BBa_K957003 1 BBa_K957003 Salicylate inducible mtrB 2012-09-24T11:00:00Z 2015-05-08T01:13:49Z mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source. The salicylate sensitive region was isolated via PCR from a plasmid containing BBa_K228227, designed by PKU_Beijing 2009. The purpose of this part is to upregulate mtrB expression in response to salicylate. When expressed in a Shewanella strain lacking mtrB on the chromosome, this composite part will function as a component of a biosensor. Specifically, when such a complemented strain is inoculated in a microbial electrochemical system, current output will increase in response to salicylate. If coupled to the expression of a BioBrick part that degrades naphthalene to salicylate, this part will function as part of a naphthalene biosensor. false false _1228_ 0 13504 9 It's complicated true Constructed using non-BioBrick AscI cutsite of BBa_K957001. false Dylan Patrick Webster annotation2198933 1 Pr range2198933 1 904 993 annotation2198937 1 mtrB range2198937 1 1088 3181 annotation2198936 1 AscI cut site range2198936 1 1054 1061 annotation2198934 1 Psal range2198934 1 982 1050 annotation2198931 1 K228004 range2198931 1 1 1052 annotation2198932 1 nahR range2198932 1 1 903 annotation2198938 1 K098989 range2198938 1 1088 3181 BBa_K957003_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagcggcgcgcctactagagaaagaggagaaatactagatgaaatttaaactcaatttgatcactctagcgttattagccaacacaggcttggccgtcgctgctgatggttatggtctagcgaatgccaatactgaaaaagtgaaattatccgcatggagctgtaaaggctgcgtcgttgaaacgggcacatcaggcactgtgggtgtcggtgtcggttataacagcgaagaggatattcgctctgccaatgcctttggtacatccaatgaagtggcgggtaaatttgatgccgatttaaactttaaaggtgaaaagggttatcgtgccagtgttgatgcttatcaactcggtatggatggcggtcgcttagatgtcaatgcgggcaaacaaggccagtacaacgtcaatgtgaactatcgccaaattgctacctacgacagcaatagcgccctatcgccctacgcgggtattggtggcaataacctcacgttaccggataactggataacagcaggttcaagcaaccaaatgccactcttgatggacagcctcaatgccctcgaactctcacttaaacgtgagcgcacggggttgggatttgaatatcaaggtgaatccctgtggagcacctatgttaactacatgcgtgaagagaaaaccggcttaaaacaagcctctggtagcttcttcaaccaatcgatgatgttagcagagccggtggattacaccactgacaccattgaagcgggtgtcaaactcaagggtgatcgttggtttaccgcactcagttacaatgggtcaatattcaaaaacgaatacaaccaattggactttgaaaatgcttttaaccccacctttggtgctcaaacccaaggtacgatggcactcgatccggataaccagtcacacaccgtgtcgctgatgggacagtacaacgatggcagcaacgcactgtcgggtcgtattctgaccggacaaatgagccaagatcaggcgttagtgacggataactaccgttatgctaatcagctcaataccgatgccgtcgatgccaaagtcgatctactgggtatgaacctgaaagtcgttagcaaagtgagcaatgatcttcgcttaacaggtagttacgattattacgaccgtgacaataatacccaagtagaagaatggactcagatcagcatcaacaatgtcaacggtaaggtggcttataacaccccttacgataatcgtacgcaacgctttaaagttgccgcagattatcgcattacccgcgatatcaaactcgatggtggttatgacttcaaacgtgaccaacgtgattatcaagaccgtgaaaccacggatgaaaataccgtttgggcccgtttacgtgtaaacagcttcgatacttgggacatgtgggtaaaaggcagttacggtaaccgtgacggctcacaataccaagcgtctgaatggacctcttctgaaaccaacagcctgttacgtaagtacaatctggctgaccgtgacagaactcaagtcgaagcacggatcacccattcgccattagaaagcctgactatcgatgttggtgcccgttacgcgttagatgattataccgatactgtgattggattaactgagtcaaaagacaccagttatgatgccaacatcagttatatgatcaccgctgacttactggcaaccgccttctacaattaccaaaccattgagtctgaacaggcgggtagcagcaattacagcaccccaacgtggacaggctttatagaagatcaggtagatgtggtcggtgcaggtatcagctacaacaatctgctggagaacaagttacgcctaggactggactacacctattccaactccgacagtaacactcaagtcagacaaggtatcactggcgactatggtgattattttgccaaagtgcataacattaacttatacgctcaatatcaagccaccgagaaactcgcgctgcgcttcgattacaaaattgagaactataaggacaatgacgccgcaaatgatatcgccgttgatggcatttggaacgtcgtaggttttggtagtaacagccatgactacaccgcacaaatgctgatgctgagcatgagttacaaactctaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z