BBa_K953004 1 BBa_K953004 Hemeoxygenase with RBS (E. coli Codon Optimised) 2012-09-24T11:00:00Z 2015-05-08T01:13:49Z Gibson assembly E. coli codon optimised Synechocystis Heme Oxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration. false false _1221_ 0 10455 9 Not in stock false Codon optimisation for E. coli, whilst staying within the GC constraints of gibson assembly. false David Dubow annotation2199031 1 Start Codon range2199031 1 23 25 annotation2199030 1 RBS range2199030 1 1 21 annotation2199032 1 Heme Oxygenase range2199032 1 26 742 annotation2199033 1 Stop Codon range2199033 1 743 748 BBa_K953002 1 BBa_K953002 Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised) 2012-09-23T11:00:00Z 2015-05-08T01:13:49Z Agrobacterium tumefaciens Released HQ 2013 Agrobacterium tumefaciens bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light(620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells. false false _1221_ 0 14275 9 In stock true Optimised for expression in E. coli false Daniel Winter annotation2196369 1 Start Codon range2196369 1 23 25 annotation2196370 1 Bacteriophytochrome range2196370 1 26 2230 annotation2196372 1 BBa_K953002 range2196372 1 1 2236 annotation2196371 1 Stop range2196371 1 2231 2236 annotation2196368 1 RBS range2196368 1 1 22 BBa_K953007 1 BBa_K953007 Agrobacterium tumefaciens Bacteriophytochrome with T7 + Heme Oxygenase (E. coli Codon Optimised) 2012-09-24T11:00:00Z 2015-05-08T01:13:49Z source description false false _1221_ 0 14275 9 Not in stock false design false Daniel Winter component2199386 1 BBa_K953003 component2199391 1 BBa_K953004 annotation2199391 1 BBa_K953004 range2199391 1 2283 3030 annotation2199386 1 BBa_K953003 range2199386 1 1 2282 BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_K953003 1 BBa_K953003 Agrobacterium tumefaciens Bacteriophytochrome with T7 & RBS (E. coli Codon Optimised) 2012-09-24T11:00:00Z 2015-05-08T01:13:49Z Gibson Assembly Agrobacterium tumefaciens bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light (620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953004) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells. This part contains a T7 Promotor. false false _1221_ 0 10455 9 Not in stock false Codon optimisation for E. coli, whilst staying within the GC constraints of gibson assembly. false David Dubow component2199064 1 BBa_K953002 component2199062 1 BBa_I712074 annotation2199064 1 BBa_K953002 range2199064 1 47 2282 annotation2199062 1 BBa_I712074 range2199062 1 1 46 BBa_K953002_sequence 1 ctttaagaaggagatatacataatgagttcacatacgccgaaactggatagttgcggcgcagagcccatccacattcccggtgctattcaggaacatggagcccttctggttctgtccgcgcgggaattttccgtggtgcaggcgagcgataatctcgccaactatattggtgtggacctgccaatcggggccgttgccaccgaagcaaacttgccgttcatctccgtactgtccgcctggtattccggcgaggagagcaatttccgatatgcctgggccgaaaagaaactcgatgtttcggcgcatcgatccgggaccttggttattcttgaggtggaaaaggcaggagtgggcgaatccgctgaaaagctgatgggcgaactcaccagtctggcgaaatatctgaacagtgcgccgtctctggaggacgcgctttttcggacagcgcaactggtgtcttctatcagcggtcatgaccgtacactgatctatgatttcggactggactggtctggtcatgttgtggcggaagcgggatctggtgctttgccgtcttatctcggtcttcgctttcctgcgggtgacatccctccgcaagcgcggcaactctatacaatcaatcgcctgcggatgattcctgatgttgattataagcctgttccgatcagaccggaggttaatgcggaaacaggagcggttctcgatatgagcttttctcaacttcgctctgtatctccggttcatctcgaatatatgcgcaatatgggtactgccgcttctatgtctgttagcatcgttgttaatggtgctctctggggcttgattgcctgtcaccacgcgacacctcattctgtatcattagcagttcgtgaagcctgtgattttgcagcgcagctcctgtcgatgcggatcgcgatggagcagagttcccaggatgcatcccgccgggtggagcttggccacattcaggcgcggctcttgaaaggcatggccgccgccgaaaaatgggttgatggcctgctgggaggcgaaggtgaaagagaagacctgctgaaacaggtgggcgcggatggcgcagcacttgtcctgggcgacgactatgagctggtcggcaacacgccctcacgcgagcaggttgaagaattgattctgtggcttggcgagcgggagatcgccgatgtcttcgcgaccgacaatcttgctggtaactaccccaccgccgcggcttatgcttccgttgcgagcggcattatcgcgatgcgggtttcggagttgcacggcagctggctcatctggtttcggccggaagtcataaaaacagttcgttggggtggtgatcctcacaagacggttcaggagtctggtcgtattcatcctcgtaagtcctttgaaatctggaaggaacaattgcgtaacacctcctttccttggtcagagcctgaacttgcagcagcgcgtgaacttcgtggtgcaatcatcggtattgtcctgcgtaagacagaggaaatggcagatctgacacgtgagttgcagcgtaccaataaagagctggaagctttttcctattccgtttctcatgatctgcgtgctccgttccgtcatattgtcgggtttgcacagttgttgcgtgagcgttccgatgccctggatgagaagtccctgcattatctacagatgatatcagaggcagcgctgggtgcaggcaggttggtggatgatctcctgaatttttcacagcttggtcgaacgcaactcaccctgaaacctgttgatatgcaaaaagttgtgtctgaagttcgtcgttccctgtcacacgcggtttccgatcgccagatcgagtggcgcatcggagcgttgccggtgatattcggggatccgaccctgctgcggcaggtctggtacaatctcattgaaaacgccatcaaatattcgtcccgggagccggtgtcgatcatcaccatttctgcggttgaaaccgaggatgacgtcacttattcggtcgaagacaatggtgtcggctttgacatggcctattacaacaaactttttggcgtgtttcagaggctacagcgggtggaggatttcgagggaactggcattggacttgcgctggtgcgacgaattgtcgaacgccatcacggtttggttggtgccgagggaactgtcggtgagggagccacgttctccttcacattgccggttacgaaagtggaagaagaaaaaattgcgtaataa BBa_K953003_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcactttaagaaggagatatacataatgagttcacatacgccgaaactggatagttgcggcgcagagcccatccacattcccggtgctattcaggaacatggagcccttctggttctgtccgcgcgggaattttccgtggtgcaggcgagcgataatctcgccaactatattggtgtggacctgccaatcggggccgttgccaccgaagcaaacttgccgttcatctccgtactgtccgcctggtattccggcgaggagagcaatttccgatatgcctgggccgaaaagaaactcgatgtttcggcgcatcgatccgggaccttggttattcttgaggtggaaaaggcaggagtgggcgaatccgctgaaaagctgatgggcgaactcaccagtctggcgaaatatctgaacagtgcgccgtctctggaggacgcgctttttcggacagcgcaactggtgtcttctatcagcggtcatgaccgtacactgatctatgatttcggactggactggtctggtcatgttgtggcggaagcgggatctggtgctttgccgtcttatctcggtcttcgctttcctgcgggtgacatccctccgcaagcgcggcaactctatacaatcaatcgcctgcggatgattcctgatgttgattataagcctgttccgatcagaccggaggttaatgcggaaacaggagcggttctcgatatgagcttttctcaacttcgctctgtatctccggttcatctcgaatatatgcgcaatatgggtactgccgcttctatgtctgttagcatcgttgttaatggtgctctctggggcttgattgcctgtcaccacgcgacacctcattctgtatcattagcagttcgtgaagcctgtgattttgcagcgcagctcctgtcgatgcggatcgcgatggagcagagttcccaggatgcatcccgccgggtggagcttggccacattcaggcgcggctcttgaaaggcatggccgccgccgaaaaatgggttgatggcctgctgggaggcgaaggtgaaagagaagacctgctgaaacaggtgggcgcggatggcgcagcacttgtcctgggcgacgactatgagctggtcggcaacacgccctcacgcgagcaggttgaagaattgattctgtggcttggcgagcgggagatcgccgatgtcttcgcgaccgacaatcttgctggtaactaccccaccgccgcggcttatgcttccgttgcgagcggcattatcgcgatgcgggtttcggagttgcacggcagctggctcatctggtttcggccggaagtcataaaaacagttcgttggggtggtgatcctcacaagacggttcaggagtctggtcgtattcatcctcgtaagtcctttgaaatctggaaggaacaattgcgtaacacctcctttccttggtcagagcctgaacttgcagcagcgcgtgaacttcgtggtgcaatcatcggtattgtcctgcgtaagacagaggaaatggcagatctgacacgtgagttgcagcgtaccaataaagagctggaagctttttcctattccgtttctcatgatctgcgtgctccgttccgtcatattgtcgggtttgcacagttgttgcgtgagcgttccgatgccctggatgagaagtccctgcattatctacagatgatatcagaggcagcgctgggtgcaggcaggttggtggatgatctcctgaatttttcacagcttggtcgaacgcaactcaccctgaaacctgttgatatgcaaaaagttgtgtctgaagttcgtcgttccctgtcacacgcggtttccgatcgccagatcgagtggcgcatcggagcgttgccggtgatattcggggatccgaccctgctgcggcaggtctggtacaatctcattgaaaacgccatcaaatattcgtcccgggagccggtgtcgatcatcaccatttctgcggttgaaaccgaggatgacgtcacttattcggtcgaagacaatggtgtcggctttgacatggcctattacaacaaactttttggcgtgtttcagaggctacagcgggtggaggatttcgagggaactggcattggacttgcgctggtgcgacgaattgtcgaacgccatcacggtttggttggtgccgagggaactgtcggtgagggagccacgttctccttcacattgccggttacgaaagtggaagaagaaaaaattgcgtaataa BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_K953004_sequence 1 ctttaagaaggagatatacataatgtctgttaatctggcgtctcaactgcgcgaaggtaccaaaaaatctcatagcatggcggaaaatgttggctttgtgaaatgctttctgaaaggtgttgttgaaaaaaattcttatcgcaaactggttggtaatctgtattttgtgtattctgcgatggaagaagaaatggcgaaatttaaagatcatccgattctgtctcatatttattttccggaactgaatcgcaaacaatctctggaacaagatcttcagttttattatggttctaattggcgccaggaagttaaaatttctgcggcgggtcaggcgtatgttgatcgcgttcgccaggttgcggcgacagcgccggaactgctggtggcgcatagctatacccgctatctgggcgatctgagcggcggccagattctgaaaaaaattgcgcagaacgcgatgaacctgcatgatggcggcaccgcgttttatgaatttgcggatattgatgatgaaaaagcgtttaaaaacacctatcgccaggcgatgaacgatctgccgattgatcaggcgaccgcggaacgcattgtggatgaagcgaacgatgcgtttgcgatgaacatgaaaatgtttaacgaactggaaggcaacctgattaaagcgattggcattatggtgtttaacagcctgacccgccgccgcagccagggcagcaccgaagtgggcctggcgaccagcgaaggctaataa BBa_K953007_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcactttaagaaggagatatacataatgagttcacatacgccgaaactggatagttgcggcgcagagcccatccacattcccggtgctattcaggaacatggagcccttctggttctgtccgcgcgggaattttccgtggtgcaggcgagcgataatctcgccaactatattggtgtggacctgccaatcggggccgttgccaccgaagcaaacttgccgttcatctccgtactgtccgcctggtattccggcgaggagagcaatttccgatatgcctgggccgaaaagaaactcgatgtttcggcgcatcgatccgggaccttggttattcttgaggtggaaaaggcaggagtgggcgaatccgctgaaaagctgatgggcgaactcaccagtctggcgaaatatctgaacagtgcgccgtctctggaggacgcgctttttcggacagcgcaactggtgtcttctatcagcggtcatgaccgtacactgatctatgatttcggactggactggtctggtcatgttgtggcggaagcgggatctggtgctttgccgtcttatctcggtcttcgctttcctgcgggtgacatccctccgcaagcgcggcaactctatacaatcaatcgcctgcggatgattcctgatgttgattataagcctgttccgatcagaccggaggttaatgcggaaacaggagcggttctcgatatgagcttttctcaacttcgctctgtatctccggttcatctcgaatatatgcgcaatatgggtactgccgcttctatgtctgttagcatcgttgttaatggtgctctctggggcttgattgcctgtcaccacgcgacacctcattctgtatcattagcagttcgtgaagcctgtgattttgcagcgcagctcctgtcgatgcggatcgcgatggagcagagttcccaggatgcatcccgccgggtggagcttggccacattcaggcgcggctcttgaaaggcatggccgccgccgaaaaatgggttgatggcctgctgggaggcgaaggtgaaagagaagacctgctgaaacaggtgggcgcggatggcgcagcacttgtcctgggcgacgactatgagctggtcggcaacacgccctcacgcgagcaggttgaagaattgattctgtggcttggcgagcgggagatcgccgatgtcttcgcgaccgacaatcttgctggtaactaccccaccgccgcggcttatgcttccgttgcgagcggcattatcgcgatgcgggtttcggagttgcacggcagctggctcatctggtttcggccggaagtcataaaaacagttcgttggggtggtgatcctcacaagacggttcaggagtctggtcgtattcatcctcgtaagtcctttgaaatctggaaggaacaattgcgtaacacctcctttccttggtcagagcctgaacttgcagcagcgcgtgaacttcgtggtgcaatcatcggtattgtcctgcgtaagacagaggaaatggcagatctgacacgtgagttgcagcgtaccaataaagagctggaagctttttcctattccgtttctcatgatctgcgtgctccgttccgtcatattgtcgggtttgcacagttgttgcgtgagcgttccgatgccctggatgagaagtccctgcattatctacagatgatatcagaggcagcgctgggtgcaggcaggttggtggatgatctcctgaatttttcacagcttggtcgaacgcaactcaccctgaaacctgttgatatgcaaaaagttgtgtctgaagttcgtcgttccctgtcacacgcggtttccgatcgccagatcgagtggcgcatcggagcgttgccggtgatattcggggatccgaccctgctgcggcaggtctggtacaatctcattgaaaacgccatcaaatattcgtcccgggagccggtgtcgatcatcaccatttctgcggttgaaaccgaggatgacgtcacttattcggtcgaagacaatggtgtcggctttgacatggcctattacaacaaactttttggcgtgtttcagaggctacagcgggtggaggatttcgagggaactggcattggacttgcgctggtgcgacgaattgtcgaacgccatcacggtttggttggtgccgagggaactgtcggtgagggagccacgttctccttcacattgccggttacgaaagtggaagaagaaaaaattgcgtaataactttaagaaggagatatacataatgtctgttaatctggcgtctcaactgcgcgaaggtaccaaaaaatctcatagcatggcggaaaatgttggctttgtgaaatgctttctgaaaggtgttgttgaaaaaaattcttatcgcaaactggttggtaatctgtattttgtgtattctgcgatggaagaagaaatggcgaaatttaaagatcatccgattctgtctcatatttattttccggaactgaatcgcaaacaatctctggaacaagatcttcagttttattatggttctaattggcgccaggaagttaaaatttctgcggcgggtcaggcgtatgttgatcgcgttcgccaggttgcggcgacagcgccggaactgctggtggcgcatagctatacccgctatctgggcgatctgagcggcggccagattctgaaaaaaattgcgcagaacgcgatgaacctgcatgatggcggcaccgcgttttatgaatttgcggatattgatgatgaaaaagcgtttaaaaacacctatcgccaggcgatgaacgatctgccgattgatcaggcgaccgcggaacgcattgtggatgaagcgaacgatgcgtttgcgatgaacatgaaaatgtttaacgaactggaaggcaacctgattaaagcgattggcattatggtgtttaacagcctgacccgccgccgcagccagggcagcaccgaagtgggcctggcgaccagcgaaggctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z