BBa_K952000
1
BBa_K952000
Phytochrome FphA - first 753 amino acids (Frieburg)
2012-09-26T11:00:00Z
2015-05-08T01:13:49Z
This part comes from the fungus Aspergillus nidulans, although it has been codon optimized for expression in E. coli. See http://www.ncbi.nlm.nih.gov/nuccore/167987798
Sensory domain of phytochrome FphA which has been codon optimized for expression in E. coli and conforms to RFC25 (Freiburg Standard) in order to allow for the fusion of other protein domains N- and C- terminally.
Note that in order for the sensor to function, FphA must bind a chromophore (biliverdin IXalpha (BV) acts as its natural chromophore). The chromophore can either be externally added to the protein, or can be endogenously created through expression of heme oxygenase.
Illumination with saturated red light (636 nm) results in formation of the Pfr form of the phytochrome (Amax 754nm), which can be converted back into the ground Pr form (Amax 707 nm) through illumination with far-red light (750 nm) (Brandt,S., Frankenberg-Dinkel,N., et. al. (2008) J. Biol. Chem. 283 (50), 34605-34614)
false
false
_1220_
0
13327
9
It's complicated
false
Cut sites had to be removed from the source gene in order to comply with Biobrick standards.
false
Steven Neuhaus
annotation2203420
1
C195, biliverdin IX􏰃 binding residue
range2203420
1
583
585
BBa_K952000_sequence
1
atgagcgagctgccgagccgtagcattagcccgcgtgatccgagcccgggtgaaaccccgggtcgcgatccgagcaccccgagcaccgatgcgggcgtgggttatagcgcgagccaggatgcgccgagctttggcgcgtatgatcgtgtgtatccgattcgtagcctggtgagcctggaaccgccagcgaccagcgaaccgagcagcaacaaaagcaaatctccgctgagcccgaccagcggtgcgcgtcagtttagcattattgatggccatacctggacccgtctgcgtagcgatagccgtgcgaacagcaccgattatagcggcggcaccggcctgagcccggaaagcagcgaagcgccgagcagccagcgtatgagcgatagcagcagcgcgcgtccgccgagcaataccaccggcctgcgtcgtggcgatgatcataccacctttaccccgagcagcgaagatagccatccgcaggtgcaggaaccgtatgagctgatgaccacccgttttcgtcatgtggtgaccgatgatggccatgcggtgattaccggccgtaccgtggatagctttaaagcgtgcgaagatgaaccgattcatattccgggtgcgattcagagctttggtgcgctggttgcggtgcgtgaagaaccgggcgaacaaatggtggtgcgtattgtgagcgaaaacagccaggatattctgggctattctccgaacgacctgtttagcctgccgaccctgtgcgatatttttccggaagatcaggcggataactttctggatcatgtggatttcgtgaaagaagaaggctacgatccgagcgtggatggcccggaagtgtttattctgaccgtgaaccagccgaacggcagcaccattcgtgtgtggtgcgcgattcataccaatccggcgctgaacggcctggtgatttgcgaatttgagctggaagatgatcatgtgaatccgctgaccagcagcggccatattagcccggcggtgccgaccaataccctggatgtggtgccgaccattgaacaaatggcgggtagcaccattaccattaaccagccgctgcgtgtgctgcgtcgtgcgcgtcgccgtaaaggtgaagcggcggcgatggaagtgtttagcattgtgagccagattcaggaacagctggcacgcgcggataacatggaagcgctgctggataccacctctggcattgtgaaagagctgaccggctttcatcgtattctggtgtatcagtttgatagcgagttcaacggcaaagtggtgagcgagctggttgatccgaccatgagcattgacctgtttaaaggcctgcattttccggcagcggatattccgaaacaggcgcgtgatctgtatcgtattaacaaagtgcgtctgctgtacgatcgtgatcatgtgaccgcgcgtctggtgtgtcgtgcgctggaagatctggaaaccccgctggatatgacccatgcgtatctgcgtgcgatgagcccgattcatatcaaatatctggccaacatgcagattcgtagcagcatgagcattagcattaacagcatgaacgatctgtggggcctgattagctgccattcttatggcccgcgtggcatgcgtgtgagctttccgattcgtaaaatgtgccgtctgattggcgataccgtgagccgtaacattgaacgtctgagctatgcgagccgtctccaggcgcgtaaactgattaacaccgtgccgaccgatgcgaatccgagcggctatattgtggcgagcagcgatgatctgctgcgtctggtggatgcggattatggcgcgctgtgcattcgtggcgaagtgaaaattctggccaaaagcccgcagagccaggaaatgctggccctgctggaatatctgaaagtgcgcaaatataacagcgtgctgaccagcaaccatatcgtgaaagatttccaggatctgaactatccgccgggttttaaagatattagcggcctgctgtatgtgccgctgagcaccgatggcctggattttattgtgttttttcgtcgtggccagctgaccgaagtgaaatggggcggcaatccgaacgaagcgaaatttaccgaaggccatctggaaccgcgtaaaagctttcagacctggcgtgaaaccgtgctggatcgttgccgtgattggaccgaaagcgaagtggataccgcggcggtgctgtgcctggtgtatggcaaatttattaaagtgtggcgtcagcaggaagcg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z