BBa_K952007
type: ComponentDefinition
persistent identity: BBa K952007
display id: BBa_K952007
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG induc. promoter-B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:49Z
mutable provenance: Part BBa_K952002 was a composite part designed by our team, see BBa_K952002 for further information. BBa_J04500 was obtained from the 2012 kit.
owned by: james
owned by: myers
mutable description: This part has the IPTG inducible promoter (BBa_J23102) upstream and BBa_K952002 downstream. BBa_K952002 originally did not have a promoter. This can be used immediately in a cell to cause lysis/cell death when a cell is exposed to blue-light and IPTG.
discontinued: false
dominant: false
experience: experience/None
group u list: _1220_
m user id: 0
owner id: 13332
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: N/A
star: false
creator: Saimon Sharif
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K952002
component: BBa_J04500
sequence annotation: BBa_J04500
sequence annotation: BBa_K952002
sequence: https://synbiohub.org/public/igem/BBa_K952007_sequence/1

BBa_K952002
type: Component
persistent identity: component2205404
display id: component2205404
version: 1
top level: BBa_K952007
definition: BBa_K952002
access: public

BBa_J04500
type: Component
persistent identity: component2205391
display id: component2205391
version: 1
top level: BBa_K952007
definition: BBa_J04500
access: public

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2205391
display id: annotation2205391
version: 1
top level: BBa_K952007
location: https://synbiohub.org/public/igem/BBa_K952007/annotation2205391/range2205391/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K952007/annotation2205391/range2205391/1
type: Range
persistent identity: range2205391
display id: range2205391
version: 1
top level: BBa_K952007
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K952002
type: SequenceAnnotation
persistent identity: annotation2205404
display id: annotation2205404
version: 1
top level: BBa_K952007
location: https://synbiohub.org/public/igem/BBa_K952007/annotation2205404/range2205404/1
component: BBa_K952002

https://synbiohub.org/public/igem/BBa_K952007/annotation2205404/range2205404/1
type: Range
persistent identity: range2205404
display id: range2205404
version: 1
top level: BBa_K952007
direction: direction/reverse
start: 229
end: 3545
orientation: inline

BBa_K952002
type: ComponentDefinition
persistent identity: BBa K952002
display id: BBa_K952002
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:49Z
mutable provenance: Part BBa_K952001 was a composite part designed by our team, see BBa_K952001 for further information. BBa_K592016 is available from the registry, but obtained directly from the Uppsala-Sweden 2011 team.
owned by: james
owned by: myers
mutable description: This part has BBa_K952001 downstream and BBa_K592016 upstream. This part causes cells to become blue-light sensitive; the cells will lyse when exposed to blue-light.
discontinued: false
dominant: false
experience: experience/None
group u list: _1220_
m user id: 0
owner id: 13332
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Saimon Sharif
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K124017
component: BBa_K592006
component: BBa_K592016
sequence annotation: BBa_K592016
sequence annotation: BBa_K592006
sequence annotation: BBa_K124017
sequence: https://synbiohub.org/public/igem/BBa_K952002_sequence/1

BBa_K124017
type: Component
persistent identity: component2205315
display id: component2205315
version: 1
top level: BBa_K952002
definition: BBa_K124017
access: public

BBa_K592006
type: Component
persistent identity: component2205314
display id: component2205314
version: 1
top level: BBa_K952002
definition: BBa_K592006
access: public

BBa_K592016
type: Component
persistent identity: component2205312
display id: component2205312
version: 1
top level: BBa_K952002
definition: BBa_K592016
access: public

BBa_K592016
type: SequenceAnnotation
persistent identity: annotation2205312
display id: annotation2205312
version: 1
top level: BBa_K952002
location: https://synbiohub.org/public/igem/BBa_K952002/annotation2205312/range2205312/1
component: BBa_K592016

https://synbiohub.org/public/igem/BBa_K952002/annotation2205312/range2205312/1
type: Range
persistent identity: range2205312
display id: range2205312
version: 1
top level: BBa_K952002
direction: direction/reverse
start: 1
end: 1796
orientation: inline

BBa_K592006
type: SequenceAnnotation
persistent identity: annotation2205314
display id: annotation2205314
version: 1
top level: BBa_K952002
location: https://synbiohub.org/public/igem/BBa_K952002/annotation2205314/range2205314/1
component: BBa_K592006

https://synbiohub.org/public/igem/BBa_K952002/annotation2205314/range2205314/1
type: Range
persistent identity: range2205314
display id: range2205314
version: 1
top level: BBa_K952002
direction: direction/reverse
start: 1805
end: 2054
orientation: inline

BBa_K124017
type: SequenceAnnotation
persistent identity: annotation2205315
display id: annotation2205315
version: 1
top level: BBa_K952002
location: https://synbiohub.org/public/igem/BBa_K952002/annotation2205315/range2205315/1
component: BBa_K124017

https://synbiohub.org/public/igem/BBa_K952002/annotation2205315/range2205315/1
type: Range
persistent identity: range2205315
display id: range2205315
version: 1
top level: BBa_K952002
direction: direction/reverse
start: 2061
end: 3317
orientation: inline

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K592006
type: ComponentDefinition
persistent identity: BBa K592006
display id: BBa_K592006
version: 1
was generated by: iGEM to SBOL conversion
description: FixK2 promoter
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: FixK2 promoter omes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.
discontinued: false
dominant: false
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
star: false
creator: Lei Sun
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: FixK2 promoter
sequence: https://synbiohub.org/public/igem/BBa_K592006_sequence/1

FixK2 promoter
type: SequenceAnnotation
persistent identity: annotation2130179
display id: annotation2130179
version: 1
top level: BBa_K592006
location: https://synbiohub.org/public/igem/BBa_K592006/annotation2130179/range2130179/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K592006/annotation2130179/range2130179/1
type: Range
persistent identity: range2130179
display id: range2130179
version: 1
top level: BBa_K592006
direction: direction/forward
start: 1
end: 250
orientation: inline

BBa_K592016
type: ComponentDefinition
persistent identity: BBa K592016
display id: BBa_K592016
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: The components that made up of YF1 fusion protein comes from Bradyrhizobium japonicum and Bacillus subtilis. FixJ comes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: YF1 works in conjunction with its response regulator, FixJ. This part consists of RBS-coupled YF1 and FixJ without promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This constructs Strong RBS to ensure good expression.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592004
component: BBa_B0034
component: BBa_K592005
sequence annotation: BBa_B0034
sequence annotation: BBa_K592004
sequence annotation: BBa_K592005
sequence: https://synbiohub.org/public/igem/BBa_K592016_sequence/1

BBa_K592004
type: Component
persistent identity: component2136335
display id: component2136335
version: 1
top level: BBa_K592016
definition: YF1
access: public

BBa_B0034
type: Component
persistent identity: component2136333
display id: component2136333
version: 1
top level: BBa_K592016
definition: BBa_B0034
access: public

BBa_K592005
type: Component
persistent identity: component2136339
display id: component2136339
version: 1
top level: BBa_K592016
definition: FixJ
access: public

BBa_B0034
type: Component
persistent identity: component2136337
display id: component2136337
version: 1
top level: BBa_K592016
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136333
display id: annotation2136333
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136333/range2136333/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592016/annotation2136333/range2136333/1
type: Range
persistent identity: range2136333
display id: range2136333
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K592004
type: SequenceAnnotation
persistent identity: annotation2136335
display id: annotation2136335
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136335/range2136335/1
component: BBa_K592004

https://synbiohub.org/public/igem/BBa_K592016/annotation2136335/range2136335/1
type: Range
persistent identity: range2136335
display id: range2136335
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 19
end: 1149
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136337
display id: annotation2136337
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136337/range2136337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592016/annotation2136337/range2136337/1
type: Range
persistent identity: range2136337
display id: range2136337
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1158
end: 1169
orientation: inline

BBa_K592005
type: SequenceAnnotation
persistent identity: annotation2136339
display id: annotation2136339
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136339/range2136339/1
component: BBa_K592005

https://synbiohub.org/public/igem/BBa_K592016/annotation2136339/range2136339/1
type: Range
persistent identity: range2136339
display id: range2136339
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1176
end: 1796
orientation: inline

BBa_K124017
type: ComponentDefinition
persistent identity: BBa K124017
display id: BBa_K124017
version: 1
was generated by: iGEM to SBOL conversion
description: Bacteriophage Lysis Cassette S105, R, and Rz
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: The DNA for this part was graciously donated to the Brown iGEM Team by Ry Young at Texas A&M University. The team then BioBricked the part for submission to the registry.
owned by: james
owned by: myers
mutable description: The gene cassette consists of the S105 protein (Holin), R protein (Endolysin), and Rz protein. When paired with an inducible promoter, the cassette is activated by the inducer and begins to produce the proteins which lyse the cell. This cassette contains the mutant S gene, which can be found individually as BBa_K124014. The S variation allows for faster lysis.
discontinued: false
dominant: false
experience: experience/None
group u list: _221_
m user id: 0
owner id: 3365
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None-
star: true
creator: Neil J. Parikh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K124017_sequence/1

YF1
type: ComponentDefinition
persistent identity: BBa K592004
display id: BBa_K592004
version: 1
was generated by: iGEM to SBOL conversion
description: YF1 blue-light sensor protein
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Ytva comes from the genome of Bacillus subtilis. FixL derives from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: YF1 is a fusion protein of YtvA (B.subtilis) and FixL(Br.japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. As shown in previous research, Illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo.
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence information was provided in Moglich and Moffat's article. The physical DNA was synthesized by GenScript. Codon-optimization may enhance its expression.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: YF1
sequence: https://synbiohub.org/public/igem/BBa_K592004_sequence/1

YF1
type: SequenceAnnotation
persistent identity: annotation2130111
display id: annotation2130111
version: 1
top level: YF1
location: https://synbiohub.org/public/igem/BBa_K592004/annotation2130111/range2130111/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K592004/annotation2130111/range2130111/1
type: Range
persistent identity: range2130111
display id: range2130111
version: 1
top level: YF1
direction: direction/forward
start: 1
end: 1131
orientation: inline

FixJ
type: ComponentDefinition
persistent identity: BBa K592005
display id: BBa_K592005
version: 1
was generated by: iGEM to SBOL conversion
description: FixJ, the response regulator to YF1
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: FixJ comes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
mutable description: FixJ comes from the genome of Bradyrhizobium japonicum. It's the wild-type response regulator to YF1, the blue light-sensing fusion protein.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence was information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: FixJ
sequence: https://synbiohub.org/public/igem/BBa_K592005_sequence/1

FixJ
type: SequenceAnnotation
persistent identity: annotation2130114
display id: annotation2130114
version: 1
top level: FixJ
location: https://synbiohub.org/public/igem/BBa_K592005/annotation2130114/range2130114/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K592005/annotation2130114/range2130114/1
type: Range
persistent identity: range2130114
display id: range2130114
version: 1
top level: FixJ
direction: direction/forward
start: 1
end: 621
orientation: inline

https://synbiohub.org/public/igem/BBa_K952007_sequence/1
type: Sequence
persistent identity: BBa K952007 sequence
display id: BBa_K952007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagaaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K952002_sequence/1
type: Sequence
persistent identity: BBa K952002 sequence
display id: BBa_K952002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataatactagaggccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592006_sequence/1
type: Sequence
persistent identity: BBa K592006 sequence
display id: BBa_K592006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccggatgacaacacattgcgtcacctcaactgctgcgcgcgcactgcgtcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592016_sequence/1
type: Sequence
persistent identity: BBa K592016 sequence
display id: BBa_K592016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K124017_sequence/1
type: Sequence
persistent identity: BBa K124017 sequence
display id: BBa_K124017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataaatggtagaaatcaataatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggcggagagctatttactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaacaggcgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccgaaaagtcaggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatcgaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattgcaaaattcaaagaagcgggcggaacggtcagagagattgatgtatgaatgagcagagtcaccgcgattatctccgctctggttatctgcatcatcgtctgcctgtcatgggctgttaatcattaccgtgataacgccattacctacaaagcccagcgcgacaaaaatgccagagaactgaagctggcgaacgcggcaattactgacatgcagatgcgtcagcgtgatgttgctgcgctcgatgcaaaatacacgaaggagttagctgatgctaaagctgaaaatgatgctctgcgtgatgatgttgccgctggtcgtcgtcggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccaccaccgcctccggcgtggataatgcagcctccccccgactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatgcaaaaacaactggaaggaacccagaagtatattaatgagcagtgcagatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592004_sequence/1
type: Sequence
persistent identity: BBa K592004 sequence
display id: BBa_K592004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592005_sequence/1
type: Sequence
persistent identity: BBa K592005 sequence
display id: BBa_K592005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z