LOCUS BBa_K948200 8093 bp DNA linear 16-Dec-2024 DEFINITION big P ACCESSION BBa_K948200 VERSION BBa_K948200.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 misc_feature 157..204 /label=nanotag misc_feature 205..213 /label=GSA linker misc_feature 214..231 /label=His tag misc_feature 232..240 /label=GSA linker misc_feature 241..260 /label=TEV protease site misc_feature 261..270 /label=GSA linker misc_feature 271..276 /label=NheI misc_feature 277..285 /label=GSA linker misc_feature 286..306 /label=TEV protease site misc_feature 307..315 /label=GSA linker misc_feature 316..333 /label=His tag misc_feature 334..342 /label=GSA linker misc_feature 343..891 /label=Secretion tag misc_feature 892..1021 /label=terminator (B0015) misc_feature 157..1021 /label=BBa_K215001 RBS 1118..1129 /label=rbs stem_loop 2418..2545 /label=stem_loop promoter 1030..1109 /label=promoter misc_feature 2389..2406 /label=tag CDS 1130..2382 /label=protein misc_feature 1030..2545 /label=BBa_K875004 promoter 2554..2684 /label=promoter misc_feature 2593..2610 /label=AraI1 misc_feature 2614..2631 /label=AraI2 promoter 2554..2683 /label=BBa_K206000 RBS 2694..2709 /label=native RBS CDS 2710..4440 /label=prtD CDS 2692..4440 /label=BBa_K215100 RBS 4451..4463 /label=native rbs CDS 4464..5810 /label=prtE CDS 4449..5810 /label=BBa_K215101 RBS 5821..5832 /label=Native RBS CDS 5833..7221 /label=prtF CDS 5819..7221 /label=BBa_K215102 misc_feature 2692..7221 /label=BBa_K215104 stem_loop 7241..7284 /label=stem_loop misc_feature 7230..7309 /label=BBa_B0010 terminator 7230..7309 /label=BBa_B0010 stem_loop 7325..7344 /label=T7 TE misc_feature 7351..7351 /label=stop polyA_site 7345..7358 /label=polya misc_feature 7318..7358 /label=BBa_B0012 terminator 7318..7358 /label=BBa_B0012 terminator 7230..7358 /label=BBa_B0015 promoter 7367..7451 /label=BBa_K875001 misc_feature 7466..7469 /label=conserved RBS 7460..7473 /label=BBa_B0031 misc_feature 7480..7956 /label=tse2 CDS 7480..7956 /label=BBa_K314200 stem_loop 7976..8019 /label=stem_loop misc_feature 7965..8044 /label=BBa_B0010 terminator 7965..8044 /label=BBa_B0010 stem_loop 8060..8079 /label=T7 TE misc_feature 8086..8086 /label=stop polyA_site 8080..8093 /label=polya misc_feature 8053..8093 /label=BBa_B0012 terminator 8053..8093 /label=BBa_B0012 terminator 7965..8093 /label=BBa_B0015 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatgg acgttgaagc atggctgggt 181 gcccgtgtac cgctggttga aacgggcagc gcacatcacc accatcatca tggttccgcc 241 gaaaacctct actttcaggg cggctctgct gctagcggtt ctgccgagaa tctgtacttc 301 caaggtggct ccgctcatca ccaccatcat cacggtagcg caggcggcac tgacaccttt 361 gacttctctg gttactctaa caatcagcgt atcaacctga acgagggttc tttctctgac 421 gttggtggtc tgaagggcaa cgtttctatc gcgcatggtg ttaccatcga aaacgcgatc 481 ggtggttctg gtaacgacat cctgatcggt aatggcgcgg ataacatcct ccagggtggt 541 gcgggtgacg acgttctgta cggttctacg ggtgcggaca cgctgacggg tggcgctggt 601 cgtgacattt tcgtatatgg tagcggtcaa gactctaccg tttctgcata cgactggatc 661 acggattttc agacgggtat cgacaaaatc gacctgtccg ctttccgtaa tgaaggtcag 721 ctgtcttttg ttcaggacca gtttactggt aaaggccagg aggttatgct gcaatgggac 781 gcggcgaact ctacgaccaa tctgtggctg cacgaagcgg gtcactcttc tgttgacttt 841 ctcgttcgta tcgttggcca gaccgcgcag agcgacatta tcgtctaata accaggcatc 901 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 961 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1021 ttactagaga atcataaaaa atttatttgc tttgtgagcg gataacaatt ataatagatt 1081 caattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat actagaaaga 1141 ggagaaatac taatgaaagc tactaaactg gtactgggcg cggtaatcct gggttctact 1201 ctgctggcag gttgctccag caacgctaaa atcgatcagg gaattaaccc gtatgttggc 1261 tttgaaatgg gttacgactg gttaggtcgt atgccgtaca aaggcagcgt tgaaaacggt 1321 gcatacaaag ctcagggcgt tcaactgacc gctaaactgg gttacccaat cactgacgac 1381 ctggacatct acactcgtct gggtggcatg gtatggcgtg cagacactaa atccaacgtt 1441 tatggtaaaa accacgacac cggcgtttct ccggtcttcg ctggcggtgt tgagtacgcg 1501 atcactcctg aaatcgctac ccgtctggaa taccagtgga ccaacaacat cggtgacgca 1561 cacaccatcg gcactcgtcc ggacaacggc ggaggttctg gagatattct gctgacccag 1621 agcccggcga ttctgagcgt gagcccgggc gaacgcgtga gctttagctg ccgcgcgagc 1681 cagagcattg gcgtgctgtg cgatcgcatt agcattcatt ggtatcagca gcgcaccaac 1741 ggcagcccgc gcctgctgat taaatatgcg agcgaaagca ttagcggcat tccgagccgc 1801 tttagcggca gcggcagcgg caccgatttt accctgagca ttaacagcgt ggaaagcgaa 1861 gatattgcgg attattattg ccagcagagc aacagctggc cgtggacctt tggcggcggc 1921 accaaactgg aaattaaagg cggcaaaggc agcggcggca aaggcaccgg cggcaaaggc 1981 agcggcggca aaggcagcga agtgaaactg gtggaaagcg gcggcgatct ggtgcagccg 2041 ggcggcagcc tgaaactgag ctgcgcggtg cattgcgatc gcaccagcgg ctttaccttt 2101 agcgattatt atatgtattg ggtgcgccag accccggaaa aacgcctgga atgggtggcg 2161 tatattagca gcgtgcattg cgatcgcggc ggcggcagca ccttttatcc agataccgtg 2221 aaaggccgct ttaccattag ccgcgataac gcgaaaaaca ccctgtatct acagatgagc 2281 cgcctgaaaa gcgatgatac cgcgatgtat tattgcgcgc gcgcgtatca tggcaactat 2341 tttgattatt ggggccaggg caccaccctg accgtgagca gctccggtca ccaccaccac 2401 caccactgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 2461 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctgctcacct 2521 tcgggtgggc ctttctgcgt ttatatacta gagacattga ttatttgcac ggcgtcacac 2581 tttgctatgc catagcaaga tagtccataa gattagcgga tcctacctga cgctttttat 2641 cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga gaggggagag 2701 agccctgcca tgaacgcgtc tagcgaacgt gaccgttctc tgtttggtgt gctgcgtcag 2761 ttccgccgct ccttttggtc tgttggcatc ttcagcgcgg tcatcaatgt tctgatgctg 2821 gcacctagcg tctacatgct ccaggtttac gaccgtgtcc tcgccagcgg caacggtatt 2881 actctgctga tgctcacgct cctgatggcg ggtctgtgcg ccttcatggg tgctctcgaa 2941 tgggttcgca gcctgctggt agttcgtctc ggtactcgca tcgacctcgc gctcaatcaa 3001 gacgttttta acgcggcttt cgcgcgtaat ctggaagctg gtgatggccg tgccggtctg 3061 gcgctgaccg acctcaccct gctccgtcaa ttcatcacgg gtaatgcact gttcgcgttc 3121 ttcgacgttc cgtggttccc gctctttctc ctcgtactgt tcctgctgca cccttggctc 3181 ggcatgctcg cactgggtgg tacggttgta cctggtggcg taggcctcgc agaaccggcg 3241 tctgaccagt ctaccgcggg tggctctaat caacaaagcc aacaagccac tcatctcgcg 3301 gatgcgcagc tccgtaatgc ggacgttatc gaagcgatgg gtatgctcgg taacctccgt 3361 cgtcgctggc tggcgcgtca ctaccgcttt atctccctcc aaaatctcgc ttccgagcgc 3421 gcagccgccg ttggtggtgc gtccaaatac tctcgtattg cactccaaag cctcatgctg 3481 ggcctcggtg cgctcctcgc gatcgacggc aaaatcacgc cgggtatgat gattgcgggc 3541 tctatcctcg tcggtcgtgt tctgtccccg attgaccagc tgatcggtgt ttggaaacag 3601 tggtcttctg cccgcattgc gtggcagcgc ctgacccgtc tgattgccgc atatccgcca 3661 cgccctgcgg ctatggctct cccagcgccg gaaggccacc tgtccgttga gcaagtttct 3721 ctgcgtaccg cacaaggcaa tactcgcctg caaaacatcc atttctccct gcaagcgggt 3781 gagaccctgg ttatcctggg cgcctctggt tctggcaaat cttctctcgc tcgtctgctc 3841 gtgggtgcac aatcccctac tcaaggtaaa gttcgcctcg atggtgcgga cctgaaccaa 3901 gttgacaaaa acaccttcgg tccgaccatt ggttacctcc cgcaggacgt tcaactgttc 3961 aaaggtagcc tggcggaaaa cattgcccgt ttcggcgacg ccgacccgga aaaggttgtt 4021 gcggcagcta agctcgccgg tgttcacgaa ctcatcctgt ctctgcctaa tggttacgac 4081 actgagctgg gtgacggcgg tggcggcctg tctggtggtc aacgtcagcg tatcggtctc 4141 gcacgcgcaa tgtacggcga cccgtgtctc ctcattctgg acgaaccgaa cgcatctctg 4201 gactctgagg gtgaccaagc actcatgcag gctattgtgg cgctccagaa acgtggtgca 4261 accgttgtac tcattacgca ccgtccggca ctcaccaccc tcgctcagaa aatcctgatc 4321 ctccacgagg gtcaacagca acgtatgggc ctggcccgtg acgttctgac ggaactccag 4381 cagcgctccg cggcaaacca ggcgcgtatg aacccgaccg cagcaatgcc acagtaataa 4441 tactagagag cagggatctc gtgatgactg gtatggatat taccactcag gacgaactca 4501 acgaagctgc catgcgtgac cgtgcgagcc gtgacgaaga acgtgcgctc cgtctgggtt 4561 ggtggctcgt cctcgcgggt ttcggtggtt tcctcctgtg ggcgctcctg gctccactgg 4621 acaagggtgt tgcggttcag ggtaacgttg tagtttccgg taaccgtaaa gttatccagc 4681 acatgcaagg cggtattgtt gaccgcatcc aagttaagga cggcgaccgt gttgccgctg 4741 gtcaggttct gctcactctc aatgcagtcg acgctcgtac cacctccgag ggcctgggtt 4801 ctcagtacga tcagctcatc gcgcgtgaag cgcgcctcct cgccgagcag cgtaaccagt 4861 cttctctcgc tgcaacgcca cgcctgacgc aggcacgcca gcgtccggaa atggcggcga 4921 ttatcgccct ccaggaagac ctgctgcgct ctcgtcagca gtctctcaaa ctggaaattg 4981 acggtgtccg tgcatctatc gatggtctgg aaacttctct gggtgctctg caaaaagtga 5041 tgtcttctaa acagtctgaa caagcgactc tgtctcaaca gctgcaaggt ctgcgtccgc 5101 tggccgcaga caactacgtt ccgcgtaaca agatgctcga aaccgaacgt ctcttcgccc 5161 aggtttctgg cgagctggcc cagacctctg gtgaagttgg tcgtacccgt cgtgacattc 5221 aacaacagaa actccgcatt gcgcagcgcc agcaggagta cgacaaagaa gtcaactctg 5281 aactgtctga cgttcaggcc aaactgaacg aagtaatctc tcagcgtgaa aaggcggact 5341 tcaatctcgc taacgtgcag gttcgtgcgc cggtggcggg cactgtggtg gatatgaaaa 5401 tcttcaccga aggtggcgta attgctccgg gtcaagttat gatggacatt gttccggagg 5461 atcagccact gctggtagat ggccgtatcc cggttgagat ggttgacaaa gtatggagcg 5521 gtctgccggt cgaactgcaa tttactgcgt tttcccaatc tactacgccg cgtgtccctg 5581 gtactgtaac cctgctgagc gcggatcgcc tggtggacga aaaagatggc accccttact 5641 atggcctccg tattcaggtg tctgaggaag gtaaacgtag cctccacggc ctggagatta 5701 aaccgggtat gcctgtccaa ggttttgtgc gtacgggcga acgctctttc atcaactacc 5761 tcttcaaacc gctcatggac cgtatgcacc tcgcactgac cgaagaataa tactagagag 5821 cggaaggaag caatgcgccg taaagcagtt ctgctcactg tggttctgag cctgtctggt 5881 ggtagcgccc aagcgatggg tctgctggac gcgtgggaac tcgcgctccg caatgatgcg 5941 caactgcgtg cggcaggttt cgaacgtgac gcgggtcaag aggaagttgc gattggtcgc 6001 gcaggcctcc tgccgagcct gcaatacacg tacggtgcga actactccca ctccaaagtt 6061 acccagcgcg atcgtaccct gaacaacacc acgaaacgtg attatgataa ctacgtttct 6121 acgctgaccc tgcgtcagcc actcctggac tacgcggcat gggcgcgtta tcagcaaggc 6181 gtaactcgta aactgatggc ggaccagcgt tttcgcgacc gttcccagga tctgatggtt 6241 cgtctgtacc agtcctggag cgaagccctg ctggcgcagg aaaagctgat gctcctcgac 6301 gctcagcgtc gtgcatacca ggaacagctc gcactgaatc gccgtctcct ggctgccggt 6361 gagggcacgc aaaccgacct gcgtgagacc gaagcacgtt ataccgtcac cgaggcgcag 6421 cgtatcgaac aggaggacac cctcgatgcc gccatgactg acctggaaaa catgatgggc 6481 tctcctctcc agatccaaga cctgtctcca ctggctctcg acactctccc ggataacgta 6541 acggaaaacc gtagcctctc tcagtggcgt gaactcacgg tacgtcacaa cgccaaactg 6601 gcagtacaac gtgagaacgt ggattactct cgctacgaaa tcgagcgcaa ccgtgcgggc 6661 cacctcccga ccctggacct ggttgcctct acccgtaatt ccctctccga gagcgaatac 6721 aactacaatc aaaaatacga cacccagacc gtaggtctgc aagttcgtgt tcctctctac 6781 tctggcggtg cggtttctgc gtctatgcgc caagcggcag ctgagtatca acagtcccag 6841 gcggaactgg acaatcagac tcgtcagacc ttcgcggagc tgcgtcgcca gttcaacctg 6901 tgcgcaaacg gtgctgcgaa aatccgtgct tggcagatgt ctgtagctgc tgccgaagag 6961 gccattcgtg cgacccgtca gtctgttgcg ggtggtgaac gtattaacct ggatgtgctc 7021 atggctgagc aggaatggta taatgcccgt cgtgagctga cggaggttaa gtaccgttgg 7081 ctgcaagcgt ggctcaatct gcgctacacg gctggtactc tcaacgaaca agatatgatg 7141 cagctggcag cctggttcca gtctgcgccg gttatcaata aaaccggcat caatgctgcc 7201 acgggcaaca agaccaatta atactagagc caggcatcaa ataaaacgaa aggctcagtc 7261 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 7321 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaaat cataaaaaat 7381 ttatttgctt tgtgagcgga taacaattat aatagattca acaaacagac aatctggtct 7441 gtttgtatta ttactagagt cacacaggaa acctactaga tgtcctacga ctacgagaaa 7501 accagcctca ccctctaccg ggcggtattc aaggccaact acgacggcga cgtcggtcgc 7561 tacctgcatc ccgacaagga actcgccgag gctgcggaag tcgccccgct gctgcatccg 7621 accttcgaca gccccaacac ccctggcgtc cccgcccgcg cgccggacat cgtcgccggc 7681 cgcgacggcc tctacgcccc ggacaccggc ggcacctcgg tgttcgaccg cgccggcgtg 7741 ctgcgccgcg ccgacggcga cttcgtgata cccgacggca ccgacatccc gccggacctt 7801 aaggtgaagc aggacagcta caacaagcgc ctgcaagcca cccactacac catcatgccg 7861 gccaagccga tgtaccggga ggtcctcatg ggccaactgg acaacttcgt gcgcaacgcc 7921 atccgccgcc aatgggaaaa agcccgcggg ctctagtact agagccaggc atcaaataaa 7981 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 8041 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //