LOCUS BBa_K950010 6018 bp DNA linear 22-Nov-2024 DEFINITION pRS315 shuttle vector ACCESSION BBa_K950010 VERSION BBa_K950010.1 FEATURES Location/Qualifiers CDS 663..1757 /label=LEU2 beta-isopropylmalate misc_feature 2698..2797 /label=F1 origin of replication CDS 2931..3268 /label=lacZ promoter 3091..3116 /label=T7 promoter misc_feature 3124..3225 /label=mcs misc_feature 3148..3153 /label=XbaI misc_feature 3154..3159 /label=SpeI promoter 3243..3262 /label=T3 promoter misc_feature 3686..3686 /label=PBM1 origin of replication CDS 4444..5304 /label=bla beta-lactamase, ampR ampicillin ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata 1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg 1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 2341 atgtaattgt tccgattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 2401 gaagttctcc tcgagggtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 3001 cagctggcga aggggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 3061 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa 3121 ttggagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcaggaa 3181 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccagcttttg 3241 ttccctttag tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt 3301 gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca taaagtgtaa 3361 agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc 3421 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 3481 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 3541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 3601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 3661 taaaaaggcc gcgttgctgg cgtttttcca taggctcggc ccccctgacg agcatcacaa 3721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 3781 cccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 3841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct 3901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 3961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 4021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 4081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 4141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 4201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 4261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 4321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 4381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 4441 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 4501 catagttgcc tgactgcccg tcgtgtagat aactacgata cgggagggct taccatctgg 4561 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 4621 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 4681 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 4741 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 4801 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgaaaaaa 4861 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 4921 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 4981 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 5041 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 5101 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 5161 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 5221 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 5281 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 5341 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 5401 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac gtgctataaa 5461 aataattata atttaaattt tttaatataa atatataaat taaaaataga aagtaaaaaa 5521 agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct agggggatcg 5581 ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg ccgaattgtt 5641 tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat tttacttatc 5701 gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat atatgtaaag 5761 tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca ttccgtaact 5821 cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa taaataaaca 5881 cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt aagttacagg 5941 caagcgatcc gtcctaagaa accattatta tcatgacatt aacctataaa aataggcgta 6001 tcacgaggcc ctttcgtc //