BBa_I712074
1
BBa_I712074
T7 promoter (strong promoter from T7 bacteriophage)
2007-10-21T11:00:00Z
2015-08-31T04:07:46Z
T7 bacteriophage
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
false
false
_130_
0
1856
9
In stock
false
true
Rok Gaber
BBa_K953006
1
BBa_K953006
Heme Oxygenase with T7 + Deinococcus radiodurans Bacteriophytochrome (E. coli Codon Optimised)
2012-09-24T11:00:00Z
2015-05-08T01:13:49Z
source
description
false
false
_1221_
0
14275
9
Not in stock
false
design
false
Daniel Winter
component2199086
1
BBa_K953001
component2199080
1
BBa_K953000
annotation2199086
1
BBa_K953001
range2199086
1
795
3090
annotation2199080
1
BBa_K953000
range2199080
1
1
794
BBa_K953001
1
BBa_K953001
Deinococcus radiodurans Bacteriophytochrome with RBS (E. coli Codon Optimised)
2012-09-22T11:00:00Z
2015-05-08T01:13:49Z
Deinococcus radiodurans
Deinococcus radiodurans bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light(620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time.
As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells.
false
false
_1221_
0
14275
9
In stock
true
Optimised for expression in E. coli
false
Daniel Winter
annotation2196378
1
Start Codon
range2196378
1
23
25
annotation2196379
1
Bacteriophytochrome
range2196379
1
26
2290
annotation2196380
1
Stop Codon
range2196380
1
2291
2296
annotation2196377
1
BBa_K953001
range2196377
1
1
2296
annotation2196376
1
RBS
range2196376
1
1
22
BBa_K953000
1
BBa_K953000
Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)
2012-09-19T11:00:00Z
2015-05-08T01:13:49Z
The part was codon optimsed (http://www.bioinformatics.org/sms2/rev_trans.html) from Synechocystis and assembled using Gibson Assembly. Select GC rich regions were removed.
E. coli codon optimised Synechocystis Heme Oxygenase with a Ribosome Binding Site (RBS) and T7 promoter (<html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_I719005">Part BBa1719005</a></html>, oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.
false
false
_1221_
0
14254
9
It's complicated
true
GC rich regions, which formed hairpin loops in synthesis, were removed. Restriction sites were removed to conform to biobrick standards.
false
Ryan Kenny
component2199289
1
BBa_I712074
component2199294
1
BBa_K953004
annotation2199289
1
BBa_I712074
range2199289
1
1
46
annotation2199294
1
BBa_K953004
range2199294
1
47
794
BBa_K953004
1
BBa_K953004
Hemeoxygenase with RBS (E. coli Codon Optimised)
2012-09-24T11:00:00Z
2015-05-08T01:13:49Z
Gibson assembly
E. coli codon optimised Synechocystis Heme Oxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.
false
false
_1221_
0
10455
9
Not in stock
false
Codon optimisation for E. coli, whilst staying within the GC constraints of gibson assembly.
false
David Dubow
annotation2199033
1
Stop Codon
range2199033
1
743
748
annotation2199030
1
RBS
range2199030
1
1
21
annotation2199031
1
Start Codon
range2199031
1
23
25
annotation2199032
1
Heme Oxygenase
range2199032
1
26
742
BBa_K953006_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcactttaagaaggagatatacataatgtctgttaatctggcgtctcaactgcgcgaaggtaccaaaaaatctcatagcatggcggaaaatgttggctttgtgaaatgctttctgaaaggtgttgttgaaaaaaattcttatcgcaaactggttggtaatctgtattttgtgtattctgcgatggaagaagaaatggcgaaatttaaagatcatccgattctgtctcatatttattttccggaactgaatcgcaaacaatctctggaacaagatcttcagttttattatggttctaattggcgccaggaagttaaaatttctgcggcgggtcaggcgtatgttgatcgcgttcgccaggttgcggcgacagcgccggaactgctggtggcgcatagctatacccgctatctgggcgatctgagcggcggccagattctgaaaaaaattgcgcagaacgcgatgaacctgcatgatggcggcaccgcgttttatgaatttgcggatattgatgatgaaaaagcgtttaaaaacacctatcgccaggcgatgaacgatctgccgattgatcaggcgaccgcggaacgcattgtggatgaagcgaacgatgcgtttgcgatgaacatgaaaatgtttaacgaactggaaggcaacctgattaaagcgattggcattatggtgtttaacagcctgacccgccgccgcagccagggcagcaccgaagtgggcctggcgaccagcgaaggctaataactttaagaaggagatatacataatgtctcgcgatccgctgccgttttttccgccgctgtatctgggtggtccggaaattaccacagaaaattgtgaacgcgaaccgattcatattccgggttctattcagccgcatggtgcgctgctgacagcggatggtcattctggtgaagttcttcagatgtctctgaatgcggcgacatttctgggtcaggaaccgacagttctgcgcggtcagacactggcggcgctgctgccggaacagtggccggcgcttcaggcggcgctgccgccgggctgtccggatgcgcttcagtatcgcgcgacactggattggccggcggcgggtcatctgtctctgacagttcatcgcgtgggtgaactgctgattctggaatttgaaccgaccgaagcgtgggattctacaggtcctcatgctttacgtaacgcaatgttcgcactggaaagcgcgccgaatctgcgtgcacttgcggaagtggcgacccagaccgtgcgtgaactgaccggttttgatcgtgtgatgctgtataaatttgcgccggatgcgaccggtgaagtgattgcggaagcgcgtcgtgaaggtctgcatgcgtttctgggtcatcgttttccggcgagcgatattccggcgcaggcgcgtgctctctatacccgtcatctgctgcgtctgaccgcggatacccgtgcggcggcggtgccgctggatccggtgctgaatccgcagaccaatgcgccgaccccgctgggtggtgcggtgctgcgtgcgaccagcccgatgcatatgcagtatctgcgtaatatgggtgtgggtagcagcctgagcgtgagcgtggtggtgggtggtcagctgtggggtctgattgcgtgtcatcatcagacaccttatgttcttcctccagatctgcgtacaacactggaatatctgggtcgtctgctgtctcttcaagttcaagttaaagaagcggcggatgttgcggcgtttcgtcaatctctgcgtgaacatcatgcgcgtgttgcgctggcggcggcgcattctctgtctccgcatgatacactgtctgatccggcgctggatctgctgggtctgatgcgtgcgggtggtctgattctgcgttttgaaggtcgttggcaaacactgggtgaagttcctcctgcgcctgctgttgatgcgctgctggcgtggctggaaacacaaccgggtgcgctggttcaaacagatgcgctgggtcagctgtggccggcgggtgctgatcttgctccttctgcggctggtcttctggcgatttctgttggtgaaggttggtctgaatgtctggtttggctgcgtccggaactgcgtctggaagtggcgtggggcggcgcgaccccggatcaggcgaaagatgatctgggcccgcgccatagctttgatacctatctggaagaaaaacgcggctatgcggaaccgtggcatccgggcgaaattgaagaagcgcaggatctgcgcgataccctgaccggcgcgctgggcgaacgcctgagcgtgattcgcgatctgaaccgcgcgctgacccagagcaacgcggaatggcgccagtatggctttgtgattagccatcatatgcaggaaccggtgcgcctgattagccagtttgcggaactgctgacccgccagccgcgcgcgcaggatggcagcccggatagcccgcagaccgaacgcattaccggctttctgttgcgcgaaaccagccgcctgcgttccctgacccaggatctgcatacctataccgcgctgctgagcgcgccgccgccggtgcgccgcccgaccccgctgggccgcgtggtggatgatgtgcttcaggatctggaaccgcgcattgcggatacaggtgcgtctattgaagttgcgccggaactgccggttattgcggcggatgcgggtctgctgcgcgatctgctgctgcatctgattggtaatgcgctgacatttggtggtccggaaccgcgcattgcggttcgcacagaacgccaaggtgcgggttggtctattgcggtttctgatcagggtgcgggtattgcgccggaatatcaggaacgcatttttctgctgtttcaacgcctgggtagcctggatgaagcgctgggtaatggtctgggtctgccgctgtgtcgcaaaattgcggaactgcatggtggtacactgacagttgaatctgcgccgggtgaaggtagcacatttcgctgttggctgccggatgcgggtccgctgccgggtgcggcggatgcgtgataataa
BBa_I712074_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgca
BBa_K953000_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcactttaagaaggagatatacataatgtctgttaatctggcgtctcaactgcgcgaaggtaccaaaaaatctcatagcatggcggaaaatgttggctttgtgaaatgctttctgaaaggtgttgttgaaaaaaattcttatcgcaaactggttggtaatctgtattttgtgtattctgcgatggaagaagaaatggcgaaatttaaagatcatccgattctgtctcatatttattttccggaactgaatcgcaaacaatctctggaacaagatcttcagttttattatggttctaattggcgccaggaagttaaaatttctgcggcgggtcaggcgtatgttgatcgcgttcgccaggttgcggcgacagcgccggaactgctggtggcgcatagctatacccgctatctgggcgatctgagcggcggccagattctgaaaaaaattgcgcagaacgcgatgaacctgcatgatggcggcaccgcgttttatgaatttgcggatattgatgatgaaaaagcgtttaaaaacacctatcgccaggcgatgaacgatctgccgattgatcaggcgaccgcggaacgcattgtggatgaagcgaacgatgcgtttgcgatgaacatgaaaatgtttaacgaactggaaggcaacctgattaaagcgattggcattatggtgtttaacagcctgacccgccgccgcagccagggcagcaccgaagtgggcctggcgaccagcgaaggctaataa
BBa_K953004_sequence
1
ctttaagaaggagatatacataatgtctgttaatctggcgtctcaactgcgcgaaggtaccaaaaaatctcatagcatggcggaaaatgttggctttgtgaaatgctttctgaaaggtgttgttgaaaaaaattcttatcgcaaactggttggtaatctgtattttgtgtattctgcgatggaagaagaaatggcgaaatttaaagatcatccgattctgtctcatatttattttccggaactgaatcgcaaacaatctctggaacaagatcttcagttttattatggttctaattggcgccaggaagttaaaatttctgcggcgggtcaggcgtatgttgatcgcgttcgccaggttgcggcgacagcgccggaactgctggtggcgcatagctatacccgctatctgggcgatctgagcggcggccagattctgaaaaaaattgcgcagaacgcgatgaacctgcatgatggcggcaccgcgttttatgaatttgcggatattgatgatgaaaaagcgtttaaaaacacctatcgccaggcgatgaacgatctgccgattgatcaggcgaccgcggaacgcattgtggatgaagcgaacgatgcgtttgcgatgaacatgaaaatgtttaacgaactggaaggcaacctgattaaagcgattggcattatggtgtttaacagcctgacccgccgccgcagccagggcagcaccgaagtgggcctggcgaccagcgaaggctaataa
BBa_K953001_sequence
1
ctttaagaaggagatatacataatgtctcgcgatccgctgccgttttttccgccgctgtatctgggtggtccggaaattaccacagaaaattgtgaacgcgaaccgattcatattccgggttctattcagccgcatggtgcgctgctgacagcggatggtcattctggtgaagttcttcagatgtctctgaatgcggcgacatttctgggtcaggaaccgacagttctgcgcggtcagacactggcggcgctgctgccggaacagtggccggcgcttcaggcggcgctgccgccgggctgtccggatgcgcttcagtatcgcgcgacactggattggccggcggcgggtcatctgtctctgacagttcatcgcgtgggtgaactgctgattctggaatttgaaccgaccgaagcgtgggattctacaggtcctcatgctttacgtaacgcaatgttcgcactggaaagcgcgccgaatctgcgtgcacttgcggaagtggcgacccagaccgtgcgtgaactgaccggttttgatcgtgtgatgctgtataaatttgcgccggatgcgaccggtgaagtgattgcggaagcgcgtcgtgaaggtctgcatgcgtttctgggtcatcgttttccggcgagcgatattccggcgcaggcgcgtgctctctatacccgtcatctgctgcgtctgaccgcggatacccgtgcggcggcggtgccgctggatccggtgctgaatccgcagaccaatgcgccgaccccgctgggtggtgcggtgctgcgtgcgaccagcccgatgcatatgcagtatctgcgtaatatgggtgtgggtagcagcctgagcgtgagcgtggtggtgggtggtcagctgtggggtctgattgcgtgtcatcatcagacaccttatgttcttcctccagatctgcgtacaacactggaatatctgggtcgtctgctgtctcttcaagttcaagttaaagaagcggcggatgttgcggcgtttcgtcaatctctgcgtgaacatcatgcgcgtgttgcgctggcggcggcgcattctctgtctccgcatgatacactgtctgatccggcgctggatctgctgggtctgatgcgtgcgggtggtctgattctgcgttttgaaggtcgttggcaaacactgggtgaagttcctcctgcgcctgctgttgatgcgctgctggcgtggctggaaacacaaccgggtgcgctggttcaaacagatgcgctgggtcagctgtggccggcgggtgctgatcttgctccttctgcggctggtcttctggcgatttctgttggtgaaggttggtctgaatgtctggtttggctgcgtccggaactgcgtctggaagtggcgtggggcggcgcgaccccggatcaggcgaaagatgatctgggcccgcgccatagctttgatacctatctggaagaaaaacgcggctatgcggaaccgtggcatccgggcgaaattgaagaagcgcaggatctgcgcgataccctgaccggcgcgctgggcgaacgcctgagcgtgattcgcgatctgaaccgcgcgctgacccagagcaacgcggaatggcgccagtatggctttgtgattagccatcatatgcaggaaccggtgcgcctgattagccagtttgcggaactgctgacccgccagccgcgcgcgcaggatggcagcccggatagcccgcagaccgaacgcattaccggctttctgttgcgcgaaaccagccgcctgcgttccctgacccaggatctgcatacctataccgcgctgctgagcgcgccgccgccggtgcgccgcccgaccccgctgggccgcgtggtggatgatgtgcttcaggatctggaaccgcgcattgcggatacaggtgcgtctattgaagttgcgccggaactgccggttattgcggcggatgcgggtctgctgcgcgatctgctgctgcatctgattggtaatgcgctgacatttggtggtccggaaccgcgcattgcggttcgcacagaacgccaaggtgcgggttggtctattgcggtttctgatcagggtgcgggtattgcgccggaatatcaggaacgcatttttctgctgtttcaacgcctgggtagcctggatgaagcgctgggtaatggtctgggtctgccgctgtgtcgcaaaattgcggaactgcatggtggtacactgacagttgaatctgcgccgggtgaaggtagcacatttcgctgttggctgccggatgcgggtccgctgccgggtgcggcggatgcgtgataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z