccgccggta ttccagtttt
      121 gcttttggaa caaagagata aaccaggtgg tagagcttac gtctatgagg atcagggttt
      181 tactttcgac gccggaccaa cagttattac cgacccttca gcaattgaag aattgtttgc
      241 tttggctggt aaacaattga aagaatacgt tgagcttttg ccagtcaccc ctttttatag
      301 attgtgttgg gaatccggaa aggttttcaa ctacgataat gaccaaacta gattggaagc
      361 ccagattcaa cagttcaacc caagagatgt tgagggttac agacaatttt tggactattc
      421 aagagctgtt ttcaaagagg gttacttgaa gttgggaact gtcccatttc ttagtttcag
      481 agatatgttg agagctgccc ctcaacttgc aaaattgcag gcttggagat cagtttacag
      541 taaggtcgca tcttatatcg aagacgagca cttgagacaa gctttttctt tccattccct
      601 tttggttggt ggaaatccat ttgctacttc ttccatctac acacttatcc acgccttgga
      661 aagagagtgg ggagtttggt ttcctagagg tggaactggt gctttggtcc aaggaatgat
      721 taaacttttc caggatttgg gtggagaagt tgtcttgaac gccagagttt cacacatgga
      781 gactacaggt aataagatcg aagcagtcca tcttgaggat ggaagaagat ttttgactca
      841 agctgttgcc agtaacgctg atgttgtcca cacatacaga gaccttttgt ctcagcatcc
      901 tgctgctgtt aagcaatcca acaaattgca gactaagaga atgtctaatt ccctttttgt
      961 tttgtacttc ggtcttaacc atcaccatga tcaattggct caccatacag tttgctttgg
     1021 tcctagatac agagaattga tcgacgaaat ttttaaccac gatggattgg ccgaagactt
     1081 ctctctttac ttgcacgctc catgtgttac tgattcaagt ttggctcctg agggttgcgg
     1141 ttcttactat gttttggcac cagtccctca tcttggtaca gctaacttgg attggaccgt
     1201 tgaaggacct aagcttagag acagaatttt tgaatacttg gagcaacact acatgccagg
     1261 tttgagatcc caattggtta cccatcagat gttcactcct tttgatttca gagaccaatt
     1321 gaatgcctac cagggttctg ctttttctgt tgaaccagtc ttgacacagt ccgcttggtt
     1381 cagacctcac aacagagata agaccatcac taatctttat ttggtcggtg ctggaaccca
     1441 tccaggtgcc ggaatccctg gtgttattgg ttccgctaaa gccactgctg gacttatgtt
     1501 ggaggacctt atttcaaagt tgtaggtttt agccttagac atgactgttc ctcagttcaa
     1561 gttgggcact tacgagaaga ccggtcttgc tagattctaa tcaagaggat gtcagaatgc
     1621 catttgcctg agagatgcag gcttcatttt tgatactttt ttatttgtaa cctatatagt
     1681 ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc
     1741 tcgcagctga tgaatatctt gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt
     1801 tcttggtatt tcccactcct cttcagagta cagaagatta agtgagacct tcgtttgtgc
     1861 ggatccaata ttcgagcggc cgcatcgcga atctagag
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