LOCUS       BBa_K952002             3317 bp    DNA     linear       22-Dec-2024
DEFINITION  B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette
ACCESSION   BBa_K952002
VERSION     BBa_K952002.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1149
                     /label=YF1
     CDS             19..1149
                     /label=BBa_K592004
     misc_feature    1162..1165
                     /label=conserved
     RBS             1158..1169
                     /label=BBa_B0034
     CDS             1176..1796
                     /label=FixJ
     CDS             1176..1796
                     /label=BBa_K592005
     misc_feature    1..1796
                     /label=BBa_K592016
     promoter        1805..2054
                     /label=FixK2 promoter
     promoter        1805..2054
                     /label=BBa_K592006
     CDS             2061..3317
                     /label=BBa_K124017
ORIGIN
        1 aaagaggaga aatactagat ggctagtttt caatcatttg ggataccagg acagctggaa
       61 gtcatcaaaa aagcacttga tcacgtgcga gtcggtgtgg taattacaga tcccgcactt
      121 gaagataatc ctattgtcta cgtaaatcaa ggctttgttc aaatgaccgg ctacgagacc
      181 gaggaaattt taggaaagaa ctgtcgcttc ttacagggga aacacacaga tccggcagaa
      241 gtggacaaca tcagaaccgc tttacaaaat aaagaaccgg tcaccgttca gatccaaaac
      301 tacaaaaaag acggaacgat gttctggaat gaattaaata ttgatccaat ggaaatagag
      361 gataaaacgt attttgtcgg cattcagaat gatatcaccg agcaccagca gacccaggcg
      421 cgtctccagg aactgcaatc cgagctcgtc cacgtctcca ggctgagcgc catgggcgaa
      481 atggcgtccg cgctcgcgca cgagctcaac cagccgctgg cggcgatcag caactacatg
      541 aagggctcgc ggcggctgct tgccggcagc agtgatccga acacaccgaa ggtcgaaagc
      601 gccctggacc gcgccgccga gcaggcgctg cgcgccggcc agatcatccg gcgcctgcgc
      661 gacttcgttg cccgcggcga atcggagaag cgggtcgaga gtctctccaa gctgatcgag
      721 gaggccggcg cgctcgggct tgccggcgcg cgcgagcaga acgtgcagct ccgcttcagt
      781 ctcgatccgg gcgccgatct cgttctcgcc gaccgggtgc agatccagca ggtcctggtc
      841 aacctgttcc gcaacgcgct ggaagcgatg gctcagtcgc agcgacgcga gctcgtcgtc
      901 accaacaccc ccgccgccga cgacatgatc gaggtcgaag tgtccgacac cggcagcggt
      961 ttccaggacg acgtcattcc gaacctgttt cagactttct tcaccaccaa ggacaccggc
     1021 atgggcgtgg gactgtccat cagccgctcg atcatcgaag ctcacggcgg gcgcatgtgg
     1081 gccgagagca acgcatcggg cggggcgacc ttccgcttca ccctcccggc agccgacgag
     1141 aattaataat actagagaaa gaggagaaat actagatgac gaccaaggga catatctacg
     1201 tcatcgacga cgacgcggcg atgcgggatt cgctgaattt cctgctggat tctgccggct
     1261 tcggcgtcac gctgtttgac gacgcgcaag cctttctcga cgccctgccg ggtctctcct
     1321 tcggctgcgt cgtctccgac gtgcgcatgc cgggccttga cggcatcgag ctgttgaagc
     1381 ggatgaaggc gcagcaaagc ccctttccga tcctcatcat gaccggtcac ggcgacgtgc
     1441 cgctcgcggt cgaggcgatg aagttagggg cggtcgactt tctggaaaag cctttcgagg
     1501 acgaccgcct caccgccatg atcgaatcgg cgatccgcca ggccgagccg gccgccaaga
     1561 gcgaggccgt cgcgcaggat atcgccgccc gcgtcgcctc gttgagcccc agggagcgcc
     1621 aggtcatgga agggctgatc gccggccttt ccaacaagct gatcgcccgc gagtacgaca
     1681 tcagcccgcg caccatcgag gtgtatcggg ccaacgtcat gaccaagatg caggccaaca
     1741 gcctttcgga gctggttcgc ctcgcgatgc gcgccggcat gctcaacgat taataatact
     1801 agaggccgga gccgattatc cgcacccgtc cttggtcttg gacacactcg ctcgcgatcc
     1861 gaagccgcct ttcaaagata ctccgcagtg aaacgcatct tttaagcgcg acttgtcgcc
     1921 ttcgcgcagc agaacactcg tatggcactc aatccatgat cgcttcgttc gctccgacgc
     1981 gcgttgcggg gcccccctcg accggatgac aacacattgc gtcacctcaa ctgctgcgcg
     2041 cgcactgcgt cacatactag atgccagaaa aacatgacct gttggccgcc attctcgcgg
     2101 caaaggaaca aggcatcggg gcaatccttg cgtttgcaat ggcgtacctt cgcggcagat
     2161 ataatggcgg tgcgtttaca aaaacagtaa tcgacgcaac gatgtgcgcc attatcgcct
     2221 ggttcattcg tgaccttctc gacttcgccg gactaagtag caatctcgct tatataacga
     2281 gcgtgtttat cggctacatc ggtactgact cgattggttc gcttatcaaa cgcttcgctg
     2341 ctaaaaaagc cggagtagaa gatggtagaa atcaataaat ggtagaaatc aataatcaac
     2401 gtaaggcgtt cctcgatatg ctggcgtggt cggagggaac tgataacgga cgtcagaaaa
     2461 ccagaaatca tggttatgac gtcattgtag gcggagagct atttactgat tactccgatc
     2521 accctcgcaa acttgtcacg ctaaacccaa aactcaaatc aacaggcgcc ggacgctacc
     2581 agcttctttc ccgttggtgg gatgcctacc gcaagcagct tggcctgaaa gacttctctc
     2641 cgaaaagtca ggacgctgtg gcattgcagc agattaagga gcgtggcgct ttacctatga
     2701 ttgatcgtgg tgatatccgt caggcaatcg accgttgcag caatatctgg gcttcactgc
     2761 cgggcgctgg ttatggtcag ttcgagcata aggctgacag cctgattgca aaattcaaag
     2821 aagcgggcgg aacggtcaga gagattgatg tatgaatgag cagagtcacc gcgattatct
     2881 ccgctctggt tatctgcatc atcgtctgcc tgtcatgggc tgttaatcat taccgtgata
     2941 acgccattac ctacaaagcc cagcgcgaca aaaatgccag agaactgaag ctggcgaacg
     3001 cggcaattac tgacatgcag atgcgtcagc gtgatgttgc tgcgctcgat gcaaaataca
     3061 cgaaggagtt agctgatgct aaagctgaaa atgatgctct gcgtgatgat gttgccgctg
     3121 gtcgtcgtcg gttgcacatc aaagcagtct gtcagtcagt gcgtgaagcc accaccgcct
     3181 ccggcgtgga taatgcagcc tccccccgac tggcagacac cgctgaacgg gattatttca
     3241 ccctcagaga gaggctgatc actatgcaaa aacaactgga aggaacccag aagtatatta
     3301 atgagcagtg cagatag
//