LOCUS       BBa_K957002             3207 bp    DNA     linear       28-Jun-2024
DEFINITION  Salicylate inducible mtrB with cut sites flanking RBS
ACCESSION   BBa_K957002
VERSION     BBa_K957002.1  
FEATURES             Location/Qualifiers
     CDS             1..903
                     /label=nahR
     misc_feature    1..1052
                     /label=BBa_K228004
     promoter        904..993
                     /label=Pr promoter
     promoter        982..1050
                     /label=pSal
     misc_feature    1079..3172
                     /label=BBa_K098989
     CDS             1079..3172
                     /label=mtrB
     misc_feature    3143..3143
                     /label=silent mutation, original base G
     misc_feature    3202..3207
                     /label=KpnI cut site
     misc_feature    1056..1063
                     /label=AscI cut site
     misc_feature    1073..1078
                     /label=BamHI cut site
     RBS             1066..1071
                     /label=mtrB rbs
ORIGIN
        1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg
       61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa
      121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag
      181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat
      241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc
      301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt
      361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa
      421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa
      481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg
      541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc
      601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt
      661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg
      721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt
      781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg
      841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc
      901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga
      961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca
     1021 agtcatcaat aaagccatca cgagtaccat agtacggcgc gcctaaggag acggatccat
     1081 gaaatttaaa ctcaatttga tcactctagc gttattagcc aacacaggct tggccgtcgc
     1141 tgctgatggt tatggtctag cgaatgccaa tactgaaaaa gtgaaattat ccgcatggag
     1201 ctgtaaaggc tgcgtcgttg aaacgggcac atcaggcact gtgggtgtcg gtgtcggtta
     1261 taacagcgaa gaggatattc gctctgccaa tgcctttggt acatccaatg aagtggcggg
     1321 taaatttgat gccgatttaa actttaaagg tgaaaagggt tatcgtgcca gtgttgatgc
     1381 ttatcaactc ggtatggatg gcggtcgctt agatgtcaat gcgggcaaac aaggccagta
     1441 caacgtcaat gtgaactatc gccaaattgc tacctacgac agcaatagcg ccctatcgcc
     1501 ctacgcgggt attggtggca ataacctcac gttaccggat aactggataa cagcaggttc
     1561 aagcaaccaa atgccactct tgatggacag cctcaatgcc ctcgaactct cacttaaacg
     1621 tgagcgcacg gggttgggat ttgaatatca aggtgaatcc ctgtggagca cctatgttaa
     1681 ctacatgcgt gaagagaaaa ccggcttaaa acaagcctct ggtagcttct tcaaccaatc
     1741 gatgatgtta gcagagccgg tggattacac cactgacacc attgaagcgg gtgtcaaact
     1801 caagggtgat cgttggttta ccgcactcag ttacaatggg tcaatattca aaaacgaata
     1861 caaccaattg gactttgaaa atgcttttaa ccccaccttt ggtgctcaaa cccaaggtac
     1921 gatggcactc gatccggata accagtcaca caccgtgtcg ctgatgggac agtacaacga
     1981 tggcagcaac gcactgtcgg gtcgtattct gaccggacaa atgagccaag atcaggcgtt
     2041 agtgacggat aactaccgtt atgctaatca gctcaatacc gatgccgtcg atgccaaagt
     2101 cgatctactg ggtatgaacc tgaaagtcgt tagcaaagtg agcaatgatc ttcgcttaac
     2161 aggtagttac gattattacg accgtgacaa taatacccaa gtagaagaat ggactcagat
     2221 cagcatcaac aatgtcaacg gtaaggtggc ttataacacc ccttacgata atcgtacgca
     2281 acgctttaaa gttgccgcag attatcgcat tacccgcgat atcaaactcg atggtggtta
     2341 tgacttcaaa cgtgaccaac gtgattatca agaccgtgaa accacggatg aaaataccgt
     2401 ttgggcccgt ttacgtgtaa acagcttcga tacttgggac atgtgggtaa aaggcagtta
     2461 cggtaaccgt gacggctcac aataccaagc gtctgaatgg acctcttctg aaaccaacag
     2521 cctgttacgt aagtacaatc tggctgaccg tgacagaact caagtcgaag cacggatcac
     2581 ccattcgcca ttagaaagcc tgactatcga tgttggtgcc cgttacgcgt tagatgatta
     2641 taccgatact gtgattggat taactgagtc aaaagacacc agttatgatg ccaacatcag
     2701 ttatatgatc accgctgact tactggcaac cgccttctac aattaccaaa ccattgagtc
     2761 tgaacaggcg ggtagcagca attacagcac cccaacgtgg acaggcttta tagaagatca
     2821 ggtagatgtg gtcggtgcag gtatcagcta caacaatctg ctggagaaca agttacgcct
     2881 aggactggac tacacctatt ccaactccga cagtaacact caagtcagac aaggtatcac
     2941 tggcgactat ggtgattatt ttgccaaagt gcataacatt aacttatacg ctcaatatca
     3001 agccaccgag aaactcgcgc tgcgcttcga ttacaaaatt gagaactata aggacaatga
     3061 cgccgcaaat gatatcgccg ttgatggcat ttggaacgtc gtaggttttg gtagtaacag
     3121 ccatgactac accgcacaaa tgttgatgct gagcatgagt tacaaactct aatactagag
     3181 tcacactggc tcaccttcta tggtacc
//