LOCUS BBa_K957006 9831 bp DNA linear 22-Nov-2024 DEFINITION Constitutive expression of nah Operon with oriT ACCESSION BBa_K957006 VERSION BBa_K957006.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23114 CDS 56..1042 /label=nahAa CDS 1186..1509 /label=nahAb CDS 1571..2920 /label=nahAc CDS 2935..3519 /label=nahAd CDS 3589..4368 /label=nahB CDS 4414..5865 /label=nahF CDS 5892..6800 /label=nahC CDS 6897..7529 /label=nahQ CDS 7578..8573 /label=nahE CDS 8838..9449 /label=nahD misc_feature 1910..1915 /label=PstI cut site misc_feature 5905..5910 /label=PstI cut site misc_feature 8208..8215 /label=NotI cut site CDS 44..9452 /label=BBa_K957004 misc_feature 1..9452 /label=BBa_K957005 oriT 9461..9831 /label=BBa_J01003 ORIGIN 1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagtaagccg gatttatgga 61 acttctcata cagccaaaca atcgcctcat tagctttagt cccggcgcca accttctgga 121 agtgcttcgc gaaaacggtg tcgctatttc ctacagttgt atgtctgggc gttgcggaac 181 ctgccgctgt cgggttacag atggcagtgt aattgattcg ggggcgggaa gcgggttacc 241 aaacctcgtg gacgagcatt atgtgctcgc ctgtcagtca gtacttactc acaattgcgc 301 gatcgaaatc ccagaaaccg acgaaatcgt cacccacccg gcgagaatca tcaagggcac 361 tgtggtcgcc gtcgagtcgc ccactcacga tatccgtcgc ctacgcgtac gcctcgctaa 421 gcccttcgag ttctcacccg gacagtacgc gacattgcag ttcagtcctg agcatgcgcg 481 tccgtattca atggcaggtc tgccagatga ccaagaaatg gagttccaca tacgcaaggt 541 gccgggtggg cgcgtaacgg agtatgtttt cgagcacgtc cgcgaaggta caagcatcaa 601 gttgagcggg ccacttggta cggcttattt gcgtcagaac cacaccgggc cgatgctctg 661 tgtgggcggt gggaccggac tagcaccggt gctgtcgatt gttcgcggcg cgctgaagtt 721 gggtatgaca aaccccatcc tcctttattt cggagtgcgc agtcagcaag acctctacga 781 cgcagagcga ttgcacaaac tcgccgctga tcaccctcaa ctgaccgtac acacggtaat 841 cgcaatgggc ccgattaatg agagtcagcg agccggtcta gttaccgatg tgatcgaaaa 901 agacatcatt tcgctggctg ggtggagggc ctacctgtgc ggcgcaccag cgatggttga 961 agcgctttgc accgttacca agcatcttgg aatatcaccc gaacatattt atgccgatgc 1021 cttctatccc ggtggaatct gaatagtccc tttccctgca cctctgtcca tcgaggactc 1081 atcaggagga tactcaaata agcgcaaata ataacagtcg cgtcagtatt tgcggcagcg 1141 aaatggtttc cctctccctc atttacccca tctgaggata gttttatgac agaaaaatgg 1201 attgaagcag tcgccctttc tgacatccca gaaggtgatg tcctcggcgt gactgtcgag 1261 ggcaaggagc tggcgttgta cgaagtggaa ggcgaaatct acgctaccga caacctgtgc 1321 acgcatggtg ccgcccgcat gagcgatggt tatctcgagg ggcgagaaat cgaatgcccc 1381 ttgcatcaag gtcggtttga cgtttgtaca ggcagagccc tctgcgcccc cgtgacagag 1441 aacatcaaaa catatgcagt caagattgag aacctgcgcg taatgattga tttgagcgga 1501 gagttttaat aggaggtacc ccggacccta gcgcgtaact accccaattc catctttttt 1561 aggtgaaaac atgaattaca aaaacaaaat attggtgagt gaatctgggc tgacccaaaa 1621 gcacctgatt catggcgatg aagaactttt ccagcacgaa ctgagaacca tttttgcgcg 1681 gaactggctt tttctcactc atgacagcct gattccatcc cccggcgact atgttaccgc 1741 aaaaatgggt attgacgagg tcatcgtctc tcggcagagc gacggttcga ttcgtgcctt 1801 cctgaacgtt tgtcggcacc gtggcaagac gctggttaac gcggaagccg gcaatgccaa 1861 aggtttcgtt tgcagctatc acggctgggg cttcggctcc aacggtgaac tgcagagcgt 1921 tccattcgaa aaagagctgt acggcgagtc gctcaacaaa aaatgtctgg ggttgaaaga 1981 agtcgctcgc gtggagagct tccatggctt catctatggt tgcttcgatc aggaggcccc 2041 tcctcttatg gactatctgg gtgacgctgc ttggtaccta gagcccatct tcaaacattc 2101 aggcggttta gaactggtcg gtcctccagg caaggttgtg atcaaggcca actggaaggc 2161 acccgcggaa aactttgtgg gggatgcata ccacgtgggt tggacgcacg cgtcttcgct 2221 tcgctcggga gagtctatct tcgcgtcgct cgctggcaac gcagtgctgc cccctgaagg 2281 tgcaggcttg caaatgacct ccaaatacgg cagcggcatg ggtgtgttgt gggacggata 2341 ttcaggcgtg catagcgcag acttggttcc ggagttgatg gcattcggcg gctctaagca 2401 ggaaaggctg aacaaagaaa ttggcgatgt tcgcgcccgg atttatcgca gccacctcaa 2461 ctgcaccgtt ttcccgaaca acagcatgct gacctgctcg ggtgttttca aagtatggaa 2521 cccgatcgac gcaaacacca ccgaggtctg gacctacgcc attgtcgaaa aagacatgcc 2581 cgaggatctc aagcgccgct tggccgactc ggttcagcgt acgtttgggc ctgctggctt 2641 ctgggaaagc gacgacaatg acaatatgga aacagcgtcg caaaacggca agaaatatca 2701 atccagagat agtgatctgc tttcaaacct tggtttcggt aaggacgtat acggcgacgc 2761 ggtctatcct ggcgtcgtcg gcaaatcggc gatcggcgag accagttatc gtggtttcta 2821 ccgggcttac caggcacacg tcagcagctc caactgggct gagttcgagg atgcctctag 2881 tacttggcat accgaactga cgaagactac tgatcgctaa cagacgagtc gaccatgatg 2941 atcaatattc aagaagacaa gctggtctcc gcccacgacg cccaagagtt tcttcgtttc 3001 ttcaattgcc acgacgcggc tttgcaacaa gaagccacca cgctgctgaa ccgggaagcg 3061 catctgttgg acattcaggc ttaccgggct tggttagagc actgcgtggg gtcagaggtt 3121 caatatcagg tcatttcacg cgaactgcgc gccgcttccg agcgccgtta taagctcaat 3181 gaagccatga acgtttacaa cgaaaatttt cagcaactga aagttcgaat cgagcatcaa 3241 ctggatccgc aaaactggag caacagcccg aagctgcgct ttactcgctt catcaccaat 3301 gtccaggccg caagggacgt agatgacgaa gagctacttc acatccgctc caacgtcatt 3361 ctgcaccggg cacgacgtgg caatcaggtc gatgtcttct acgccgcccg ggaagacaaa 3421 tggaaacgtg gcgaaggtgg agtgcgaaaa ttggtccagc gattcgtgga ttacccagag 3481 cgcatacttc agacgcacaa tctgatggtc tttctgtgat ccagtgacca cttttacaaa 3541 tggtgactgc taccgcggtc accattaatc aaaagggaat gtaagtgtat gggcaatcaa 3601 caagtcgttt cgataaccgg tgccggctca ggaatcggtc tcgaactggt tcgatccttt 3661 aagttggccg gttattgcgt atccgctctc gtgcgaaacg aggagcaaga ggcgcttctt 3721 tgcaatgagt tcaaggacgc actcgagatc gtagtgggcg atgtccggga ccacgcgaca 3781 aatgagaagc tgataaagca gacaattgat agattcggtc atctcgattg tttcatcgca 3841 aatgccggta tttgggatta catgctgaat atcgaagagc cttgggagaa aatctcgagc 3901 agttttgacg aaatattcga catcaatgtc aagagctatt tcagtggcat cagtgccgcc 3961 ctgcccgagc tgaaaaagac taacggatca gtggtgatga ccgcttcggt gtcgtcccat 4021 gcggtcggtg gtggtggttc ttgctacatc gccagcaagc atgcggtgct gggtatggtc 4081 aaggctttgg cctacgaatt ggcccccgaa attcgcgtga acgctgtttc gcctggcggc 4141 accgtgacgt ctctgtgcgg tcccgctagc gccggtttcg acaaaatgca catgaaagac 4201 atgcccggca tcgacgatat gatcaaaggt ctcacacctc ttgggtttgc agccaagccc 4261 gaagacgtgg tggcacccta tctgttgctg gcttcgcgaa agcaaggaaa attcatcact 4321 ggcaccgtga ttagcattga tggcggtatg gcgctcggtc gcaagtgagc ttgtagccga 4381 tcagaagtta gacacatttt caggtgacgc cccatgaaga caaaactgtt tatcaacaac 4441 gcctggatcg attccagtga ccagcagacc ttcgagcgca agcaccccgt cagcagcgag 4501 gtgatgactg agagcgcaaa cgccacggtg acggacgcga taaaggcggc gcaagcggcc 4561 gaggaggcat tcaagacctg gaaggacgtc ggaccttcgg agcgtcgccg ccttctcctg 4621 aaggtcgccg atgtcatgga aagtaaaaca ccgaagttca tcgaagtgat ggccatggag 4681 gtgggagctt ccgccctttg ggccggattc aacgtccatg cgtctgccaa tgtgttccgg 4741 gaggctgcct cgctggccac ccaaattcag ggtgaaacca tcccaacgga caaagccgaa 4801 acgctctcaa tgaccctacg tcagccggtc ggtccgatcc taagcatcgt tccatggaac 4861 ggcaccgcag tgctggcggc acgagccatc gcttatccgc tggtctgtgg caacacggta 4921 gtgttcaaag gctctgaatt tagtcccgcg acgcatgccc tgatcaccca gtgcgtgcag 4981 gaagccgggc tgcccgctgg cgtgctcaac tacctcaact cttcgcctga ccgttcgccc 5041 gagatcgccg acgcactgat ctccgccaag gagatccgcc gcatcaactt cacgggttcc 5101 acccgcgtgg gcagcattat cgcgcagaag gccgcgcaac acctcaagcg ctgcctactg 5161 gagctcggcg gcaagtcccc gcttattgtt ttagatgacg cagacatcaa cgcggcggta 5221 aaggcagcgg tgttcggtag ctttctattc caaggtcaga tctgcatgtc cactgagcgc 5281 ttggtggttg atgagaagat cgccgacgaa tttgtcgcta ggtttgtcga aaaaactgag 5341 cgcttgagcg tgggcgaccc gtgcctaact ggcgactgca tcatcggccc gatggtctcg 5401 ccaaattcgg gcgagcggat caatggtttg ttcaaggacg cgatagataa aggggccaaa 5461 gttgtttgcg gcggcatggc ccagggtgcg gtcatgccgg ccacgatcct ggatcacgtc 5521 aaatctgaca tgcggatcta cgatgaggag acctttggtc ccatcaccgt ggtgatccgt 5581 tgcaaaggcg aagcagaggc catccgcatt gccaacgaca gcgtttatgg tctgtcgtcg 5641 ggcgtgtttg gccgcgacat caaccgtgct ctgcgagtgg gtatgtccat cgaatatggt 5701 tgcgtacaca tcaacggctc gaccgtccag aacgaggcgc aggctcctta cggaggtacc 5761 aagaacaccg gctacgggcg cttcgacggc cgtgccgtga tcgacgagtt cacagagctc 5821 aagtggctga ccattgaacc attcgagcag caatatccct tctgaaagca ctaactccaa 5881 ggaatcaaac tatgagtaag caagctgcag ttatcgagct cggatacatg ggcatctcgg 5941 tcaaggatcc tgatgcgtgg aaatcgtttg ccatgaatat gctgggtctg caagttctgg 6001 atgagggtga gaaggaccgt ttctatctgc ggatggatta ctggcaccat cggatcgtag 6061 ttcatcacag cgcagaggac gacttggaat acctaggctg gcgtgtagcc ggcaagccgg 6121 agttcgaagc tctggggcaa aagctcattg atgccggtta caagatccgt gtctgcgaca 6181 aagttgaggc tcaggagcgt atggtgttgg gcctgatgaa gacagaagat ccaggcggca 6241 acccgaccga gatattctgg gggccccgga tcgacatgag caatccgttc catcccggcc 6301 gccctctgca cggcaagttt gtgaccggtg accagggctt gggccattgc atcgttcgcc 6361 aaaccgacgt cgctgcggcc cataagttct acagcctgct gggcttccgt ggggacgtcg 6421 aataccgcat tccattgccc aatggcatga ctgccgaact gtcgttcatg cattgcaacg 6481 cccgtgatca ctccattgcg tttggtgcca tgcccgctgc caagcgcctc aatcacttga 6541 tgcttgagta cacccatatg gaagacttgg gatatacgca tcaacagttc gtgaagaacg 6601 aaattgacat tgccttgcag cttggcattc acgccaacga caaggcgctg acgttctacg 6661 gcgcaacgcc ttcgggctgg cttattgagc ccggctggcg aggtgctacg gccatagacg 6721 aagcggagta ttacgtcggc gacatcttcg gccatggcgt cgaggcacct ggatatggcc 6781 tggatgtaaa actgagttaa aagcgcttat gcgcacattg ggtttctgac gttttctgct 6841 tcctatacag cacaataaca ataacaataa caataacaac taagtggaat ataataatga 6901 taaaaaaact gttccttacg tgctttgttt actgttcact cagcaccgca tgggccgaag 6961 aatcaccttg gacataccgc atcggcatga gtaatattgc ttttgataca agcgccaaag 7021 tgtacttgga tggccaccgt gtgccgggag gaagcgctga cgcgagcaat aacaacgcgc 7081 tcacattcga cttcggctac gccatcaacg accagtggaa tgcacgtttg attgtcggta 7141 ttccgcctac aactaaagtt aagggcgcag gcacacttcc gggcattcag ctggggaaaa 7201 taacttatgc tccaacaata ttaacgttaa actataacct cccagctttt gggcccgttc 7261 gtcctcacat cggtgcggga gtcaattaca cgcggatttt ggaaagcaag gacgctaatc 7321 taaaatcatt cgatgccgac cacgcttggt cccccgcgct gcatgttggt gccgatattg 7381 acgtgagtcg caactggttc gtcagcattg atatccggaa gttatacctg aaaaccgacg 7441 catcaggtta cttagggcca caggaggcta aagcaaaggt aactcttgac ccattgataa 7501 catcgatcgc gatcggacgc caattctgat gacccctttt aaggctctct atctatctaa 7561 ctgcaaaggg tatttttatg ttgaataaag ttattaaaac cacgcgtctt accgctgaag 7621 atatcaacgg tgcctggact ataatgccca caccgtcgac gcctgatgct tctgattggc 7681 gcagcactaa cactgtggac ttagacgaga ctgcccgcat agttgaagag ctgattgcag 7741 ctggtgtcaa cggtatttta agtatgggta cttttggtga gtgcgccacg ttgacctggg 7801 aggagaaacg tgattatgtt tcgacggttg tcgagaccat tcgtggtcgt gtgccttatt 7861 tctgtggcac gacggccctg aatacccgag aagtcatccg ccagacccga gagcttatcg 7921 atattggcgc taacggcacc atgctaggcg tgccgatgtg ggtgaagatg gacctgccca 7981 cagcggtcca gttctatcgt gatgttgcag gcgcggtacc ggaggctgcc attgcgattt 8041 acgccaaccc cgaagcattc aaattcgact tccctcgccc attttgggca gagatgtcca 8101 aaattcctca ggtagtgact gccaagtatc taggcatcgg aatgcttgac ttggacctga 8161 aattggcgcc taacatccgc ttccttccac acgaggacga ctattacgcg gccgcacgca 8221 tcaatcccga gcgcataacc gcgttctggt caagcggggc catgtgcggc ccggctaccg 8281 ctatcatgtt gcgtgatgaa gtggagcggg ccaagagtac cggtgactgg atcaaggcca 8341 aagccatctc cgatgatatg cgtgcagccg attcgacatt gtttccgcgt ggcgactttt 8401 cggagttctc gaagtataac atcgggcttg aaaaggcacg gatggacgcg gctggttggc 8461 tcaaggctgg tccctgccgt cctccctaca atcttgttcc agaagattac ctcgttggtg 8521 cacagaaatc aggcaaggcg tgggccgcgc tgcacgctaa atacagtaaa taattaaagt 8581 agttcacctc cgcaggcctg agcgcgaggg ttacgaacac gctgagcggt gcgggaagta 8641 agtgagttaa agctcatctc ttgcgccagg cactgctaga tcagcaaagt tagctgatct 8701 ggcagtctcg aaaattttgg cgaaagctga tcttaggaat gcgggataaa ggcagtacac 8761 cgtaacgatg ggggtgtgcc gttcatgttg aacgacgccg ctattgcgcc gacttctctt 8821 cttcggagtg tttgattgtg attgtcgatt tttatttcga ttttttgagt ccgttctctt 8881 acttggccaa tcagcgcttg tcaaagcttg cgcaagatta tggccttacc attcgttata 8941 acgcgatcga tttggcgcgg gtcaaaatag ccattggaaa cgttggtcca tccaatcgcg 9001 acttgaaagt caaattggac tatttgaaag tagatttgca acggtgggcc cagctttacg 9061 gaataccgct ggtattccca gctaactaca acagccgacg gatgaatatt gggttttatt 9121 actcgggggc cgaggcgcag gccgctgcct atgtgaatgt agtatttaat gcggtttggg 9181 gagaaggcat agctccagat ttggaaagct tgcctgccct ggtatctgaa aagctaggct 9241 gggatcgtag tgctttcgag cactttctca gcagcaatgc cgcaacagag aggtatgacg 9301 agcagacaca tgccgccatc gagcgcaagg tgttcggtgt gccaacgatg tttttgggcg 9361 atgaaatgtg gtgggggaac gaccgtctat tcatgctcga gagcgcaatg gggcgcttgt 9421 gccggcaaaa tgccgattta agtagttgat gatactagag gccgcctttt cctcaatcgc 9481 tcttcgttcg tctggaaggc agtacacctt gataggtggg ctgcccttcc tggttggctt 9541 ggtttcatca gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg 9601 cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca 9661 cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca 9721 aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata 9781 taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa a //