BBa_K957004 1 BBa_K957004 nah Operon for degradation of naphthalene to salicylate 2012-09-24T11:00:00Z 2015-05-08T01:13:49Z From NAH7 plasmid of Pseudomonas putida G7 (provided by Dr. Gene Madsen, Cornell University Department of Microbiology). One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene. false false _1228_ 0 13504 9 It's complicated true Three internal BioBrick cut sites (one NotI, two PstI) remain in this version of the part. However, the utility of the part is not restricted, since digestion with EcoRI and SpeI facilitates standard molecular cloning. false Dylan Patrick Webster annotation2199002 1 nahAa range2199002 1 13 999 annotation2199011 1 nahD range2199011 1 8795 9406 annotation2199006 1 nahB range2199006 1 3546 4325 annotation2199014 1 NotI cut site range2199014 1 8165 8172 annotation2199005 1 nahAd range2199005 1 2892 3476 annotation2199010 1 nahE range2199010 1 7535 8530 annotation2199012 1 PstI cut site range2199012 1 1867 1872 annotation2199013 1 PstI cut site range2199013 1 5862 5867 annotation2199003 1 nahAb range2199003 1 1143 1466 annotation2199009 1 nahQ range2199009 1 6854 7486 annotation2199007 1 nahF range2199007 1 4371 5822 annotation2199008 1 nahC range2199008 1 5849 6757 annotation2199004 1 nahAc range2199004 1 1528 2877 BBa_K957004_sequence 1 taagccggatttatggaacttctcatacagccaaacaatcgcctcattagctttagtcccggcgccaaccttctggaagtgcttcgcgaaaacggtgtcgctatttcctacagttgtatgtctgggcgttgcggaacctgccgctgtcgggttacagatggcagtgtaattgattcgggggcgggaagcgggttaccaaacctcgtggacgagcattatgtgctcgcctgtcagtcagtacttactcacaattgcgcgatcgaaatcccagaaaccgacgaaatcgtcacccacccggcgagaatcatcaagggcactgtggtcgccgtcgagtcgcccactcacgatatccgtcgcctacgcgtacgcctcgctaagcccttcgagttctcacccggacagtacgcgacattgcagttcagtcctgagcatgcgcgtccgtattcaatggcaggtctgccagatgaccaagaaatggagttccacatacgcaaggtgccgggtgggcgcgtaacggagtatgttttcgagcacgtccgcgaaggtacaagcatcaagttgagcgggccacttggtacggcttatttgcgtcagaaccacaccgggccgatgctctgtgtgggcggtgggaccggactagcaccggtgctgtcgattgttcgcggcgcgctgaagttgggtatgacaaaccccatcctcctttatttcggagtgcgcagtcagcaagacctctacgacgcagagcgattgcacaaactcgccgctgatcaccctcaactgaccgtacacacggtaatcgcaatgggcccgattaatgagagtcagcgagccggtctagttaccgatgtgatcgaaaaagacatcatttcgctggctgggtggagggcctacctgtgcggcgcaccagcgatggttgaagcgctttgcaccgttaccaagcatcttggaatatcacccgaacatatttatgccgatgccttctatcccggtggaatctgaatagtccctttccctgcacctctgtccatcgaggactcatcaggaggatactcaaataagcgcaaataataacagtcgcgtcagtatttgcggcagcgaaatggtttccctctccctcatttaccccatctgaggatagttttatgacagaaaaatggattgaagcagtcgccctttctgacatcccagaaggtgatgtcctcggcgtgactgtcgagggcaaggagctggcgttgtacgaagtggaaggcgaaatctacgctaccgacaacctgtgcacgcatggtgccgcccgcatgagcgatggttatctcgaggggcgagaaatcgaatgccccttgcatcaaggtcggtttgacgtttgtacaggcagagccctctgcgcccccgtgacagagaacatcaaaacatatgcagtcaagattgagaacctgcgcgtaatgattgatttgagcggagagttttaataggaggtaccccggaccctagcgcgtaactaccccaattccatcttttttaggtgaaaacatgaattacaaaaacaaaatattggtgagtgaatctgggctgacccaaaagcacctgattcatggcgatgaagaacttttccagcacgaactgagaaccatttttgcgcggaactggctttttctcactcatgacagcctgattccatcccccggcgactatgttaccgcaaaaatgggtattgacgaggtcatcgtctctcggcagagcgacggttcgattcgtgccttcctgaacgtttgtcggcaccgtggcaagacgctggttaacgcggaagccggcaatgccaaaggtttcgtttgcagctatcacggctggggcttcggctccaacggtgaactgcagagcgttccattcgaaaaagagctgtacggcgagtcgctcaacaaaaaatgtctggggttgaaagaagtcgctcgcgtggagagcttccatggcttcatctatggttgcttcgatcaggaggcccctcctcttatggactatctgggtgacgctgcttggtacctagagcccatcttcaaacattcaggcggtttagaactggtcggtcctccaggcaaggttgtgatcaaggccaactggaaggcacccgcggaaaactttgtgggggatgcataccacgtgggttggacgcacgcgtcttcgcttcgctcgggagagtctatcttcgcgtcgctcgctggcaacgcagtgctgccccctgaaggtgcaggcttgcaaatgacctccaaatacggcagcggcatgggtgtgttgtgggacggatattcaggcgtgcatagcgcagacttggttccggagttgatggcattcggcggctctaagcaggaaaggctgaacaaagaaattggcgatgttcgcgcccggatttatcgcagccacctcaactgcaccgttttcccgaacaacagcatgctgacctgctcgggtgttttcaaagtatggaacccgatcgacgcaaacaccaccgaggtctggacctacgccattgtcgaaaaagacatgcccgaggatctcaagcgccgcttggccgactcggttcagcgtacgtttgggcctgctggcttctgggaaagcgacgacaatgacaatatggaaacagcgtcgcaaaacggcaagaaatatcaatccagagatagtgatctgctttcaaaccttggtttcggtaaggacgtatacggcgacgcggtctatcctggcgtcgtcggcaaatcggcgatcggcgagaccagttatcgtggtttctaccgggcttaccaggcacacgtcagcagctccaactgggctgagttcgaggatgcctctagtacttggcataccgaactgacgaagactactgatcgctaacagacgagtcgaccatgatgatcaatattcaagaagacaagctggtctccgcccacgacgcccaagagtttcttcgtttcttcaattgccacgacgcggctttgcaacaagaagccaccacgctgctgaaccgggaagcgcatctgttggacattcaggcttaccgggcttggttagagcactgcgtggggtcagaggttcaatatcaggtcatttcacgcgaactgcgcgccgcttccgagcgccgttataagctcaatgaagccatgaacgtttacaacgaaaattttcagcaactgaaagttcgaatcgagcatcaactggatccgcaaaactggagcaacagcccgaagctgcgctttactcgcttcatcaccaatgtccaggccgcaagggacgtagatgacgaagagctacttcacatccgctccaacgtcattctgcaccgggcacgacgtggcaatcaggtcgatgtcttctacgccgcccgggaagacaaatggaaacgtggcgaaggtggagtgcgaaaattggtccagcgattcgtggattacccagagcgcatacttcagacgcacaatctgatggtctttctgtgatccagtgaccacttttacaaatggtgactgctaccgcggtcaccattaatcaaaagggaatgtaagtgtatgggcaatcaacaagtcgtttcgataaccggtgccggctcaggaatcggtctcgaactggttcgatcctttaagttggccggttattgcgtatccgctctcgtgcgaaacgaggagcaagaggcgcttctttgcaatgagttcaaggacgcactcgagatcgtagtgggcgatgtccgggaccacgcgacaaatgagaagctgataaagcagacaattgatagattcggtcatctcgattgtttcatcgcaaatgccggtatttgggattacatgctgaatatcgaagagccttgggagaaaatctcgagcagttttgacgaaatattcgacatcaatgtcaagagctatttcagtggcatcagtgccgccctgcccgagctgaaaaagactaacggatcagtggtgatgaccgcttcggtgtcgtcccatgcggtcggtggtggtggttcttgctacatcgccagcaagcatgcggtgctgggtatggtcaaggctttggcctacgaattggcccccgaaattcgcgtgaacgctgtttcgcctggcggcaccgtgacgtctctgtgcggtcccgctagcgccggtttcgacaaaatgcacatgaaagacatgcccggcatcgacgatatgatcaaaggtctcacacctcttgggtttgcagccaagcccgaagacgtggtggcaccctatctgttgctggcttcgcgaaagcaaggaaaattcatcactggcaccgtgattagcattgatggcggtatggcgctcggtcgcaagtgagcttgtagccgatcagaagttagacacattttcaggtgacgccccatgaagacaaaactgtttatcaacaacgcctggatcgattccagtgaccagcagaccttcgagcgcaagcaccccgtcagcagcgaggtgatgactgagagcgcaaacgccacggtgacggacgcgataaaggcggcgcaagcggccgaggaggcattcaagacctggaaggacgtcggaccttcggagcgtcgccgccttctcctgaaggtcgccgatgtcatggaaagtaaaacaccgaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgggaggctgcctcgctggccacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgaccctacgtcagccggtcggtccgatcctaagcatcgttccatggaacggcaccgcagtgctggcggcacgagccatcgcttatccgctggtctgtggcaacacggtagtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctggcgtgctcaactacctcaactcttcgcctgaccgttcgcccgagatcgccgacgcactgatctccgccaaggagatccgccgcatcaacttcacgggttccacccgcgtgggcagcattatcgcgcagaaggccgcgcaacacctcaagcgctgcctactggagctcggcggcaagtccccgcttattgttttagatgacgcagacatcaacgcggcggtaaaggcagcggtgttcggtagctttctattccaaggtcagatctgcatgtccactgagcgcttggtggttgatgagaagatcgccgacgaatttgtcgctaggtttgtcgaaaaaactgagcgcttgagcgtgggcgacccgtgcctaactggcgactgcatcatcggcccgatggtctcgccaaattcgggcgagcggatcaatggtttgttcaaggacgcgatagataaaggggccaaagttgtttgcggcggcatggcccagggtgcggtcatgccggccacgatcctggatcacgtcaaatctgacatgcggatctacgatgaggagacctttggtcccatcaccgtggtgatccgttgcaaaggcgaagcagaggccatccgcattgccaacgacagcgtttatggtctgtcgtcgggcgtgtttggccgcgacatcaaccgtgctctgcgagtgggtatgtccatcgaatatggttgcgtacacatcaacggctcgaccgtccagaacgaggcgcaggctccttacggaggtaccaagaacaccggctacgggcgcttcgacggccgtgccgtgatcgacgagttcacagagctcaagtggctgaccattgaaccattcgagcagcaatatcccttctgaaagcactaactccaaggaatcaaactatgagtaagcaagctgcagttatcgagctcggatacatgggcatctcggtcaaggatcctgatgcgtggaaatcgtttgccatgaatatgctgggtctgcaagttctggatgagggtgagaaggaccgtttctatctgcggatggattactggcaccatcggatcgtagttcatcacagcgcagaggacgacttggaatacctaggctggcgtgtagccggcaagccggagttcgaagctctggggcaaaagctcattgatgccggttacaagatccgtgtctgcgacaaagttgaggctcaggagcgtatggtgttgggcctgatgaagacagaagatccaggcggcaacccgaccgagatattctgggggccccggatcgacatgagcaatccgttccatcccggccgccctctgcacggcaagtttgtgaccggtgaccagggcttgggccattgcatcgttcgccaaaccgacgtcgctgcggcccataagttctacagcctgctgggcttccgtggggacgtcgaataccgcattccattgcccaatggcatgactgccgaactgtcgttcatgcattgcaacgcccgtgatcactccattgcgtttggtgccatgcccgctgccaagcgcctcaatcacttgatgcttgagtacacccatatggaagacttgggatatacgcatcaacagttcgtgaagaacgaaattgacattgccttgcagcttggcattcacgccaacgacaaggcgctgacgttctacggcgcaacgccttcgggctggcttattgagcccggctggcgaggtgctacggccatagacgaagcggagtattacgtcggcgacatcttcggccatggcgtcgaggcacctggatatggcctggatgtaaaactgagttaaaagcgcttatgcgcacattgggtttctgacgttttctgcttcctatacagcacaataacaataacaataacaataacaactaagtggaatataataatgataaaaaaactgttccttacgtgctttgtttactgttcactcagcaccgcatgggccgaagaatcaccttggacataccgcatcggcatgagtaatattgcttttgatacaagcgccaaagtgtacttggatggccaccgtgtgccgggaggaagcgctgacgcgagcaataacaacgcgctcacattcgacttcggctacgccatcaacgaccagtggaatgcacgtttgattgtcggtattccgcctacaactaaagttaagggcgcaggcacacttccgggcattcagctggggaaaataacttatgctccaacaatattaacgttaaactataacctcccagcttttgggcccgttcgtcctcacatcggtgcgggagtcaattacacgcggattttggaaagcaaggacgctaatctaaaatcattcgatgccgaccacgcttggtcccccgcgctgcatgttggtgccgatattgacgtgagtcgcaactggttcgtcagcattgatatccggaagttatacctgaaaaccgacgcatcaggttacttagggccacaggaggctaaagcaaaggtaactcttgacccattgataacatcgatcgcgatcggacgccaattctgatgaccccttttaaggctctctatctatctaactgcaaagggtatttttatgttgaataaagttattaaaaccacgcgtcttaccgctgaagatatcaacggtgcctggactataatgcccacaccgtcgacgcctgatgcttctgattggcgcagcactaacactgtggacttagacgagactgcccgcatagttgaagagctgattgcagctggtgtcaacggtattttaagtatgggtacttttggtgagtgcgccacgttgacctgggaggagaaacgtgattatgtttcgacggttgtcgagaccattcgtggtcgtgtgccttatttctgtggcacgacggccctgaatacccgagaagtcatccgccagacccgagagcttatcgatattggcgctaacggcaccatgctaggcgtgccgatgtgggtgaagatggacctgcccacagcggtccagttctatcgtgatgttgcaggcgcggtaccggaggctgccattgcgatttacgccaaccccgaagcattcaaattcgacttccctcgcccattttgggcagagatgtccaaaattcctcaggtagtgactgccaagtatctaggcatcggaatgcttgacttggacctgaaattggcgcctaacatccgcttccttccacacgaggacgactattacgcggccgcacgcatcaatcccgagcgcataaccgcgttctggtcaagcggggccatgtgcggcccggctaccgctatcatgttgcgtgatgaagtggagcgggccaagagtaccggtgactggatcaaggccaaagccatctccgatgatatgcgtgcagccgattcgacattgtttccgcgtggcgacttttcggagttctcgaagtataacatcgggcttgaaaaggcacggatggacgcggctggttggctcaaggctggtccctgccgtcctccctacaatcttgttccagaagattacctcgttggtgcacagaaatcaggcaaggcgtgggccgcgctgcacgctaaatacagtaaataattaaagtagttcacctccgcaggcctgagcgcgagggttacgaacacgctgagcggtgcgggaagtaagtgagttaaagctcatctcttgcgccaggcactgctagatcagcaaagttagctgatctggcagtctcgaaaattttggcgaaagctgatcttaggaatgcgggataaaggcagtacaccgtaacgatgggggtgtgccgttcatgttgaacgacgccgctattgcgccgacttctcttcttcggagtgtttgattgtgattgtcgatttttatttcgattttttgagtccgttctcttacttggccaatcagcgcttgtcaaagcttgcgcaagattatggccttaccattcgttataacgcgatcgatttggcgcgggtcaaaatagccattggaaacgttggtccatccaatcgcgacttgaaagtcaaattggactatttgaaagtagatttgcaacggtgggcccagctttacggaataccgctggtattcccagctaactacaacagccgacggatgaatattgggttttattactcgggggccgaggcgcaggccgctgcctatgtgaatgtagtatttaatgcggtttggggagaaggcatagctccagatttggaaagcttgcctgccctggtatctgaaaagctaggctgggatcgtagtgctttcgagcactttctcagcagcaatgccgcaacagagaggtatgacgagcagacacatgccgccatcgagcgcaaggtgttcggtgtgccaacgatgtttttgggcgatgaaatgtggtgggggaacgaccgtctattcatgctcgagagcgcaatggggcgcttgtgccggcaaaatgccgatttaagtagttgatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z