BBa_K957004 1 BBa_K957004 nah Operon for degradation of naphthalene to salicylate 2012-09-24T11:00:00Z 2015-05-08T01:13:49Z From NAH7 plasmid of Pseudomonas putida G7 (provided by Dr. Gene Madsen, Cornell University Department of Microbiology). One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene. false false _1228_ 0 13504 9 It's complicated true Three internal BioBrick cut sites (one NotI, two PstI) remain in this version of the part. However, the utility of the part is not restricted, since digestion with EcoRI and SpeI facilitates standard molecular cloning. false Dylan Patrick Webster annotation2199014 1 NotI cut site range2199014 1 8165 8172 annotation2199013 1 PstI cut site range2199013 1 5862 5867 annotation2199010 1 nahE range2199010 1 7535 8530 annotation2199006 1 nahB range2199006 1 3546 4325 annotation2199004 1 nahAc range2199004 1 1528 2877 annotation2199009 1 nahQ range2199009 1 6854 7486 annotation2199007 1 nahF range2199007 1 4371 5822 annotation2199008 1 nahC range2199008 1 5849 6757 annotation2199011 1 nahD range2199011 1 8795 9406 annotation2199012 1 PstI cut site range2199012 1 1867 1872 annotation2199002 1 nahAa range2199002 1 13 999 annotation2199003 1 nahAb range2199003 1 1143 1466 annotation2199005 1 nahAd range2199005 1 2892 3476 BBa_J01003 1 OriTR OriT-R (Origin of transfer for the R-plasmid nic region) 2005-10-17T11:00:00Z 2015-08-31T04:08:11Z Released HQ 2013 OriTR, the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins. false false _13_ 0 395 13 In stock false true Golden Bear BBa_K957005 1 BBa_K957005 Constitutive expression of nah Operon for degradation of naphthalene to salicylate 2012-09-24T11:00:00Z 2015-05-08T01:13:49Z From NAH7 plasmid of Pseudomonas putida G7 (provided by Dr. Gene Madsen, Cornell University Department of Microbiology). One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene. false false _1228_ 0 13504 9 It's complicated true Three internal BioBrick cut sites (one NotI, two PstI) remain in this version of the part. However, the utility of the part is not restricted, since digestion with EcoRI and SpeI facilitates standard molecular cloning. false Dylan Patrick Webster component2199029 1 BBa_K957004 component2199015 1 BBa_J23114 annotation2199015 1 BBa_J23114 range2199015 1 1 35 annotation2199029 1 BBa_K957004 range2199029 1 44 9452 BBa_K957006 1 BBa_K957006 Constitutive expression of nah Operon with oriT 2012-09-28T11:00:00Z 2015-05-08T01:13:49Z From NAH7 plasmid of Pseudomonas putida G7 (provided by Dr. Gene Madsen, Cornell University Department of Microbiology). One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene. Also included in this BioBrick part is an oriT sequence. When cloned into an existing non-conjugative BioBrick vector, the resulting plasmid may be inserted into a host via conjugation. false false _1228_ 0 13504 9 Not in stock false Three internal BioBrick cut sites (one NotI, two PstI) remain in this version of the part. However, the utility of the part is not restricted, since digestion with EcoRI and SpeI facilitates standard molecular cloning. false Dylan Patrick Webster component2204251 1 BBa_K957005 component2204252 1 BBa_J01003 annotation2204251 1 BBa_K957005 range2204251 1 1 9452 annotation2204252 1 BBa_J01003 range2204252 1 9461 9831 BBa_J23114 1 BBa_J23114 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K957004_sequence 1 taagccggatttatggaacttctcatacagccaaacaatcgcctcattagctttagtcccggcgccaaccttctggaagtgcttcgcgaaaacggtgtcgctatttcctacagttgtatgtctgggcgttgcggaacctgccgctgtcgggttacagatggcagtgtaattgattcgggggcgggaagcgggttaccaaacctcgtggacgagcattatgtgctcgcctgtcagtcagtacttactcacaattgcgcgatcgaaatcccagaaaccgacgaaatcgtcacccacccggcgagaatcatcaagggcactgtggtcgccgtcgagtcgcccactcacgatatccgtcgcctacgcgtacgcctcgctaagcccttcgagttctcacccggacagtacgcgacattgcagttcagtcctgagcatgcgcgtccgtattcaatggcaggtctgccagatgaccaagaaatggagttccacatacgcaaggtgccgggtgggcgcgtaacggagtatgttttcgagcacgtccgcgaaggtacaagcatcaagttgagcgggccacttggtacggcttatttgcgtcagaaccacaccgggccgatgctctgtgtgggcggtgggaccggactagcaccggtgctgtcgattgttcgcggcgcgctgaagttgggtatgacaaaccccatcctcctttatttcggagtgcgcagtcagcaagacctctacgacgcagagcgattgcacaaactcgccgctgatcaccctcaactgaccgtacacacggtaatcgcaatgggcccgattaatgagagtcagcgagccggtctagttaccgatgtgatcgaaaaagacatcatttcgctggctgggtggagggcctacctgtgcggcgcaccagcgatggttgaagcgctttgcaccgttaccaagcatcttggaatatcacccgaacatatttatgccgatgccttctatcccggtggaatctgaatagtccctttccctgcacctctgtccatcgaggactcatcaggaggatactcaaataagcgcaaataataacagtcgcgtcagtatttgcggcagcgaaatggtttccctctccctcatttaccccatctgaggatagttttatgacagaaaaatggattgaagcagtcgccctttctgacatcccagaaggtgatgtcctcggcgtgactgtcgagggcaaggagctggcgttgtacgaagtggaaggcgaaatctacgctaccgacaacctgtgcacgcatggtgccgcccgcatgagcgatggttatctcgaggggcgagaaatcgaatgccccttgcatcaaggtcggtttgacgtttgtacaggcagagccctctgcgcccccgtgacagagaacatcaaaacatatgcagtcaagattgagaacctgcgcgtaatgattgatttgagcggagagttttaataggaggtaccccggaccctagcgcgtaactaccccaattccatcttttttaggtgaaaacatgaattacaaaaacaaaatattggtgagtgaatctgggctgacccaaaagcacctgattcatggcgatgaagaacttttccagcacgaactgagaaccatttttgcgcggaactggctttttctcactcatgacagcctgattccatcccccggcgactatgttaccgcaaaaatgggtattgacgaggtcatcgtctctcggcagagcgacggttcgattcgtgccttcctgaacgtttgtcggcaccgtggcaagacgctggttaacgcggaagccggcaatgccaaaggtttcgtttgcagctatcacggctggggcttcggctccaacggtgaactgcagagcgttccattcgaaaaagagctgtacggcgagtcgctcaacaaaaaatgtctggggttgaaagaagtcgctcgcgtggagagcttccatggcttcatctatggttgcttcgatcaggaggcccctcctcttatggactatctgggtgacgctgcttggtacctagagcccatcttcaaacattcaggcggtttagaactggtcggtcctccaggcaaggttgtgatcaaggccaactggaaggcacccgcggaaaactttgtgggggatgcataccacgtgggttggacgcacgcgtcttcgcttcgctcgggagagtctatcttcgcgtcgctcgctggcaacgcagtgctgccccctgaaggtgcaggcttgcaaatgacctccaaatacggcagcggcatgggtgtgttgtgggacggatattcaggcgtgcatagcgcagacttggttccggagttgatggcattcggcggctctaagcaggaaaggctgaacaaagaaattggcgatgttcgcgcccggatttatcgcagccacctcaactgcaccgttttcccgaacaacagcatgctgacctgctcgggtgttttcaaagtatggaacccgatcgacgcaaacaccaccgaggtctggacctacgccattgtcgaaaaagacatgcccgaggatctcaagcgccgcttggccgactcggttcagcgtacgtttgggcctgctggcttctgggaaagcgacgacaatgacaatatggaaacagcgtcgcaaaacggcaagaaatatcaatccagagatagtgatctgctttcaaaccttggtttcggtaaggacgtatacggcgacgcggtctatcctggcgtcgtcggcaaatcggcgatcggcgagaccagttatcgtggtttctaccgggcttaccaggcacacgtcagcagctccaactgggctgagttcgaggatgcctctagtacttggcataccgaactgacgaagactactgatcgctaacagacgagtcgaccatgatgatcaatattcaagaagacaagctggtctccgcccacgacgcccaagagtttcttcgtttcttcaattgccacgacgcggctttgcaacaagaagccaccacgctgctgaaccgggaagcgcatctgttggacattcaggcttaccgggcttggttagagcactgcgtggggtcagaggttcaatatcaggtcatttcacgcgaactgcgcgccgcttccgagcgccgttataagctcaatgaagccatgaacgtttacaacgaaaattttcagcaactgaaagttcgaatcgagcatcaactggatccgcaaaactggagcaacagcccgaagctgcgctttactcgcttcatcaccaatgtccaggccgcaagggacgtagatgacgaagagctacttcacatccgctccaacgtcattctgcaccgggcacgacgtggcaatcaggtcgatgtcttctacgccgcccgggaagacaaatggaaacgtggcgaaggtggagtgcgaaaattggtccagcgattcgtggattacccagagcgcatacttcagacgcacaatctgatggtctttctgtgatccagtgaccacttttacaaatggtgactgctaccgcggtcaccattaatcaaaagggaatgtaagtgtatgggcaatcaacaagtcgtttcgataaccggtgccggctcaggaatcggtctcgaactggttcgatcctttaagttggccggttattgcgtatccgctctcgtgcgaaacgaggagcaagaggcgcttctttgcaatgagttcaaggacgcactcgagatcgtagtgggcgatgtccgggaccacgcgacaaatgagaagctgataaagcagacaattgatagattcggtcatctcgattgtttcatcgcaaatgccggtatttgggattacatgctgaatatcgaagagccttgggagaaaatctcgagcagttttgacgaaatattcgacatcaatgtcaagagctatttcagtggcatcagtgccgccctgcccgagctgaaaaagactaacggatcagtggtgatgaccgcttcggtgtcgtcccatgcggtcggtggtggtggttcttgctacatcgccagcaagcatgcggtgctgggtatggtcaaggctttggcctacgaattggcccccgaaattcgcgtgaacgctgtttcgcctggcggcaccgtgacgtctctgtgcggtcccgctagcgccggtttcgacaaaatgcacatgaaagacatgcccggcatcgacgatatgatcaaaggtctcacacctcttgggtttgcagccaagcccgaagacgtggtggcaccctatctgttgctggcttcgcgaaagcaaggaaaattcatcactggcaccgtgattagcattgatggcggtatggcgctcggtcgcaagtgagcttgtagccgatcagaagttagacacattttcaggtgacgccccatgaagacaaaactgtttatcaacaacgcctggatcgattccagtgaccagcagaccttcgagcgcaagcaccccgtcagcagcgaggtgatgactgagagcgcaaacgccacggtgacggacgcgataaaggcggcgcaagcggccgaggaggcattcaagacctggaaggacgtcggaccttcggagcgtcgccgccttctcctgaaggtcgccgatgtcatggaaagtaaaacaccgaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgggaggctgcctcgctggccacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgaccctacgtcagccggtcggtccgatcctaagcatcgttccatggaacggcaccgcagtgctggcggcacgagccatcgcttatccgctggtctgtggcaacacggtagtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctggcgtgctcaactacctcaactcttcgcctgaccgttcgcccgagatcgccgacgcactgatctccgccaaggagatccgccgcatcaacttcacgggttccacccgcgtgggcagcattatcgcgcagaaggccgcgcaacacctcaagcgctgcctactggagctcggcggcaagtccccgcttattgttttagatgacgcagacatcaacgcggcggtaaaggcagcggtgttcggtagctttctattccaaggtcagatctgcatgtccactgagcgcttggtggttgatgagaagatcgccgacgaatttgtcgctaggtttgtcgaaaaaactgagcgcttgagcgtgggcgacccgtgcctaactggcgactgcatcatcggcccgatggtctcgccaaattcgggcgagcggatcaatggtttgttcaaggacgcgatagataaaggggccaaagttgtttgcggcggcatggcccagggtgcggtcatgccggccacgatcctggatcacgtcaaatctgacatgcggatctacgatgaggagacctttggtcccatcaccgtggtgatccgttgcaaaggcgaagcagaggccatccgcattgccaacgacagcgtttatggtctgtcgtcgggcgtgtttggccgcgacatcaaccgtgctctgcgagtgggtatgtccatcgaatatggttgcgtacacatcaacggctcgaccgtccagaacgaggcgcaggctccttacggaggtaccaagaacaccggctacgggcgcttcgacggccgtgccgtgatcgacgagttcacagagctcaagtggctgaccattgaaccattcgagcagcaatatcccttctgaaagcactaactccaaggaatcaaactatgagtaagcaagctgcagttatcgagctcggatacatgggcatctcggtcaaggatcctgatgcgtggaaatcgtttgccatgaatatgctgggtctgcaagttctggatgagggtgagaaggaccgtttctatctgcggatggattactggcaccatcggatcgtagttcatcacagcgcagaggacgacttggaatacctaggctggcgtgtagccggcaagccggagttcgaagctctggggcaaaagctcattgatgccggttacaagatccgtgtctgcgacaaagttgaggctcaggagcgtatggtgttgggcctgatgaagacagaagatccaggcggcaacccgaccgagatattctgggggccccggatcgacatgagcaatccgttccatcccggccgccctctgcacggcaagtttgtgaccggtgaccagggcttgggccattgcatcgttcgccaaaccgacgtcgctgcggcccataagttctacagcctgctgggcttccgtggggacgtcgaataccgcattccattgcccaatggcatgactgccgaactgtcgttcatgcattgcaacgcccgtgatcactccattgcgtttggtgccatgcccgctgccaagcgcctcaatcacttgatgcttgagtacacccatatggaagacttgggatatacgcatcaacagttcgtgaagaacgaaattgacattgccttgcagcttggcattcacgccaacgacaaggcgctgacgttctacggcgcaacgccttcgggctggcttattgagcccggctggcgaggtgctacggccatagacgaagcggagtattacgtcggcgacatcttcggccatggcgtcgaggcacctggatatggcctggatgtaaaactgagttaaaagcgcttatgcgcacattgggtttctgacgttttctgcttcctatacagcacaataacaataacaataacaataacaactaagtggaatataataatgataaaaaaactgttccttacgtgctttgtttactgttcactcagcaccgcatgggccgaagaatcaccttggacataccgcatcggcatgagtaatattgcttttgatacaagcgccaaagtgtacttggatggccaccgtgtgccgggaggaagcgctgacgcgagcaataacaacgcgctcacattcgacttcggctacgccatcaacgaccagtggaatgcacgtttgattgtcggtattccgcctacaactaaagttaagggcgcaggcacacttccgggcattcagctggggaaaataacttatgctccaacaatattaacgttaaactataacctcccagcttttgggcccgttcgtcctcacatcggtgcgggagtcaattacacgcggattttggaaagcaaggacgctaatctaaaatcattcgatgccgaccacgcttggtcccccgcgctgcatgttggtgccgatattgacgtgagtcgcaactggttcgtcagcattgatatccggaagttatacctgaaaaccgacgcatcaggttacttagggccacaggaggctaaagcaaaggtaactcttgacccattgataacatcgatcgcgatcggacgccaattctgatgaccccttttaaggctctctatctatctaactgcaaagggtatttttatgttgaataaagttattaaaaccacgcgtcttaccgctgaagatatcaacggtgcctggactataatgcccacaccgtcgacgcctgatgcttctgattggcgcagcactaacactgtggacttagacgagactgcccgcatagttgaagagctgattgcagctggtgtcaacggtattttaagtatgggtacttttggtgagtgcgccacgttgacctgggaggagaaacgtgattatgtttcgacggttgtcgagaccattcgtggtcgtgtgccttatttctgtggcacgacggccctgaatacccgagaagtcatccgccagacccgagagcttatcgatattggcgctaacggcaccatgctaggcgtgccgatgtgggtgaagatggacctgcccacagcggtccagttctatcgtgatgttgcaggcgcggtaccggaggctgccattgcgatttacgccaaccccgaagcattcaaattcgacttccctcgcccattttgggcagagatgtccaaaattcctcaggtagtgactgccaagtatctaggcatcggaatgcttgacttggacctgaaattggcgcctaacatccgcttccttccacacgaggacgactattacgcggccgcacgcatcaatcccgagcgcataaccgcgttctggtcaagcggggccatgtgcggcccggctaccgctatcatgttgcgtgatgaagtggagcgggccaagagtaccggtgactggatcaaggccaaagccatctccgatgatatgcgtgcagccgattcgacattgtttccgcgtggcgacttttcggagttctcgaagtataacatcgggcttgaaaaggcacggatggacgcggctggttggctcaaggctggtccctgccgtcctccctacaatcttgttccagaagattacctcgttggtgcacagaaatcaggcaaggcgtgggccgcgctgcacgctaaatacagtaaataattaaagtagttcacctccgcaggcctgagcgcgagggttacgaacacgctgagcggtgcgggaagtaagtgagttaaagctcatctcttgcgccaggcactgctagatcagcaaagttagctgatctggcagtctcgaaaattttggcgaaagctgatcttaggaatgcgggataaaggcagtacaccgtaacgatgggggtgtgccgttcatgttgaacgacgccgctattgcgccgacttctcttcttcggagtgtttgattgtgattgtcgatttttatttcgattttttgagtccgttctcttacttggccaatcagcgcttgtcaaagcttgcgcaagattatggccttaccattcgttataacgcgatcgatttggcgcgggtcaaaatagccattggaaacgttggtccatccaatcgcgacttgaaagtcaaattggactatttgaaagtagatttgcaacggtgggcccagctttacggaataccgctggtattcccagctaactacaacagccgacggatgaatattgggttttattactcgggggccgaggcgcaggccgctgcctatgtgaatgtagtatttaatgcggtttggggagaaggcatagctccagatttggaaagcttgcctgccctggtatctgaaaagctaggctgggatcgtagtgctttcgagcactttctcagcagcaatgccgcaacagagaggtatgacgagcagacacatgccgccatcgagcgcaaggtgttcggtgtgccaacgatgtttttgggcgatgaaatgtggtgggggaacgaccgtctattcatgctcgagagcgcaatggggcgcttgtgccggcaaaatgccgatttaagtagttgatga BBa_J23114_sequence 1 tttatggctagctcagtcctaggtacaatgctagc BBa_K957006_sequence 1 tttatggctagctcagtcctaggtacaatgctagctactagagtaagccggatttatggaacttctcatacagccaaacaatcgcctcattagctttagtcccggcgccaaccttctggaagtgcttcgcgaaaacggtgtcgctatttcctacagttgtatgtctgggcgttgcggaacctgccgctgtcgggttacagatggcagtgtaattgattcgggggcgggaagcgggttaccaaacctcgtggacgagcattatgtgctcgcctgtcagtcagtacttactcacaattgcgcgatcgaaatcccagaaaccgacgaaatcgtcacccacccggcgagaatcatcaagggcactgtggtcgccgtcgagtcgcccactcacgatatccgtcgcctacgcgtacgcctcgctaagcccttcgagttctcacccggacagtacgcgacattgcagttcagtcctgagcatgcgcgtccgtattcaatggcaggtctgccagatgaccaagaaatggagttccacatacgcaaggtgccgggtgggcgcgtaacggagtatgttttcgagcacgtccgcgaaggtacaagcatcaagttgagcgggccacttggtacggcttatttgcgtcagaaccacaccgggccgatgctctgtgtgggcggtgggaccggactagcaccggtgctgtcgattgttcgcggcgcgctgaagttgggtatgacaaaccccatcctcctttatttcggagtgcgcagtcagcaagacctctacgacgcagagcgattgcacaaactcgccgctgatcaccctcaactgaccgtacacacggtaatcgcaatgggcccgattaatgagagtcagcgagccggtctagttaccgatgtgatcgaaaaagacatcatttcgctggctgggtggagggcctacctgtgcggcgcaccagcgatggttgaagcgctttgcaccgttaccaagcatcttggaatatcacccgaacatatttatgccgatgccttctatcccggtggaatctgaatagtccctttccctgcacctctgtccatcgaggactcatcaggaggatactcaaataagcgcaaataataacagtcgcgtcagtatttgcggcagcgaaatggtttccctctccctcatttaccccatctgaggatagttttatgacagaaaaatggattgaagcagtcgccctttctgacatcccagaaggtgatgtcctcggcgtgactgtcgagggcaaggagctggcgttgtacgaagtggaaggcgaaatctacgctaccgacaacctgtgcacgcatggtgccgcccgcatgagcgatggttatctcgaggggcgagaaatcgaatgccccttgcatcaaggtcggtttgacgtttgtacaggcagagccctctgcgcccccgtgacagagaacatcaaaacatatgcagtcaagattgagaacctgcgcgtaatgattgatttgagcggagagttttaataggaggtaccccggaccctagcgcgtaactaccccaattccatcttttttaggtgaaaacatgaattacaaaaacaaaatattggtgagtgaatctgggctgacccaaaagcacctgattcatggcgatgaagaacttttccagcacgaactgagaaccatttttgcgcggaactggctttttctcactcatgacagcctgattccatcccccggcgactatgttaccgcaaaaatgggtattgacgaggtcatcgtctctcggcagagcgacggttcgattcgtgccttcctgaacgtttgtcggcaccgtggcaagacgctggttaacgcggaagccggcaatgccaaaggtttcgtttgcagctatcacggctggggcttcggctccaacggtgaactgcagagcgttccattcgaaaaagagctgtacggcgagtcgctcaacaaaaaatgtctggggttgaaagaagtcgctcgcgtggagagcttccatggcttcatctatggttgcttcgatcaggaggcccctcctcttatggactatctgggtgacgctgcttggtacctagagcccatcttcaaacattcaggcggtttagaactggtcggtcctccaggcaaggttgtgatcaaggccaactggaaggcacccgcggaaaactttgtgggggatgcataccacgtgggttggacgcacgcgtcttcgcttcgctcgggagagtctatcttcgcgtcgctcgctggcaacgcagtgctgccccctgaaggtgcaggcttgcaaatgacctccaaatacggcagcggcatgggtgtgttgtgggacggatattcaggcgtgcatagcgcagacttggttccggagttgatggcattcggcggctctaagcaggaaaggctgaacaaagaaattggcgatgttcgcgcccggatttatcgcagccacctcaactgcaccgttttcccgaacaacagcatgctgacctgctcgggtgttttcaaagtatggaacccgatcgacgcaaacaccaccgaggtctggacctacgccattgtcgaaaaagacatgcccgaggatctcaagcgccgcttggccgactcggttcagcgtacgtttgggcctgctggcttctgggaaagcgacgacaatgacaatatggaaacagcgtcgcaaaacggcaagaaatatcaatccagagatagtgatctgctttcaaaccttggtttcggtaaggacgtatacggcgacgcggtctatcctggcgtcgtcggcaaatcggcgatcggcgagaccagttatcgtggtttctaccgggcttaccaggcacacgtcagcagctccaactgggctgagttcgaggatgcctctagtacttggcataccgaactgacgaagactactgatcgctaacagacgagtcgaccatgatgatcaatattcaagaagacaagctggtctccgcccacgacgcccaagagtttcttcgtttcttcaattgccacgacgcggctttgcaacaagaagccaccacgctgctgaaccgggaagcgcatctgttggacattcaggcttaccgggcttggttagagcactgcgtggggtcagaggttcaatatcaggtcatttcacgcgaactgcgcgccgcttccgagcgccgttataagctcaatgaagccatgaacgtttacaacgaaaattttcagcaactgaaagttcgaatcgagcatcaactggatccgcaaaactggagcaacagcccgaagctgcgctttactcgcttcatcaccaatgtccaggccgcaagggacgtagatgacgaagagctacttcacatccgctccaacgtcattctgcaccgggcacgacgtggcaatcaggtcgatgtcttctacgccgcccgggaagacaaatggaaacgtggcgaaggtggagtgcgaaaattggtccagcgattcgtggattacccagagcgcatacttcagacgcacaatctgatggtctttctgtgatccagtgaccacttttacaaatggtgactgctaccgcggtcaccattaatcaaaagggaatgtaagtgtatgggcaatcaacaagtcgtttcgataaccggtgccggctcaggaatcggtctcgaactggttcgatcctttaagttggccggttattgcgtatccgctctcgtgcgaaacgaggagcaagaggcgcttctttgcaatgagttcaaggacgcactcgagatcgtagtgggcgatgtccgggaccacgcgacaaatgagaagctgataaagcagacaattgatagattcggtcatctcgattgtttcatcgcaaatgccggtatttgggattacatgctgaatatcgaagagccttgggagaaaatctcgagcagttttgacgaaatattcgacatcaatgtcaagagctatttcagtggcatcagtgccgccctgcccgagctgaaaaagactaacggatcagtggtgatgaccgcttcggtgtcgtcccatgcggtcggtggtggtggttcttgctacatcgccagcaagcatgcggtgctgggtatggtcaaggctttggcctacgaattggcccccgaaattcgcgtgaacgctgtttcgcctggcggcaccgtgacgtctctgtgcggtcccgctagcgccggtttcgacaaaatgcacatgaaagacatgcccggcatcgacgatatgatcaaaggtctcacacctcttgggtttgcagccaagcccgaagacgtggtggcaccctatctgttgctggcttcgcgaaagcaaggaaaattcatcactggcaccgtgattagcattgatggcggtatggcgctcggtcgcaagtgagcttgtagccgatcagaagttagacacattttcaggtgacgccccatgaagacaaaactgtttatcaacaacgcctggatcgattccagtgaccagcagaccttcgagcgcaagcaccccgtcagcagcgaggtgatgactgagagcgcaaacgccacggtgacggacgcgataaaggcggcgcaagcggccgaggaggcattcaagacctggaaggacgtcggaccttcggagcgtcgccgccttctcctgaaggtcgccgatgtcatggaaagtaaaacaccgaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgggaggctgcctcgctggccacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgaccctacgtcagccggtcggtccgatcctaagcatcgttccatggaacggcaccgcagtgctggcggcacgagccatcgcttatccgctggtctgtggcaacacggtagtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctggcgtgctcaactacctcaactcttcgcctgaccgttcgcccgagatcgccgacgcactgatctccgccaaggagatccgccgcatcaacttcacgggttccacccgcgtgggcagcattatcgcgcagaaggccgcgcaacacctcaagcgctgcctactggagctcggcggcaagtccccgcttattgttttagatgacgcagacatcaacgcggcggtaaaggcagcggtgttcggtagctttctattccaaggtcagatctgcatgtccactgagcgcttggtggttgatgagaagatcgccgacgaatttgtcgctaggtttgtcgaaaaaactgagcgcttgagcgtgggcgacccgtgcctaactggcgactgcatcatcggcccgatggtctcgccaaattcgggcgagcggatcaatggtttgttcaaggacgcgatagataaaggggccaaagttgtttgcggcggcatggcccagggtgcggtcatgccggccacgatcctggatcacgtcaaatctgacatgcggatctacgatgaggagacctttggtcccatcaccgtggtgatccgttgcaaaggcgaagcagaggccatccgcattgccaacgacagcgtttatggtctgtcgtcgggcgtgtttggccgcgacatcaaccgtgctctgcgagtgggtatgtccatcgaatatggttgcgtacacatcaacggctcgaccgtccagaacgaggcgcaggctccttacggaggtaccaagaacaccggctacgggcgcttcgacggccgtgccgtgatcgacgagttcacagagctcaagtggctgaccattgaaccattcgagcagcaatatcccttctgaaagcactaactccaaggaatcaaactatgagtaagcaagctgcagttatcgagctcggatacatgggcatctcggtcaaggatcctgatgcgtggaaatcgtttgccatgaatatgctgggtctgcaagttctggatgagggtgagaaggaccgtttctatctgcggatggattactggcaccatcggatcgtagttcatcacagcgcagaggacgacttggaatacctaggctggcgtgtagccggcaagccggagttcgaagctctggggcaaaagctcattgatgccggttacaagatccgtgtctgcgacaaagttgaggctcaggagcgtatggtgttgggcctgatgaagacagaagatccaggcggcaacccgaccgagatattctgggggccccggatcgacatgagcaatccgttccatcccggccgccctctgcacggcaagtttgtgaccggtgaccagggcttgggccattgcatcgttcgccaaaccgacgtcgctgcggcccataagttctacagcctgctgggcttccgtggggacgtcgaataccgcattccattgcccaatggcatgactgccgaactgtcgttcatgcattgcaacgcccgtgatcactccattgcgtttggtgccatgcccgctgccaagcgcctcaatcacttgatgcttgagtacacccatatggaagacttgggatatacgcatcaacagttcgtgaagaacgaaattgacattgccttgcagcttggcattcacgccaacgacaaggcgctgacgttctacggcgcaacgccttcgggctggcttattgagcccggctggcgaggtgctacggccatagacgaagcggagtattacgtcggcgacatcttcggccatggcgtcgaggcacctggatatggcctggatgtaaaactgagttaaaagcgcttatgcgcacattgggtttctgacgttttctgcttcctatacagcacaataacaataacaataacaataacaactaagtggaatataataatgataaaaaaactgttccttacgtgctttgtttactgttcactcagcaccgcatgggccgaagaatcaccttggacataccgcatcggcatgagtaatattgcttttgatacaagcgccaaagtgtacttggatggccaccgtgtgccgggaggaagcgctgacgcgagcaataacaacgcgctcacattcgacttcggctacgccatcaacgaccagtggaatgcacgtttgattgtcggtattccgcctacaactaaagttaagggcgcaggcacacttccgggcattcagctggggaaaataacttatgctccaacaatattaacgttaaactataacctcccagcttttgggcccgttcgtcctcacatcggtgcgggagtcaattacacgcggattttggaaagcaaggacgctaatctaaaatcattcgatgccgaccacgcttggtcccccgcgctgcatgttggtgccgatattgacgtgagtcgcaactggttcgtcagcattgatatccggaagttatacctgaaaaccgacgcatcaggttacttagggccacaggaggctaaagcaaaggtaactcttgacccattgataacatcgatcgcgatcggacgccaattctgatgaccccttttaaggctctctatctatctaactgcaaagggtatttttatgttgaataaagttattaaaaccacgcgtcttaccgctgaagatatcaacggtgcctggactataatgcccacaccgtcgacgcctgatgcttctgattggcgcagcactaacactgtggacttagacgagactgcccgcatagttgaagagctgattgcagctggtgtcaacggtattttaagtatgggtacttttggtgagtgcgccacgttgacctgggaggagaaacgtgattatgtttcgacggttgtcgagaccattcgtggtcgtgtgccttatttctgtggcacgacggccctgaatacccgagaagtcatccgccagacccgagagcttatcgatattggcgctaacggcaccatgctaggcgtgccgatgtgggtgaagatggacctgcccacagcggtccagttctatcgtgatgttgcaggcgcggtaccggaggctgccattgcgatttacgccaaccccgaagcattcaaattcgacttccctcgcccattttgggcagagatgtccaaaattcctcaggtagtgactgccaagtatctaggcatcggaatgcttgacttggacctgaaattggcgcctaacatccgcttccttccacacgaggacgactattacgcggccgcacgcatcaatcccgagcgcataaccgcgttctggtcaagcggggccatgtgcggcccggctaccgctatcatgttgcgtgatgaagtggagcgggccaagagtaccggtgactggatcaaggccaaagccatctccgatgatatgcgtgcagccgattcgacattgtttccgcgtggcgacttttcggagttctcgaagtataacatcgggcttgaaaaggcacggatggacgcggctggttggctcaaggctggtccctgccgtcctccctacaatcttgttccagaagattacctcgttggtgcacagaaatcaggcaaggcgtgggccgcgctgcacgctaaatacagtaaataattaaagtagttcacctccgcaggcctgagcgcgagggttacgaacacgctgagcggtgcgggaagtaagtgagttaaagctcatctcttgcgccaggcactgctagatcagcaaagttagctgatctggcagtctcgaaaattttggcgaaagctgatcttaggaatgcgggataaaggcagtacaccgtaacgatgggggtgtgccgttcatgttgaacgacgccgctattgcgccgacttctcttcttcggagtgtttgattgtgattgtcgatttttatttcgattttttgagtccgttctcttacttggccaatcagcgcttgtcaaagcttgcgcaagattatggccttaccattcgttataacgcgatcgatttggcgcgggtcaaaatagccattggaaacgttggtccatccaatcgcgacttgaaagtcaaattggactatttgaaagtagatttgcaacggtgggcccagctttacggaataccgctggtattcccagctaactacaacagccgacggatgaatattgggttttattactcgggggccgaggcgcaggccgctgcctatgtgaatgtagtatttaatgcggtttggggagaaggcatagctccagatttggaaagcttgcctgccctggtatctgaaaagctaggctgggatcgtagtgctttcgagcactttctcagcagcaatgccgcaacagagaggtatgacgagcagacacatgccgccatcgagcgcaaggtgttcggtgtgccaacgatgtttttgggcgatgaaatgtggtgggggaacgaccgtctattcatgctcgagagcgcaatggggcgcttgtgccggcaaaatgccgatttaagtagttgatgatactagaggccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaa BBa_J01003_sequence 1 gccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaa BBa_K957005_sequence 1 tttatggctagctcagtcctaggtacaatgctagctactagagtaagccggatttatggaacttctcatacagccaaacaatcgcctcattagctttagtcccggcgccaaccttctggaagtgcttcgcgaaaacggtgtcgctatttcctacagttgtatgtctgggcgttgcggaacctgccgctgtcgggttacagatggcagtgtaattgattcgggggcgggaagcgggttaccaaacctcgtggacgagcattatgtgctcgcctgtcagtcagtacttactcacaattgcgcgatcgaaatcccagaaaccgacgaaatcgtcacccacccggcgagaatcatcaagggcactgtggtcgccgtcgagtcgcccactcacgatatccgtcgcctacgcgtacgcctcgctaagcccttcgagttctcacccggacagtacgcgacattgcagttcagtcctgagcatgcgcgtccgtattcaatggcaggtctgccagatgaccaagaaatggagttccacatacgcaaggtgccgggtgggcgcgtaacggagtatgttttcgagcacgtccgcgaaggtacaagcatcaagttgagcgggccacttggtacggcttatttgcgtcagaaccacaccgggccgatgctctgtgtgggcggtgggaccggactagcaccggtgctgtcgattgttcgcggcgcgctgaagttgggtatgacaaaccccatcctcctttatttcggagtgcgcagtcagcaagacctctacgacgcagagcgattgcacaaactcgccgctgatcaccctcaactgaccgtacacacggtaatcgcaatgggcccgattaatgagagtcagcgagccggtctagttaccgatgtgatcgaaaaagacatcatttcgctggctgggtggagggcctacctgtgcggcgcaccagcgatggttgaagcgctttgcaccgttaccaagcatcttggaatatcacccgaacatatttatgccgatgccttctatcccggtggaatctgaatagtccctttccctgcacctctgtccatcgaggactcatcaggaggatactcaaataagcgcaaataataacagtcgcgtcagtatttgcggcagcgaaatggtttccctctccctcatttaccccatctgaggatagttttatgacagaaaaatggattgaagcagtcgccctttctgacatcccagaaggtgatgtcctcggcgtgactgtcgagggcaaggagctggcgttgtacgaagtggaaggcgaaatctacgctaccgacaacctgtgcacgcatggtgccgcccgcatgagcgatggttatctcgaggggcgagaaatcgaatgccccttgcatcaaggtcggtttgacgtttgtacaggcagagccctctgcgcccccgtgacagagaacatcaaaacatatgcagtcaagattgagaacctgcgcgtaatgattgatttgagcggagagttttaataggaggtaccccggaccctagcgcgtaactaccccaattccatcttttttaggtgaaaacatgaattacaaaaacaaaatattggtgagtgaatctgggctgacccaaaagcacctgattcatggcgatgaagaacttttccagcacgaactgagaaccatttttgcgcggaactggctttttctcactcatgacagcctgattccatcccccggcgactatgttaccgcaaaaatgggtattgacgaggtcatcgtctctcggcagagcgacggttcgattcgtgccttcctgaacgtttgtcggcaccgtggcaagacgctggttaacgcggaagccggcaatgccaaaggtttcgtttgcagctatcacggctggggcttcggctccaacggtgaactgcagagcgttccattcgaaaaagagctgtacggcgagtcgctcaacaaaaaatgtctggggttgaaagaagtcgctcgcgtggagagcttccatggcttcatctatggttgcttcgatcaggaggcccctcctcttatggactatctgggtgacgctgcttggtacctagagcccatcttcaaacattcaggcggtttagaactggtcggtcctccaggcaaggttgtgatcaaggccaactggaaggcacccgcggaaaactttgtgggggatgcataccacgtgggttggacgcacgcgtcttcgcttcgctcgggagagtctatcttcgcgtcgctcgctggcaacgcagtgctgccccctgaaggtgcaggcttgcaaatgacctccaaatacggcagcggcatgggtgtgttgtgggacggatattcaggcgtgcatagcgcagacttggttccggagttgatggcattcggcggctctaagcaggaaaggctgaacaaagaaattggcgatgttcgcgcccggatttatcgcagccacctcaactgcaccgttttcccgaacaacagcatgctgacctgctcgggtgttttcaaagtatggaacccgatcgacgcaaacaccaccgaggtctggacctacgccattgtcgaaaaagacatgcccgaggatctcaagcgccgcttggccgactcggttcagcgtacgtttgggcctgctggcttctgggaaagcgacgacaatgacaatatggaaacagcgtcgcaaaacggcaagaaatatcaatccagagatagtgatctgctttcaaaccttggtttcggtaaggacgtatacggcgacgcggtctatcctggcgtcgtcggcaaatcggcgatcggcgagaccagttatcgtggtttctaccgggcttaccaggcacacgtcagcagctccaactgggctgagttcgaggatgcctctagtacttggcataccgaactgacgaagactactgatcgctaacagacgagtcgaccatgatgatcaatattcaagaagacaagctggtctccgcccacgacgcccaagagtttcttcgtttcttcaattgccacgacgcggctttgcaacaagaagccaccacgctgctgaaccgggaagcgcatctgttggacattcaggcttaccgggcttggttagagcactgcgtggggtcagaggttcaatatcaggtcatttcacgcgaactgcgcgccgcttccgagcgccgttataagctcaatgaagccatgaacgtttacaacgaaaattttcagcaactgaaagttcgaatcgagcatcaactggatccgcaaaactggagcaacagcccgaagctgcgctttactcgcttcatcaccaatgtccaggccgcaagggacgtagatgacgaagagctacttcacatccgctccaacgtcattctgcaccgggcacgacgtggcaatcaggtcgatgtcttctacgccgcccgggaagacaaatggaaacgtggcgaaggtggagtgcgaaaattggtccagcgattcgtggattacccagagcgcatacttcagacgcacaatctgatggtctttctgtgatccagtgaccacttttacaaatggtgactgctaccgcggtcaccattaatcaaaagggaatgtaagtgtatgggcaatcaacaagtcgtttcgataaccggtgccggctcaggaatcggtctcgaactggttcgatcctttaagttggccggttattgcgtatccgctctcgtgcgaaacgaggagcaagaggcgcttctttgcaatgagttcaaggacgcactcgagatcgtagtgggcgatgtccgggaccacgcgacaaatgagaagctgataaagcagacaattgatagattcggtcatctcgattgtttcatcgcaaatgccggtatttgggattacatgctgaatatcgaagagccttgggagaaaatctcgagcagttttgacgaaatattcgacatcaatgtcaagagctatttcagtggcatcagtgccgccctgcccgagctgaaaaagactaacggatcagtggtgatgaccgcttcggtgtcgtcccatgcggtcggtggtggtggttcttgctacatcgccagcaagcatgcggtgctgggtatggtcaaggctttggcctacgaattggcccccgaaattcgcgtgaacgctgtttcgcctggcggcaccgtgacgtctctgtgcggtcccgctagcgccggtttcgacaaaatgcacatgaaagacatgcccggcatcgacgatatgatcaaaggtctcacacctcttgggtttgcagccaagcccgaagacgtggtggcaccctatctgttgctggcttcgcgaaagcaaggaaaattcatcactggcaccgtgattagcattgatggcggtatggcgctcggtcgcaagtgagcttgtagccgatcagaagttagacacattttcaggtgacgccccatgaagacaaaactgtttatcaacaacgcctggatcgattccagtgaccagcagaccttcgagcgcaagcaccccgtcagcagcgaggtgatgactgagagcgcaaacgccacggtgacggacgcgataaaggcggcgcaagcggccgaggaggcattcaagacctggaaggacgtcggaccttcggagcgtcgccgccttctcctgaaggtcgccgatgtcatggaaagtaaaacaccgaagttcatcgaagtgatggccatggaggtgggagcttccgccctttgggccggattcaacgtccatgcgtctgccaatgtgttccgggaggctgcctcgctggccacccaaattcagggtgaaaccatcccaacggacaaagccgaaacgctctcaatgaccctacgtcagccggtcggtccgatcctaagcatcgttccatggaacggcaccgcagtgctggcggcacgagccatcgcttatccgctggtctgtggcaacacggtagtgttcaaaggctctgaatttagtcccgcgacgcatgccctgatcacccagtgcgtgcaggaagccgggctgcccgctgg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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z