##gff-version 3
##sequence-region BBa_K948200 1 8093
BBa_K948200	.	terminator	7230	7358	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K948200	.	terminator	7230	7309	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K948200	.	engineered_region	7230	7309	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K948200	.	stem_loop	7241	7284	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K948200	.	terminator	7318	7358	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K948200	.	engineered_region	7318	7358	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K948200	.	stop_codon	7351	7351	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K948200	.	stem_loop	7325	7344	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K948200	.	polyA_site	7345	7358	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K948200	.	CDS	7480	7956	.	+	0	ID=BBa_K314200;Name=BBa_K314200
BBa_K948200	.	engineered_region	7480	7956	.	+	0	ID=annotation2089456;Name=tse2;Parent=BBa_K314200
BBa_K948200	.	engineered_region	2692	7221	.	+	0	ID=BBa_K215104;Name=BBa_K215104
BBa_K948200	.	CDS	5819	7221	.	+	0	ID=BBa_K215102;Name=BBa_K215102;Parent=BBa_K215104
BBa_K948200	.	CDS	5833	7221	.	+	0	ID=annotation2027450;Name=prtF;Parent=BBa_K215102
BBa_K948200	.	ribosome_entry_site	5821	5832	.	+	0	ID=annotation2027449;Name=Native RBS;Parent=BBa_K215102
BBa_K948200	.	CDS	4449	5810	.	+	0	ID=BBa_K215101;Name=BBa_K215101;Parent=BBa_K215104
BBa_K948200	.	CDS	4464	5810	.	+	0	ID=annotation2027448;Name=prtE;Parent=BBa_K215101
BBa_K948200	.	ribosome_entry_site	4451	4463	.	+	0	ID=annotation2027447;Name=native rbs;Parent=BBa_K215101
BBa_K948200	.	CDS	2692	4440	.	+	0	ID=BBa_K215100;Name=BBa_K215100;Parent=BBa_K215104
BBa_K948200	.	CDS	2710	4440	.	+	0	ID=annotation2027446;Name=prtD;Parent=BBa_K215100
BBa_K948200	.	ribosome_entry_site	2694	2709	.	+	0	ID=annotation2027445;Name=native RBS;Parent=BBa_K215100
BBa_K948200	.	promoter	7367	7451	.	+	0	ID=BBa_K875001;Name=BBa_K875001
BBa_K948200	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K948200	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K948200	.	tag	157	1021	.	+	0	ID=BBa_K215001;Name=BBa_K215001
BBa_K948200	.	sequence_feature	241	260	.	+	0	ID=annotation2027591;Name=TEV protease site;Parent=BBa_K215001
BBa_K948200	.	sequence_feature	232	240	.	+	0	ID=annotation2027590;Name=GSA linker;Parent=BBa_K215001
BBa_K948200	.	sequence_feature	334	342	.	+	0	ID=annotation2027598;Name=GSA linker;Parent=BBa_K215001
BBa_K948200	.	stop_codon	892	1021	.	+	0	ID=annotation2027600;Name=terminator (B0015);Parent=BBa_K215001
BBa_K948200	.	tag	316	333	.	+	0	ID=annotation2027597;Name=His tag;Parent=BBa_K215001
BBa_K948200	.	tag	343	891	.	+	0	ID=annotation2027599;Name=Secretion tag;Parent=BBa_K215001
BBa_K948200	.	sequence_feature	205	213	.	+	0	ID=annotation2027588;Name=GSA linker;Parent=BBa_K215001
BBa_K948200	.	tag	214	231	.	+	0	ID=annotation2027589;Name=His tag;Parent=BBa_K215001
BBa_K948200	.	tag	157	204	.	+	0	ID=annotation2027587;Name=nanotag;Parent=BBa_K215001
BBa_K948200	.	sequence_feature	307	315	.	+	0	ID=annotation2027596;Name=GSA linker;Parent=BBa_K215001
BBa_K948200	.	sequence_feature	271	276	.	+	0	ID=annotation2027593;Name=NheI;Parent=BBa_K215001
BBa_K948200	.	sequence_feature	286	306	.	+	0	ID=annotation2027595;Name=TEV protease site;Parent=BBa_K215001
BBa_K948200	.	sequence_feature	277	285	.	+	0	ID=annotation2027594;Name=GSA linker;Parent=BBa_K215001
BBa_K948200	.	sequence_feature	261	270	.	+	0	ID=annotation2027592;Name=GSA linker;Parent=BBa_K215001
BBa_K948200	.	terminator	7965	8093	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K948200	.	terminator	7965	8044	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K948200	.	engineered_region	7965	8044	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K948200	.	stem_loop	7976	8019	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K948200	.	terminator	8053	8093	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K948200	.	engineered_region	8053	8093	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K948200	.	stop_codon	8086	8086	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K948200	.	stem_loop	8060	8079	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K948200	.	polyA_site	8080	8093	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K948200	.	promoter	1	130	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K948200	.	operator	61	78	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K948200	.	promoter	1	131	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K948200	.	operator	40	57	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K948200	.	ribosome_entry_site	7460	7473	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K948200	.	conserved_region	7466	7469	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K948200	.	promoter	2554	2683	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K948200	.	operator	2614	2631	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K948200	.	promoter	2554	2684	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K948200	.	operator	2593	2610	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K948200	.	engineered_region	1030	2545	.	+	0	ID=BBa_K875004;Name=BBa_K875004
BBa_K948200	.	promoter	1030	1109	.	+	0	ID=annotation2194271;Name=promoter;Parent=BBa_K875004
BBa_K948200	.	tag	2389	2406	.	+	0	ID=annotation2194273;Name=tag;Parent=BBa_K875004
BBa_K948200	.	ribosome_entry_site	1118	1129	.	+	0	ID=annotation2194268;Name=rbs;Parent=BBa_K875004
BBa_K948200	.	CDS	1130	2382	.	+	0	ID=annotation2194279;Name=protein;Parent=BBa_K875004
BBa_K948200	.	stem_loop	2418	2545	.	+	0	ID=annotation2194270;Name=stem_loop;Parent=BBa_K875004
>BBa_K948200
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggacgttgaagcatggctgggtgcccgtgtaccgctggttgaaacgggcagcgcacatcaccaccatcatcatggttcc
gccgaaaacctctactttcagggcggctctgctgctagcggttctgccgagaatctgtacttccaaggtggctccgctc
atcaccaccatcatcacggtagcgcaggcggcactgacacctttgacttctctggttactctaacaatcagcgtatcaa
cctgaacgagggttctttctctgacgttggtggtctgaagggcaacgtttctatcgcgcatggtgttaccatcgaaaac
gcgatcggtggttctggtaacgacatcctgatcggtaatggcgcggataacatcctccagggtggtgcgggtgacgacg
ttctgtacggttctacgggtgcggacacgctgacgggtggcgctggtcgtgacattttcgtatatggtagcggtcaaga
ctctaccgtttctgcatacgactggatcacggattttcagacgggtatcgacaaaatcgacctgtccgctttccgtaat
gaaggtcagctgtcttttgttcaggaccagtttactggtaaaggccaggaggttatgctgcaatgggacgcggcgaact
ctacgaccaatctgtggctgcacgaagcgggtcactcttctgttgactttctcgttcgtatcgttggccagaccgcgca
gagcgacattatcgtctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatattactag
agaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcac
acatactagagaaagaggagaaatactagaaagaggagaaatactaatgaaagctactaaactggtactgggcgcggta
atcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttg
aaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttca
actgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgca
gacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcga
tcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtcc
ggacaacggcggaggttctggagatattctgctgacccagagcccggcgattctgagcgtgagcccgggcgaacgcgtg
agctttagctgccgcgcgagccagagcattggcgtgctgtgcgatcgcattagcattcattggtatcagcagcgcacca
acggcagcccgcgcctgctgattaaatatgcgagcgaaagcattagcggcattccgagccgctttagcggcagcggcag
cggcaccgattttaccctgagcattaacagcgtggaaagcgaagatattgcggattattattgccagcagagcaacagc
tggccgtggacctttggcggcggcaccaaactggaaattaaaggcggcaaaggcagcggcggcaaaggcaccggcggca
aaggcagcggcggcaaaggcagcgaagtgaaactggtggaaagcggcggcgatctggtgcagccgggcggcagcctgaa
actgagctgcgcggtgcattgcgatcgcaccagcggctttacctttagcgattattatatgtattgggtgcgccagacc
ccggaaaaacgcctggaatgggtggcgtatattagcagcgtgcattgcgatcgcggcggcggcagcaccttttatccag
ataccgtgaaaggccgctttaccattagccgcgataacgcgaaaaacaccctgtatctacagatgagccgcctgaaaag
cgatgataccgcgatgtattattgcgcgcgcgcgtatcatggcaactattttgattattggggccagggcaccaccctg
accgtgagcagctccggtcaccaccaccaccaccactgatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgctcaccttcgggtgg
gcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagtcca
taagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctac
tagagaggggagagagccctgccatgaacgcgtctagcgaacgtgaccgttctctgtttggtgtgctgcgtcagttccg
ccgctccttttggtctgttggcatcttcagcgcggtcatcaatgttctgatgctggcacctagcgtctacatgctccag
gtttacgaccgtgtcctcgccagcggcaacggtattactctgctgatgctcacgctcctgatggcgggtctgtgcgcct
tcatgggtgctctcgaatgggttcgcagcctgctggtagttcgtctcggtactcgcatcgacctcgcgctcaatcaaga
cgtttttaacgcggctttcgcgcgtaatctggaagctggtgatggccgtgccggtctggcgctgaccgacctcaccctg
ctccgtcaattcatcacgggtaatgcactgttcgcgttcttcgacgttccgtggttcccgctctttctcctcgtactgt
tcctgctgcacccttggctcggcatgctcgcactgggtggtacggttgtacctggtggcgtaggcctcgcagaaccggc
gtctgaccagtctaccgcgggtggctctaatcaacaaagccaacaagccactcatctcgcggatgcgcagctccgtaat
gcggacgttatcgaagcgatgggtatgctcggtaacctccgtcgtcgctggctggcgcgtcactaccgctttatctccc
tccaaaatctcgcttccgagcgcgcagccgccgttggtggtgcgtccaaatactctcgtattgcactccaaagcctcat
gctgggcctcggtgcgctcctcgcgatcgacggcaaaatcacgccgggtatgatgattgcgggctctatcctcgtcggt
cgtgttctgtccccgattgaccagctgatcggtgtttggaaacagtggtcttctgcccgcattgcgtggcagcgcctga
cccgtctgattgccgcatatccgccacgccctgcggctatggctctcccagcgccggaaggccacctgtccgttgagca
agtttctctgcgtaccgcacaaggcaatactcgcctgcaaaacatccatttctccctgcaagcgggtgagaccctggtt
atcctgggcgcctctggttctggcaaatcttctctcgctcgtctgctcgtgggtgcacaatcccctactcaaggtaaag
ttcgcctcgatggtgcggacctgaaccaagttgacaaaaacaccttcggtccgaccattggttacctcccgcaggacgt
tcaactgttcaaaggtagcctggcggaaaacattgcccgtttcggcgacgccgacccggaaaaggttgttgcggcagct
aagctcgccggtgttcacgaactcatcctgtctctgcctaatggttacgacactgagctgggtgacggcggtggcggcc
tgtctggtggtcaacgtcagcgtatcggtctcgcacgcgcaatgtacggcgacccgtgtctcctcattctggacgaacc
gaacgcatctctggactctgagggtgaccaagcactcatgcaggctattgtggcgctccagaaacgtggtgcaaccgtt
gtactcattacgcaccgtccggcactcaccaccctcgctcagaaaatcctgatcctccacgagggtcaacagcaacgta
tgggcctggcccgtgacgttctgacggaactccagcagcgctccgcggcaaaccaggcgcgtatgaacccgaccgcagc
aatgccacagtaataatactagagagcagggatctcgtgatgactggtatggatattaccactcaggacgaactcaacg
aagctgccatgcgtgaccgtgcgagccgtgacgaagaacgtgcgctccgtctgggttggtggctcgtcctcgcgggttt
cggtggtttcctcctgtgggcgctcctggctccactggacaagggtgttgcggttcagggtaacgttgtagtttccggt
aaccgtaaagttatccagcacatgcaaggcggtattgttgaccgcatccaagttaaggacggcgaccgtgttgccgctg
gtcaggttctgctcactctcaatgcagtcgacgctcgtaccacctccgagggcctgggttctcagtacgatcagctcat
cgcgcgtgaagcgcgcctcctcgccgagcagcgtaaccagtcttctctcgctgcaacgccacgcctgacgcaggcacgc
cagcgtccggaaatggcggcgattatcgccctccaggaagacctgctgcgctctcgtcagcagtctctcaaactggaaa
ttgacggtgtccgtgcatctatcgatggtctggaaacttctctgggtgctctgcaaaaagtgatgtcttctaaacagtc
tgaacaagcgactctgtctcaacagctgcaaggtctgcgtccgctggccgcagacaactacgttccgcgtaacaagatg
ctcgaaaccgaacgtctcttcgcccaggtttctggcgagctggcccagacctctggtgaagttggtcgtacccgtcgtg
acattcaacaacagaaactccgcattgcgcagcgccagcaggagtacgacaaagaagtcaactctgaactgtctgacgt
tcaggccaaactgaacgaagtaatctctcagcgtgaaaaggcggacttcaatctcgctaacgtgcaggttcgtgcgccg
gtggcgggcactgtggtggatatgaaaatcttcaccgaaggtggcgtaattgctccgggtcaagttatgatggacattg
ttccggaggatcagccactgctggtagatggccgtatcccggttgagatggttgacaaagtatggagcggtctgccggt
cgaactgcaatttactgcgttttcccaatctactacgccgcgtgtccctggtactgtaaccctgctgagcgcggatcgc
ctggtggacgaaaaagatggcaccccttactatggcctccgtattcaggtgtctgaggaaggtaaacgtagcctccacg
gcctggagattaaaccgggtatgcctgtccaaggttttgtgcgtacgggcgaacgctctttcatcaactacctcttcaa
accgctcatggaccgtatgcacctcgcactgaccgaagaataatactagagagcggaaggaagcaatgcgccgtaaagc
agttctgctcactgtggttctgagcctgtctggtggtagcgcccaagcgatgggtctgctggacgcgtgggaactcgcg
ctccgcaatgatgcgcaactgcgtgcggcaggtttcgaacgtgacgcgggtcaagaggaagttgcgattggtcgcgcag
gcctcctgccgagcctgcaatacacgtacggtgcgaactactcccactccaaagttacccagcgcgatcgtaccctgaa
caacaccacgaaacgtgattatgataactacgtttctacgctgaccctgcgtcagccactcctggactacgcggcatgg
gcgcgttatcagcaaggcgtaactcgtaaactgatggcggaccagcgttttcgcgaccgttcccaggatctgatggttc
gtctgtaccagtcctggagcgaagccctgctggcgcaggaaaagctgatgctcctcgacgctcagcgtcgtgcatacca
ggaacagctcgcactgaatcgccgtctcctggctgccggtgagggcacgcaaaccgacctgcgtgagaccgaagcacgt
tataccgtcaccgaggcgcagcgtatcgaacaggaggacaccctcgatgccgccatgactgacctggaaaacatgatgg
gctctcctctccagatccaagacctgtctccactggctctcgacactctcccggataacgtaacggaaaaccgtagcct
ctctcagtggcgtgaactcacggtacgtcacaacgccaaactggcagtacaacgtgagaacgtggattactctcgctac
gaaatcgagcgcaaccgtgcgggccacctcccgaccctggacctggttgcctctacccgtaattccctctccgagagcg
aatacaactacaatcaaaaatacgacacccagaccgtaggtctgcaagttcgtgttcctctctactctggcggtgcggt
ttctgcgtctatgcgccaagcggcagctgagtatcaacagtcccaggcggaactggacaatcagactcgtcagaccttc
gcggagctgcgtcgccagttcaacctgtgcgcaaacggtgctgcgaaaatccgtgcttggcagatgtctgtagctgctg
ccgaagaggccattcgtgcgacccgtcagtctgttgcgggtggtgaacgtattaacctggatgtgctcatggctgagca
ggaatggtataatgcccgtcgtgagctgacggaggttaagtaccgttggctgcaagcgtggctcaatctgcgctacacg
gctggtactctcaacgaacaagatatgatgcagctggcagcctggttccagtctgcgccggttatcaataaaaccggca
tcaatgctgccacgggcaacaagaccaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagaaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaacaaac
agacaatctggtctgtttgtattattactagagtcacacaggaaacctactagatgtcctacgactacgagaaaaccag
cctcaccctctaccgggcggtattcaaggccaactacgacggcgacgtcggtcgctacctgcatcccgacaaggaactc
gccgaggctgcggaagtcgccccgctgctgcatccgaccttcgacagccccaacacccctggcgtccccgcccgcgcgc
cggacatcgtcgccggccgcgacggcctctacgccccggacaccggcggcacctcggtgttcgaccgcgccggcgtgct
gcgccgcgccgacggcgacttcgtgatacccgacggcaccgacatcccgccggaccttaaggtgaagcaggacagctac
aacaagcgcctgcaagccacccactacaccatcatgccggccaagccgatgtaccgggaggtcctcatgggccaactgg
acaacttcgtgcgcaacgccatccgccgccaatgggaaaaagcccgcgggctctagtactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata