LOCUS       BBa_K952005             2024 bp    DNA     linear       29-Nov-2024
DEFINITION  IPTG inducible promoter-B0034-YF1-B0034-FixJ
ACCESSION   BBa_K952005
VERSION     BBa_K952005.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    233..236
                     /label=conserved
     RBS             229..240
                     /label=BBa_B0034
     CDS             247..1377
                     /label=YF1
     CDS             247..1377
                     /label=BBa_K592004
     misc_feature    1390..1393
                     /label=conserved
     RBS             1386..1397
                     /label=BBa_B0034
     CDS             1404..2024
                     /label=FixJ
     CDS             1404..2024
                     /label=BBa_K592005
     misc_feature    229..2024
                     /label=BBa_K592016
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagaa agaggagaaa
      241 tactagatgg ctagttttca atcatttggg ataccaggac agctggaagt catcaaaaaa
      301 gcacttgatc acgtgcgagt cggtgtggta attacagatc ccgcacttga agataatcct
      361 attgtctacg taaatcaagg ctttgttcaa atgaccggct acgagaccga ggaaatttta
      421 ggaaagaact gtcgcttctt acaggggaaa cacacagatc cggcagaagt ggacaacatc
      481 agaaccgctt tacaaaataa agaaccggtc accgttcaga tccaaaacta caaaaaagac
      541 ggaacgatgt tctggaatga attaaatatt gatccaatgg aaatagagga taaaacgtat
      601 tttgtcggca ttcagaatga tatcaccgag caccagcaga cccaggcgcg tctccaggaa
      661 ctgcaatccg agctcgtcca cgtctccagg ctgagcgcca tgggcgaaat ggcgtccgcg
      721 ctcgcgcacg agctcaacca gccgctggcg gcgatcagca actacatgaa gggctcgcgg
      781 cggctgcttg ccggcagcag tgatccgaac acaccgaagg tcgaaagcgc cctggaccgc
      841 gccgccgagc aggcgctgcg cgccggccag atcatccggc gcctgcgcga cttcgttgcc
      901 cgcggcgaat cggagaagcg ggtcgagagt ctctccaagc tgatcgagga ggccggcgcg
      961 ctcgggcttg ccggcgcgcg cgagcagaac gtgcagctcc gcttcagtct cgatccgggc
     1021 gccgatctcg ttctcgccga ccgggtgcag atccagcagg tcctggtcaa cctgttccgc
     1081 aacgcgctgg aagcgatggc tcagtcgcag cgacgcgagc tcgtcgtcac caacaccccc
     1141 gccgccgacg acatgatcga ggtcgaagtg tccgacaccg gcagcggttt ccaggacgac
     1201 gtcattccga acctgtttca gactttcttc accaccaagg acaccggcat gggcgtggga
     1261 ctgtccatca gccgctcgat catcgaagct cacggcgggc gcatgtgggc cgagagcaac
     1321 gcatcgggcg gggcgacctt ccgcttcacc ctcccggcag ccgacgagaa ttaataatac
     1381 tagagaaaga ggagaaatac tagatgacga ccaagggaca tatctacgtc atcgacgacg
     1441 acgcggcgat gcgggattcg ctgaatttcc tgctggattc tgccggcttc ggcgtcacgc
     1501 tgtttgacga cgcgcaagcc tttctcgacg ccctgccggg tctctccttc ggctgcgtcg
     1561 tctccgacgt gcgcatgccg ggccttgacg gcatcgagct gttgaagcgg atgaaggcgc
     1621 agcaaagccc ctttccgatc ctcatcatga ccggtcacgg cgacgtgccg ctcgcggtcg
     1681 aggcgatgaa gttaggggcg gtcgactttc tggaaaagcc tttcgaggac gaccgcctca
     1741 ccgccatgat cgaatcggcg atccgccagg ccgagccggc cgccaagagc gaggccgtcg
     1801 cgcaggatat cgccgcccgc gtcgcctcgt tgagccccag ggagcgccag gtcatggaag
     1861 ggctgatcgc cggcctttcc aacaagctga tcgcccgcga gtacgacatc agcccgcgca
     1921 ccatcgaggt gtatcgggcc aacgtcatga ccaagatgca ggccaacagc ctttcggagc
     1981 tggttcgcct cgcgatgcgc gccggcatgc tcaacgatta ataa
//