BBa_K945008
1
BBa_K945008
Pichia pastoris optimized His-CrtI-PTS1
2012-09-26T11:00:00Z
2015-05-08T01:13:48Z
This sequence is originally derived from the CDS for phytoene desaturase from Pantoea ananatis.
This sequence codes for CrtI (Phytoene desaturase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus.
false
false
_1212_
0
13149
9
It's complicated
false
The codon optimization for the sequence had to take into account the illegal restriction sites to be avoided in order to be compatible with assembly standards.
false
Daniel Villalobos Ram??rez
annotation2204799
1
6xHis-tag
range2204799
1
23
40
annotation2204801
1
AOX1 Terminator
range2204801
1
1526
1866
annotation2203342
1
CrtI
range2203342
1
20
1525
annotation2204800
1
PTS1
range2204800
1
1514
1522
annotation2204798
1
Yeast RBS
range2204798
1
8
19
BBa_K945008_sequence
1
taaatcgcccgccgccaccatgcaccaccaccaccaccataaacctaccaccgtcatcggagccggattcggaggacttgcccttgccattagacttcaggccgccggtattccagttttgcttttggaacaaagagataaaccaggtggtagagcttacgtctatgaggatcagggttttactttcgacgccggaccaacagttattaccgacccttcagcaattgaagaattgtttgctttggctggtaaacaattgaaagaatacgttgagcttttgccagtcacccctttttatagattgtgttgggaatccggaaaggttttcaactacgataatgaccaaactagattggaagcccagattcaacagttcaacccaagagatgttgagggttacagacaatttttggactattcaagagctgttttcaaagagggttacttgaagttgggaactgtcccatttcttagtttcagagatatgttgagagctgcccctcaacttgcaaaattgcaggcttggagatcagtttacagtaaggtcgcatcttatatcgaagacgagcacttgagacaagctttttctttccattcccttttggttggtggaaatccatttgctacttcttccatctacacacttatccacgccttggaaagagagtggggagtttggtttcctagaggtggaactggtgctttggtccaaggaatgattaaacttttccaggatttgggtggagaagttgtcttgaacgccagagtttcacacatggagactacaggtaataagatcgaagcagtccatcttgaggatggaagaagatttttgactcaagctgttgccagtaacgctgatgttgtccacacatacagagaccttttgtctcagcatcctgctgctgttaagcaatccaacaaattgcagactaagagaatgtctaattccctttttgttttgtacttcggtcttaaccatcaccatgatcaattggctcaccatacagtttgctttggtcctagatacagagaattgatcgacgaaatttttaaccacgatggattggccgaagacttctctctttacttgcacgctccatgtgttactgattcaagtttggctcctgagggttgcggttcttactatgttttggcaccagtccctcatcttggtacagctaacttggattggaccgttgaaggacctaagcttagagacagaatttttgaatacttggagcaacactacatgccaggtttgagatcccaattggttacccatcagatgttcactccttttgatttcagagaccaattgaatgcctaccagggttctgctttttctgttgaaccagtcttgacacagtccgcttggttcagacctcacaacagagataagaccatcactaatctttatttggtcggtgctggaacccatccaggtgccggaatccctggtgttattggttccgctaaagccactgctggacttatgttggaggaccttatttcaaagttgtaggttttagccttagacatgactgttcctcagttcaagttgggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatttgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtataggattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgcagctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttcttggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggatccaatattcgagcggccgcatcgcgaatctagag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z