BBa_K953000 1 BBa_K953000 Heme Oxygenase with T7 & RBS (E. coli Codon Optimised) 2012-09-19T11:00:00Z 2015-05-08T01:13:49Z The part was codon optimsed (http://www.bioinformatics.org/sms2/rev_trans.html) from Synechocystis and assembled using Gibson Assembly. Select GC rich regions were removed. E. coli codon optimised Synechocystis Heme Oxygenase with a Ribosome Binding Site (RBS) and T7 promoter (<html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_I719005">Part BBa1719005</a></html>, oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration. false false _1221_ 0 14254 9 It's complicated true GC rich regions, which formed hairpin loops in synthesis, were removed. Restriction sites were removed to conform to biobrick standards. false Ryan Kenny component2199294 1 BBa_K953004 component2199289 1 BBa_I712074 annotation2199289 1 BBa_I712074 range2199289 1 1 46 annotation2199294 1 BBa_K953004 range2199294 1 47 794 BBa_K953004 1 BBa_K953004 Hemeoxygenase with RBS (E. coli Codon Optimised) 2012-09-24T11:00:00Z 2015-05-08T01:13:49Z Gibson assembly E. coli codon optimised Synechocystis Heme Oxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration. false false _1221_ 0 10455 9 Not in stock false Codon optimisation for E. coli, whilst staying within the GC constraints of gibson assembly. false David Dubow annotation2199031 1 Start Codon range2199031 1 23 25 annotation2199033 1 Stop Codon range2199033 1 743 748 annotation2199032 1 Heme Oxygenase range2199032 1 26 742 annotation2199030 1 RBS range2199030 1 1 21 BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_K953006 1 BBa_K953006 Heme Oxygenase with T7 + Deinococcus radiodurans Bacteriophytochrome (E. coli Codon Optimised) 2012-09-24T11:00:00Z 2015-05-08T01:13:49Z source description false false _1221_ 0 14275 9 Not in stock false design false Daniel Winter component2199086 1 BBa_K953001 component2199080 1 BBa_K953000 annotation2199080 1 BBa_K953000 range2199080 1 1 794 annotation2199086 1 BBa_K953001 range2199086 1 795 3090 BBa_K953001 1 BBa_K953001 Deinococcus radiodurans Bacteriophytochrome with RBS (E. coli Codon Optimised) 2012-09-22T11:00:00Z 2015-05-08T01:13:49Z Deinococcus radiodurans Deinococcus radiodurans bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light(620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells. false false _1221_ 0 14275 9 In stock true Optimised for expression in E. coli false Daniel Winter annotation2196379 1 Bacteriophytochrome range2196379 1 26 2290 annotation2196380 1 Stop Codon range2196380 1 2291 2296 annotation2196378 1 Start Codon range2196378 1 23 25 annotation2196377 1 BBa_K953001 range2196377 1 1 2296 annotation2196376 1 RBS range2196376 1 1 22 BBa_K953006_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcactttaagaaggagatatacataatgtctgttaatctggcgtctcaactgcgcgaaggtaccaaaaaatctcatagcatggcggaaaatgttggctttgtgaaatgctttctgaaaggtgttgttgaaaaaaattcttatcgcaaactggttggtaatctgtattttgtgtattctgcgatggaagaagaaatggcgaaatttaaagatcatccgattctgtctcatatttattttccggaactgaatcgcaaacaatctctggaacaagatcttcagttttattatggttctaattggcgccaggaagttaaaatttctgcggcgggtcaggcgtatgttgatcgcgttcgccaggttgcggcgacagcgccggaactgctggtggcgcatagctatacccgctatctgggcgatctgagcggcggccagattctgaaaaaaattgcgcagaacgcgatgaacctgcatgatggcggcaccgcgttttatgaatttgcggatattgatgatgaaaaagcgtttaaaaacacctatcgccaggcgatgaacgatctgccgattgatcaggcgaccgcggaacgcattgtggatgaagcgaacgatgcgtttgcgatgaacatgaaaatgtttaacgaactggaaggcaacctgattaaagcgattggcattatggtgtttaacagcctgacccgccgccgcagccagggcagcaccgaagtgggcctggcgaccagcgaaggctaataactttaagaaggagatatacataatgtctcgcgatccgctgccgttttttccgccgctgtatctgggtggtccggaaattaccacagaaaattgtgaacgcgaaccgattcatattccgggttctattcagccgcatggtgcgctgctgacagcggatggtcattctggtgaagttcttcagatgtctctgaatgcggcgacatttctgggtcaggaaccgacagttctgcgcggtcagacactggcggcgctgctgccggaacagtggccggcgcttcaggcggcgctgccgccgggctgtccggatgcgcttcagtatcgcgcgacactggattggccggcggcgggtcatctgtctctgacagttcatcgcgtgggtgaactgctgattctggaatttgaaccgaccgaagcgtgggattctacaggtcctcatgctttacgtaacgcaatgttcgcactggaaagcgcgccgaatctgcgtgcacttgcggaagtggcgacccagaccgtgcgtgaactgaccggttttgatcgtgtgatgctgtataaatttgcgccggatgcgaccggtgaagtgattgcggaagcgcgtcgtgaaggtctgcatgcgtttctgggtcatcgttttccggcgagcgatattccggcgcaggcgcgtgctctctatacccgtcatctgctgcgtctgaccgcggatacccgtgcggcggcggtgccgctggatccggtgctgaatccgcagaccaatgcgccgaccccgctgggtggtgcggtgctgcgtgcgaccagcccgatgcatatgcagtatctgcgtaatatgggtgtgggtagcagcctgagcgtgagcgtggtggtgggtggtcagctgtggggtctgattgcgtgtcatcatcagacaccttatgttcttcctccagatctgcgtacaacactggaatatctgggtcgtctgctgtctcttcaagttcaagttaaagaagcggcggatgttgcggcgtttcgtcaatctctgcgtgaacatcatgcgcgtgttgcgctggcggcggcgcattctctgtctccgcatgatacactgtctgatccggcgctggatctgctgggtctgatgcgtgcgggtggtctgattctgcgttttgaaggtcgttggcaaacactgggtgaagttcctcctgcgcctgctgttgatgcgctgctggcgtggctggaaacacaaccgggtgcgctggttcaaacagatgcgctgggtcagctgtggccggcgggtgctgatcttgctccttctgcggctggtcttctggcgatttctgttggtgaaggttggtctgaatgtctggtttggctgcgtccggaactgcgtctggaagtggcgtggggcggcgcgaccccggatcaggcgaaagatgatctgggcccgcgccatagctttgatacctatctggaagaaaaacgcggctatgcggaaccgtggcatccgggcgaaattgaagaagcgcaggatctgcgcgataccctgaccggcgcgctgggcgaacgcctgagcgtgattcgcgatctgaaccgcgcgctgacccagagcaacgcggaatggcgccagtatggctttgtgattagccatcatatgcaggaaccggtgcgcctgattagccagtttgcggaactgctgacccgccagccgcgcgcgcaggatggcagcccggatagcccgcagaccgaacgcattaccggctttctgttgcgcgaaaccagccgcctgcgttccctgacccaggatctgcatacctataccgcgctgctgagcgcgccgccgccggtgcgccgcccgaccccgctgggccgcgtggtggatgatgtgcttcaggatctggaaccgcgcattgcggatacaggtgcgtctattgaagttgcgccggaactgccggttattgcggcggatgcgggtctgctgcgcgatctgctgctgcatctgattggtaatgcgctgacatttggtggtccggaaccgcgcattgcggttcgcacagaacgccaaggtgcgggttggtctattgcggtttctgatcagggtgcgggtattgcgccggaatatcaggaacgcatttttctgctgtttcaacgcctgggtagcctggatgaagcgctgggtaatggtctgggtctgccgctgtgtcgcaaaattgcggaactgcatggtggtacactgacagttgaatctgcgccgggtgaaggtagcacatttcgctgttggctgccggatgcgggtccgctgccgggtgcggcggatgcgtgataataa BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_K953000_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcactttaagaaggagatatacataatgtctgttaatctggcgtctcaactgcgcgaaggtaccaaaaaatctcatagcatggcggaaaatgttggctttgtgaaatgctttctgaaaggtgttgttgaaaaaaattcttatcgcaaactggttggtaatctgtattttgtgtattctgcgatggaagaagaaatggcgaaatttaaagatcatccgattctgtctcatatttattttccggaactgaatcgcaaacaatctctggaacaagatcttcagttttattatggttctaattggcgccaggaagttaaaatttctgcggcgggtcaggcgtatgttgatcgcgttcgccaggttgcggcgacagcgccggaactgctggtggcgcatagctatacccgctatctgggcgatctgagcggcggccagattctgaaaaaaattgcgcagaacgcgatgaacctgcatgatggcggcaccgcgttttatgaatttgcggatattgatgatgaaaaagcgtttaaaaacacctatcgccaggcgatgaacgatctgccgattgatcaggcgaccgcggaacgcattgtggatgaagcgaacgatgcgtttgcgatgaacatgaaaatgtttaacgaactggaaggcaacctgattaaagcgattggcattatggtgtttaacagcctgacccgccgccgcagccagggcagcaccgaagtgggcctggcgaccagcgaaggctaataa BBa_K953004_sequence 1 ctttaagaaggagatatacataatgtctgttaatctggcgtctcaactgcgcgaaggtaccaaaaaatctcatagcatggcggaaaatgttggctttgtgaaatgctttctgaaaggtgttgttgaaaaaaattcttatcgcaaactggttggtaatctgtattttgtgtattctgcgatggaagaagaaatggcgaaatttaaagatcatccgattctgtctcatatttattttccggaactgaatcgcaaacaatctctggaacaagatcttcagttttattatggttctaattggcgccaggaagttaaaatttctgcggcgggtcaggcgtatgttgatcgcgttcgccaggttgcggcgacagcgccggaactgctggtggcgcatagctatacccgctatctgggcgatctgagcggcggccagattctgaaaaaaattgcgcagaacgcgatgaacctgcatgatggcggcaccgcgttttatgaatttgcggatattgatgatgaaaaagcgtttaaaaacacctatcgccaggcgatgaacgatctgccgattgatcaggcgaccgcggaacgcattgtggatgaagcgaacgatgcgtttgcgatgaacatgaaaatgtttaacgaactggaaggcaacctgattaaagcgattggcattatggtgtttaacagcctgacccgccgccgcagccagggcagcaccgaagtgggcctggcgaccagcgaaggctaataa BBa_K953001_sequence 1 ctttaagaaggagatatacataatgtctcgcgatccgctgccgttttttccgccgctgtatctgggtggtccggaaattaccacagaaaattgtgaacgcgaaccgattcatattccgggttctattcagccgcatggtgcgctgctgacagcggatggtcattctggtgaagttcttcagatgtctctgaatgcggcgacatttctgggtcaggaaccgacagttctgcgcggtcagacactggcggcgctgctgccggaacagtggccggcgcttcaggcggcgctgccgccgggctgtccggatgcgcttcagtatcgcgcgacactggattggccggcggcgggtcatctgtctctgacagttcatcgcgtgggtgaactgctgattctggaatttgaaccgaccgaagcgtgggattctacaggtcctcatgctttacgtaacgcaatgttcgcactggaaagcgcgccgaatctgcgtgcacttgcggaagtggcgacccagaccgtgcgtgaactgaccggttttgatcgtgtgatgctgtataaatttgcgccggatgcgaccggtgaagtgattgcggaagcgcgtcgtgaaggtctgcatgcgtttctgggtcatcgttttccggcgagcgatattccggcgcaggcgcgtgctctctatacccgtcatctgctgcgtctgaccgcggatacccgtgcggcggcggtgccgctggatccggtgctgaatccgcagaccaatgcgccgaccccgctgggtggtgcggtgctgcgtgcgaccagcccgatgcatatgcagtatctgcgtaatatgggtgtgggtagcagcctgagcgtgagcgtggtggtgggtggtcagctgtggggtctgattgcgtgtcatcatcagacaccttatgttcttcctccagatctgcgtacaacactggaatatctgggtcgtctgctgtctcttcaagttcaagttaaagaagcggcggatgttgcggcgtttcgtcaatctctgcgtgaacatcatgcgcgtgttgcgctggcggcggcgcattctctgtctccgcatgatacactgtctgatccggcgctggatctgctgggtctgatgcgtgcgggtggtctgattctgcgttttgaaggtcgttggcaaacactgggtgaagttcctcctgcgcctgctgttgatgcgctgctggcgtggctggaaacacaaccgggtgcgctggttcaaacagatgcgctgggtcagctgtggccggcgggtgctgatcttgctccttctgcggctggtcttctggcgatttctgttggtgaaggttggtctgaatgtctggtttggctgcgtccggaactgcgtctggaagtggcgtggggcggcgcgaccccggatcaggcgaaagatgatctgggcccgcgccatagctttgatacctatctggaagaaaaacgcggctatgcggaaccgtggcatccgggcgaaattgaagaagcgcaggatctgcgcgataccctgaccggcgcgctgggcgaacgcctgagcgtgattcgcgatctgaaccgcgcgctgacccagagcaacgcggaatggcgccagtatggctttgtgattagccatcatatgcaggaaccggtgcgcctgattagccagtttgcggaactgctgacccgccagccgcgcgcgcaggatggcagcccggatagcccgcagaccgaacgcattaccggctttctgttgcgcgaaaccagccgcctgcgttccctgacccaggatctgcatacctataccgcgctgctgagcgcgccgccgccggtgcgccgcccgaccccgctgggccgcgtggtggatgatgtgcttcaggatctggaaccgcgcattgcggatacaggtgcgtctattgaagttgcgccggaactgccggttattgcggcggatgcgggtctgctgcgcgatctgctgctgcatctgattggtaatgcgctgacatttggtggtccggaaccgcgcattgcggttcgcacagaacgccaaggtgcgggttggtctattgcggtttctgatcagggtgcgggtattgcgccggaatatcaggaacgcatttttctgctgtttcaacgcctgggtagcctggatgaagcgctgggtaatggtctgggtctgccgctgtgtcgcaaaattgcggaactgcatggtggtacactgacagttgaatctgcgccgggtgaaggtagcacatttcgctgttggctgccggatgcgggtccgctgccgggtgcggcggatgcgtgataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z