BBa_K953002
1
BBa_K953002
Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)
2012-09-23T11:00:00Z
2015-05-08T01:13:49Z
Agrobacterium tumefaciens
Released HQ 2013
Agrobacterium tumefaciens bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light(620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time.
As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells.
false
false
_1221_
0
14275
9
In stock
true
Optimised for expression in E. coli
false
Daniel Winter
annotation2196369
1
Start Codon
range2196369
1
23
25
annotation2196371
1
Stop
range2196371
1
2231
2236
annotation2196372
1
BBa_K953002
range2196372
1
1
2236
annotation2196370
1
Bacteriophytochrome
range2196370
1
26
2230
annotation2196368
1
RBS
range2196368
1
1
22
BBa_K953002_sequence
1
ctttaagaaggagatatacataatgagttcacatacgccgaaactggatagttgcggcgcagagcccatccacattcccggtgctattcaggaacatggagcccttctggttctgtccgcgcgggaattttccgtggtgcaggcgagcgataatctcgccaactatattggtgtggacctgccaatcggggccgttgccaccgaagcaaacttgccgttcatctccgtactgtccgcctggtattccggcgaggagagcaatttccgatatgcctgggccgaaaagaaactcgatgtttcggcgcatcgatccgggaccttggttattcttgaggtggaaaaggcaggagtgggcgaatccgctgaaaagctgatgggcgaactcaccagtctggcgaaatatctgaacagtgcgccgtctctggaggacgcgctttttcggacagcgcaactggtgtcttctatcagcggtcatgaccgtacactgatctatgatttcggactggactggtctggtcatgttgtggcggaagcgggatctggtgctttgccgtcttatctcggtcttcgctttcctgcgggtgacatccctccgcaagcgcggcaactctatacaatcaatcgcctgcggatgattcctgatgttgattataagcctgttccgatcagaccggaggttaatgcggaaacaggagcggttctcgatatgagcttttctcaacttcgctctgtatctccggttcatctcgaatatatgcgcaatatgggtactgccgcttctatgtctgttagcatcgttgttaatggtgctctctggggcttgattgcctgtcaccacgcgacacctcattctgtatcattagcagttcgtgaagcctgtgattttgcagcgcagctcctgtcgatgcggatcgcgatggagcagagttcccaggatgcatcccgccgggtggagcttggccacattcaggcgcggctcttgaaaggcatggccgccgccgaaaaatgggttgatggcctgctgggaggcgaaggtgaaagagaagacctgctgaaacaggtgggcgcggatggcgcagcacttgtcctgggcgacgactatgagctggtcggcaacacgccctcacgcgagcaggttgaagaattgattctgtggcttggcgagcgggagatcgccgatgtcttcgcgaccgacaatcttgctggtaactaccccaccgccgcggcttatgcttccgttgcgagcggcattatcgcgatgcgggtttcggagttgcacggcagctggctcatctggtttcggccggaagtcataaaaacagttcgttggggtggtgatcctcacaagacggttcaggagtctggtcgtattcatcctcgtaagtcctttgaaatctggaaggaacaattgcgtaacacctcctttccttggtcagagcctgaacttgcagcagcgcgtgaacttcgtggtgcaatcatcggtattgtcctgcgtaagacagaggaaatggcagatctgacacgtgagttgcagcgtaccaataaagagctggaagctttttcctattccgtttctcatgatctgcgtgctccgttccgtcatattgtcgggtttgcacagttgttgcgtgagcgttccgatgccctggatgagaagtccctgcattatctacagatgatatcagaggcagcgctgggtgcaggcaggttggtggatgatctcctgaatttttcacagcttggtcgaacgcaactcaccctgaaacctgttgatatgcaaaaagttgtgtctgaagttcgtcgttccctgtcacacgcggtttccgatcgccagatcgagtggcgcatcggagcgttgccggtgatattcggggatccgaccctgctgcggcaggtctggtacaatctcattgaaaacgccatcaaatattcgtcccgggagccggtgtcgatcatcaccatttctgcggttgaaaccgaggatgacgtcacttattcggtcgaagacaatggtgtcggctttgacatggcctattacaacaaactttttggcgtgtttcagaggctacagcgggtggaggatttcgagggaactggcattggacttgcgctggtgcgacgaattgtcgaacgccatcacggtttggttggtgccgagggaactgtcggtgagggagccacgttctccttcacattgccggttacgaaagtggaagaagaaaaaattgcgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z