BBa_K945008 1 BBa_K945008 Pichia pastoris optimized His-CrtI-PTS1 2012-09-26T11:00:00Z 2015-05-08T01:13:48Z This sequence is originally derived from the CDS for phytoene desaturase from Pantoea ananatis. This sequence codes for CrtI (Phytoene desaturase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. false false _1212_ 0 13149 9 It's complicated false The codon optimization for the sequence had to take into account the illegal restriction sites to be avoided in order to be compatible with assembly standards. false Daniel Villalobos Ram??rez annotation2204799 1 6xHis-tag range2204799 1 23 40 annotation2204801 1 AOX1 Terminator range2204801 1 1526 1866 annotation2203342 1 CrtI range2203342 1 20 1525 annotation2204800 1 PTS1 range2204800 1 1514 1522 annotation2204798 1 Yeast RBS range2204798 1 8 19 BBa_K945008_sequence 1 taaatcgcccgccgccaccatgcaccaccaccaccaccataaacctaccaccgtcatcggagccggattcggaggacttgcccttgccattagacttcaggccgccggtattccagttttgcttttggaacaaagagataaaccaggtggtagagcttacgtctatgaggatcagggttttactttcgacgccggaccaacagttattaccgacccttcagcaattgaagaattgtttgctttggctggtaaacaattgaaagaatacgttgagcttttgccagtcacccctttttatagattgtgttgggaatccggaaaggttttcaactacgataatgaccaaactagattggaagcccagattcaacagttcaacccaagagatgttgagggttacagacaatttttggactattcaagagctgttttcaaagagggttacttgaagttgggaactgtcccatttcttagtttcagagatatgttgagagctgcccctcaacttgcaaaattgcaggcttggagatcagtttacagtaaggtcgcatcttatatcgaagacgagcacttgagacaagctttttctttccattcccttttggttggtggaaatccatttgctacttcttccatctacacacttatccacgccttggaaagagagtggggagtttggtttcctagaggtggaactggtgctttggtccaaggaatgattaaacttttccaggatttgggtggagaagttgtcttgaacgccagagtttcacacatggagactacaggtaataagatcgaagcagtccatcttgaggatggaagaagatttttgactcaagctgttgccagtaacgctgatgttgtccacacatacagagaccttttgtctcagcatcctgctgctgttaagcaatccaacaaattgcagactaagagaatgtctaattccctttttgttttgtacttcggtcttaaccatcaccatgatcaattggctcaccatacagtttgctttggtcctagatacagagaattgatcgacgaaatttttaaccacgatggattggccgaagacttctctctttacttgcacgctccatgtgttactgattcaagtttggctcctgagggttgcggttcttactatgttttggcaccagtccctcatcttggtacagctaacttggattggaccgttgaaggacctaagcttagagacagaatttttgaatacttggagcaacactacatgccaggtttgagatcccaattggttacccatcagatgttcactccttttgatttcagagaccaattgaatgcctaccagggttctgctttttctgttgaaccagtcttgacacagtccgcttggttcagacctcacaacagagataagaccatcactaatctttatttggtcggtgctggaacccatccaggtgccggaatccctggtgttattggttccgctaaagccactgctggacttatgttggaggaccttatttcaaagttgtaggttttagccttagacatgactgttcctcagttcaagttgggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatttgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtataggattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgcagctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttcttggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggatccaatattcgagcggccgcatcgcgaatctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z