LOCUS       BBa_K952007             3545 bp    DNA     linear       10-Dec-2025
DEFINITION  IPTG induc. promoter-B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette
ACCESSION   BBa_K952007
VERSION     BBa_K952007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    233..236
                     /label=conserved
     RBS             229..240
                     /label=BBa_B0034
     CDS             247..1377
                     /label=YF1
     CDS             247..1377
                     /label=BBa_K592004
     misc_feature    1390..1393
                     /label=conserved
     RBS             1386..1397
                     /label=BBa_B0034
     CDS             1404..2024
                     /label=FixJ
     CDS             1404..2024
                     /label=BBa_K592005
     misc_feature    229..2024
                     /label=BBa_K592016
     promoter        2033..2282
                     /label=FixK2 promoter
     promoter        2033..2282
                     /label=BBa_K592006
     CDS             2289..3545
                     /label=BBa_K124017
     misc_feature    229..3545
                     /label=BBa_K952002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagaa agaggagaaa
      241 tactagatgg ctagttttca atcatttggg ataccaggac agctggaagt catcaaaaaa
      301 gcacttgatc acgtgcgagt cggtgtggta attacagatc ccgcacttga agataatcct
      361 attgtctacg taaatcaagg ctttgttcaa atgaccggct acgagaccga ggaaatttta
      421 ggaaagaact gtcgcttctt acaggggaaa cacacagatc cggcagaagt ggacaacatc
      481 agaaccgctt tacaaaataa agaaccggtc accgttcaga tccaaaacta caaaaaagac
      541 ggaacgatgt tctggaatga attaaatatt gatccaatgg aaatagagga taaaacgtat
      601 tttgtcggca ttcagaatga tatcaccgag caccagcaga cccaggcgcg tctccaggaa
      661 ctgcaatccg agctcgtcca cgtctccagg ctgagcgcca tgggcgaaat ggcgtccgcg
      721 ctcgcgcacg agctcaacca gccgctggcg gcgatcagca actacatgaa gggctcgcgg
      781 cggctgcttg ccggcagcag tgatccgaac acaccgaagg tcgaaagcgc cctggaccgc
      841 gccgccgagc aggcgctgcg cgccggccag atcatccggc gcctgcgcga cttcgttgcc
      901 cgcggcgaat cggagaagcg ggtcgagagt ctctccaagc tgatcgagga ggccggcgcg
      961 ctcgggcttg ccggcgcgcg cgagcagaac gtgcagctcc gcttcagtct cgatccgggc
     1021 gccgatctcg ttctcgccga ccgggtgcag atccagcagg tcctggtcaa cctgttccgc
     1081 aacgcgctgg aagcgatggc tcagtcgcag cgacgcgagc tcgtcgtcac caacaccccc
     1141 gccgccgacg acatgatcga ggtcgaagtg tccgacaccg gcagcggttt ccaggacgac
     1201 gtcattccga acctgtttca gactttcttc accaccaagg acaccggcat gggcgtggga
     1261 ctgtccatca gccgctcgat catcgaagct cacggcgggc gcatgtgggc cgagagcaac
     1321 gcatcgggcg gggcgacctt ccgcttcacc ctcccggcag ccgacgagaa ttaataatac
     1381 tagagaaaga ggagaaatac tagatgacga ccaagggaca tatctacgtc atcgacgacg
     1441 acgcggcgat gcgggattcg ctgaatttcc tgctggattc tgccggcttc ggcgtcacgc
     1501 tgtttgacga cgcgcaagcc tttctcgacg ccctgccggg tctctccttc ggctgcgtcg
     1561 tctccgacgt gcgcatgccg ggccttgacg gcatcgagct gttgaagcgg atgaaggcgc
     1621 agcaaagccc ctttccgatc ctcatcatga ccggtcacgg cgacgtgccg ctcgcggtcg
     1681 aggcgatgaa gttaggggcg gtcgactttc tggaaaagcc tttcgaggac gaccgcctca
     1741 ccgccatgat cgaatcggcg atccgccagg ccgagccggc cgccaagagc gaggccgtcg
     1801 cgcaggatat cgccgcccgc gtcgcctcgt tgagccccag ggagcgccag gtcatggaag
     1861 ggctgatcgc cggcctttcc aacaagctga tcgcccgcga gtacgacatc agcccgcgca
     1921 ccatcgaggt gtatcgggcc aacgtcatga ccaagatgca ggccaacagc ctttcggagc
     1981 tggttcgcct cgcgatgcgc gccggcatgc tcaacgatta ataatactag aggccggagc
     2041 cgattatccg cacccgtcct tggtcttgga cacactcgct cgcgatccga agccgccttt
     2101 caaagatact ccgcagtgaa acgcatcttt taagcgcgac ttgtcgcctt cgcgcagcag
     2161 aacactcgta tggcactcaa tccatgatcg cttcgttcgc tccgacgcgc gttgcggggc
     2221 ccccctcgac cggatgacaa cacattgcgt cacctcaact gctgcgcgcg cactgcgtca
     2281 catactagat gccagaaaaa catgacctgt tggccgccat tctcgcggca aaggaacaag
     2341 gcatcggggc aatccttgcg tttgcaatgg cgtaccttcg cggcagatat aatggcggtg
     2401 cgtttacaaa aacagtaatc gacgcaacga tgtgcgccat tatcgcctgg ttcattcgtg
     2461 accttctcga cttcgccgga ctaagtagca atctcgctta tataacgagc gtgtttatcg
     2521 gctacatcgg tactgactcg attggttcgc ttatcaaacg cttcgctgct aaaaaagccg
     2581 gagtagaaga tggtagaaat caataaatgg tagaaatcaa taatcaacgt aaggcgttcc
     2641 tcgatatgct ggcgtggtcg gagggaactg ataacggacg tcagaaaacc agaaatcatg
     2701 gttatgacgt cattgtaggc ggagagctat ttactgatta ctccgatcac cctcgcaaac
     2761 ttgtcacgct aaacccaaaa ctcaaatcaa caggcgccgg acgctaccag cttctttccc
     2821 gttggtggga tgcctaccgc aagcagcttg gcctgaaaga cttctctccg aaaagtcagg
     2881 acgctgtggc attgcagcag attaaggagc gtggcgcttt acctatgatt gatcgtggtg
     2941 atatccgtca ggcaatcgac cgttgcagca atatctgggc ttcactgccg ggcgctggtt
     3001 atggtcagtt cgagcataag gctgacagcc tgattgcaaa attcaaagaa gcgggcggaa
     3061 cggtcagaga gattgatgta tgaatgagca gagtcaccgc gattatctcc gctctggtta
     3121 tctgcatcat cgtctgcctg tcatgggctg ttaatcatta ccgtgataac gccattacct
     3181 acaaagccca gcgcgacaaa aatgccagag aactgaagct ggcgaacgcg gcaattactg
     3241 acatgcagat gcgtcagcgt gatgttgctg cgctcgatgc aaaatacacg aaggagttag
     3301 ctgatgctaa agctgaaaat gatgctctgc gtgatgatgt tgccgctggt cgtcgtcggt
     3361 tgcacatcaa agcagtctgt cagtcagtgc gtgaagccac caccgcctcc ggcgtggata
     3421 atgcagcctc cccccgactg gcagacaccg ctgaacggga ttatttcacc ctcagagaga
     3481 ggctgatcac tatgcaaaaa caactggaag gaacccagaa gtatattaat gagcagtgca
     3541 gatag
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